General Information of the Cell Line
Cell Line ID
CL000118
Cell Line Name
U-251MG
Synonyms
U-251 MG
U-251-MG
U-251_MG
U251-MG
U251MG
U-251
U251
U251n
U251N
251 MG
251MG
251 MG(6)
Category
Cancer cell line
Organism
Homo sapiens (Human)
Cellosaurus ID
CVCL_0021
Tissue Information
Brain
Disease
Astrocytoma (NCIt: C60781)
Astrocytoma (ORDO: Orphanet_94)
Map of Molecular Bioactivity Related to the Cell Line
Map of Molecular Bioactivity Related to the Cell Line

Cell Line
Protein
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Cell Line
Protein ID: PT02791, Hypoxia-inducible factor 1-alpha
 Protein Info 
Compound ID Compound Name Compound Formula
CP0093295
 Compound Info 
(S)-10-Dimethylaminomethyl-4-ethyl-4,9-dihydroxy-1,12-dihydro-4H-2-oxa-6,12a-diaza-dibenzo[b,h]fluorene-3,13-dione; hydrochloride
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C23H23N3O5
  3
1
EC50 = 12 nM
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2
IC50 = 60 nM
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3
IC50 > 3000 nM
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CP0849235
 Compound Info 
Rac-3-((3aSR,6SR,6aSR,12aRS,13aSR,13bRS)-6-(4-bromophenyl)-12a-methyl-1,3,7,12-tetraoxo-3a,4,6a,7,12,12a,13,13a-octahydro-1H-anthra[2,3-e]isoindol-2(3H,6H,13bH)-yl)propanoic acid
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C30H26BrNO6
  1
1
EC50 = 1400 nM
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CP0849525
 Compound Info 
Rac-(3aSR,6SR,6aSR,12aRS,13aSR,13bRS)-12a-methyl-6-(4'-(trifluoromethyl)phenyl-3a,4,6,6a,13,13a-hexahydro-1H-anthra[2,3-e]isoindole-1,3,7,12(2H,12aH,13bH)-tetraone
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C28H22F3NO4
  1
1
EC50 = 2300 nM
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CP0926433
 Compound Info 
Rac-3-((3aS,6S,6aS,12aR,13aS,13bR)-6-(4-fluorophenyl)-12a-methyl-1,3,7,12-tetraoxo-3a,4,6a,7,12,12a,13,13a-octahydro-1H-anthra[2,3-e]isoindol-2(3H,6H,13bH)-yl)propanoic acid
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C30H26FNO6
  1
1
EC50 = 4100 nM
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CP0893296
 Compound Info 
Rac-(3aSR,6SR,6aSR,12aRS,13aSR,13bRS)-8-methoxy-12a-methyl-6-(4-(trifluoromethyl)phenyl)-3a,4,6,6a,13,13a-hexahydro-1H-anthra[2,3-e]isoindole-1,3,7,12(2H,12aH,13bH)-tetraone
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C29H24F3NO5
  1
1
EC50 = 6700 nM
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CP0962209
 Compound Info 
Rac-(3aSR,6SR,6aSR,13aSR,13bRS)-11-hydroxy-6a-methyl-6-(4-(trifluoromethyl)phenyl)-3a,4,6,6a,13,13a-hexahydro-1H-anthra[2,3-e]isoindole-1,3,7,12(2H,12aH,13bH)-tetraone
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C28H22F3NO5
  1
1
EC50 = 6700 nM
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CP0967690
 Compound Info 
Rac-(3aSR,6SR,6aSR,12aRS,13aSR,13bRS)-6a,12a-dimethyl-6-(4-(trifluoromethyl)phenyl)-3a,4,6,6a,13,13a-hexahydro-1H-anthra[2,3-e]isoindole-1,3,7,12(2H,12aH,13bH)-tetraone
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C29H24F3NO4
  1
1
EC50 = 7800 nM
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CP0957928
 Compound Info 
Rac-3-((3aSR,6SR,6aSR,12aRS,13aSR,13bRS)-6-(4-bromophenyl)-8-methoxy-12a-methyl-1,3,7,12-tetraoxo-3a,4,6a,7,12,12a,13,13a-octahydro-1H-anthra[2,3-e]isoindol-2(3H,6H,13bH)-yl)propanoic acid
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C31H28BrNO7
  1
1
EC50 = 15500 nM
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CP0473514
 Compound Info 
2-chloro-N-(6-(4-hydroxy-6-(trifluoromethyl)pyrimidin-2-yl)pyridin-3-yl)acetamide
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C12H8ClF3N4O2
  2
1
IC50 = 800 nM
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2
IC50 = 8000 nM
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CP0386110
 Compound Info 
2-chloro-N-(6-(4-chloro-6-(trifluoromethyl)pyrimidin-2-yl)pyridin-3-yl)acetamide
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C12H7Cl2F3N4O
  2
1
IC50 = 1400 nM
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2
IC50 = 10000 nM
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CP0382925
 Compound Info 
2-chloro-N-(6-(4-(chloromethyl)-6-hydroxypyrimidin-2-yl)pyridin-3-yl)acetamide
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C12H10Cl2N4O2
  2
1
IC50 = 1500 nM
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2
IC50 = 5500 nM
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CP0428283
 Compound Info 
2-chloro-N-[6-(5-chloro-4-methyl-6-prop-2-enoxypyrimidin-2-yl)pyridin-3-yl]acetamide
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C15H14Cl2N4O2
  2
1
IC50 = 1500 nM
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2
IC50 = 8000 nM
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CP0481249
 Compound Info 
2-chloro-N-(6-(5-ethyl-4-hydroxy-6-methylpyrimidin-2-yl)pyridin-3-yl)acetamide
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C14H15ClN4O2
  2
1
IC50 = 1500 nM
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2
IC50 = 5500 nM
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CP0499868
 Compound Info 
2-chloro-N-[6-(5-ethyl-4-methyl-6-prop-2-enoxypyrimidin-2-yl)pyridin-3-yl]acetamide
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C17H19ClN4O2
  2
1
IC50 = 1800 nM
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2
IC50 = 22000 nM
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CP0382926
 Compound Info 
2-chloro-N-(6-(5-fluoro-4-hydroxy-6-methylpyrimidin-2-yl)pyridin-3-yl)acetamide
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C12H10ClFN4O2
  2
1
IC50 = 2000 nM
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2
IC50 = 6000 nM
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CP0499864
 Compound Info 
2-chloro-N-[6-[4-prop-2-enoxy-6-(trifluoromethyl)pyrimidin-2-yl]pyridin-3-yl]acetamide
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C15H12ClF3N4O2
  2
1
IC50 = 2000 nM
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2
IC50 = 30000 nM
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CP0388210
 Compound Info 
2-chloro-N-(6-(4-methoxy-6-(trifluoromethyl)pyrimidin-2-yl)pyridin-3-yl)acetamide
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C13H10ClF3N4O2
  2
1
IC50 = 3500 nM
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2
IC50 = 15000 nM
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CP0382924
 Compound Info 
N-[6-(5-benzyl-4-methyl-6-oxo-1H-pyrimidin-2-yl)pyridin-3-yl]-2-chloroacetamide
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C19H17ClN4O2
  2
1
IC50 = 4000 nM
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2
IC50 = 10000 nM
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CP0400148
 Compound Info 
2-chloro-N-(6-(5-chloro-4-hydroxy-6-methylpyrimidin-2-yl)pyridin-3-yl)acetamide
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C12H10Cl2N4O2
  1
1
IC50 = 5500 nM
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CP0386127
 Compound Info 
2-(5-aminopyridin-2-yl)-6-(chloromethyl)pyrimidin-4-ol
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C10H9ClN4O
  2
1
IC50 = 6000 nM
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2
IC50 = 22000 nM
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CP0379327
 Compound Info 
2-chloro-N-(6-(4-ethoxy-6-(trifluoromethyl)pyrimidin-2-yl)pyridin-3-yl)acetamide
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C14H12ClF3N4O2
  1
1
IC50 = 8000 nM
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CP0373648
 Compound Info 
2-(5-aminopyridin-2-yl)-5-ethyl-6-methylpyrimidin-4-ol
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C12H14N4O
  1
1
IC50 > 30000 nM
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CP0373649
 Compound Info 
2-(5-aminopyridin-2-yl)-5-benzyl-6-methylpyrimidin-4-ol
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C17H16N4O
  1
1
IC50 > 30000 nM
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CP0377229
 Compound Info 
2-(5-aminopyridin-2-yl)-6-(trifluoromethyl)pyrimidin-4-ol
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C10H7F3N4O
  1
1
IC50 > 30000 nM
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CP0377230
 Compound Info 
2-(5-aminopyridin-2-yl)-5-chloro-6-methylpyrimidin-4-ol
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C10H9ClN4O
  1
1
IC50 > 30000 nM
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CP0377252
 Compound Info 
2-chloro-2,2-difluoro-N-(6-(4-hydroxy-6-(trifluoromethyl)pyrimidin-2-yl)pyridin-3-yl)acetamide
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C12H6ClF5N4O2
  1
1
IC50 > 30000 nM
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CP0379326
 Compound Info 
6-(4-(allyloxy)-5-chloro-6-methylpyrimidin-2-yl)pyridin-3-amine
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C13H13ClN4O
  1
1
IC50 > 30000 nM
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CP0382927
 Compound Info 
2-chloro-N-(6-(4-hydroxy-5,6-dimethylpyrimidin-2-yl)pyridin-3-yl)acetamide
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C13H13ClN4O2
  1
1
IC50 > 30000 nM
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CP0386078
 Compound Info 
2-(5-aminopyridin-2-yl)-5,6-dimethylpyrimidin-4-ol
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C11H12N4O
  1
1
IC50 > 30000 nM
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CP0386081
 Compound Info 
6-(4-ethoxy-6-(trifluoromethyl)pyrimidin-2-yl)pyridin-3-amine
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C12H11F3N4O
  1
1
IC50 > 30000 nM
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CP0400150
 Compound Info 
6-(4-(allyloxy)-6-(trifluoromethyl)pyrimidin-2-yl)pyridin-3-amine
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C13H11F3N4O
  1
1
IC50 > 30000 nM
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CP0400151
 Compound Info 
6-(4-methoxy-6-(trifluoromethyl)pyrimidin-2-yl)pyridin-3-amine
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C11H9F3N4O
  1
1
IC50 > 30000 nM
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CP0425829
 Compound Info 
6-(4-(allyloxy)-5-ethyl-6-methylpyrimidin-2-yl)pyridin-3-amine
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C15H18N4O
  1
1
IC50 > 30000 nM
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CP0473513
 Compound Info 
2-(5-aminopyridin-2-yl)-5-fluoro-6-methylpyrimidin-4-ol
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C10H9FN4O
  1
1
IC50 > 30000 nM
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Protein ID: PT00844, Platelet-derived growth factor receptor beta
 Protein Info 
Protein ID: PT00839, Poly [ADP-ribose] polymerase 1
 Protein Info 
Protein ID: PT04559, Splicing factor 3B subunit 3
 Protein Info 
Protein ID: PT00864, Vascular endothelial growth factor receptor 2
 Protein Info 
Cell Viability or Cytotoxicity Assay
Compound ID Compound Name Compound Formula
CP0006369
 Compound Info 
6-[3-[4-[1-(5-cyanopentyl)indol-3-yl]-1-hydroxy-2,5-dioxopyrrol-3-yl]indol-1-yl]hexanenitrile
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 C32H31N5O3  2
1
GI50 = 4650 nM
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2
LC50 > 100000 nM
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CP0018072
 Compound Info 
US10662186, Compound 63
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 C27H32F2N8  1
1
IC50 = 135 nM
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CP0024501
 Compound Info 
(1S,5S,6R,9S,15E,20R)-6-[(2S)-butan-2-yl]-5-hydroxy-20-(2-methylsulfanylethyl)-2-oxa-11,12-dithia-7,19,22-triazabicyclo[7.7.6]docos-15-ene-3,8,18,21-tetrone
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 C23H37N3O6S3  1
1
GI50 = 4.4 nM
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CP0073095
 Compound Info 
2,4-difluoro-N-[2-methoxy-5-[4-methyl-2-(methylamino)-8-(oxan-4-yloxy)quinazolin-6-yl]pyridin-3-yl]benzenesulfonamide
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 C27H27F2N5O5S  1
1
IC50 = 700 nM
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CP0077619
 Compound Info 
Andrographolide
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 C20H30O5  1
1
GI50 = 3000 nM
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CP0082019
 Compound Info 
(2R)-2-amino-3-[(triphenylmethyl)sulfanyl]propanoic acid
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 C22H21NO2S  1
1
GI50 = 1000 nM
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CP0086475
 Compound Info 
2-[(4-methoxyphenyl)-diphenylmethyl]sulfanylethanamine
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 C22H23NOS  1
1
GI50 = 50 nM
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CP0091315
 Compound Info 
US9422297, AAG145-136
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 C17H13ClN4  1
1
GI50 = 184 nM
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CP0093295
 Compound Info 
(S)-10-Dimethylaminomethyl-4-ethyl-4,9-dihydroxy-1,12-dihydro-4H-2-oxa-6,12a-diaza-dibenzo[b,h]fluorene-3,13-dione; hydrochloride
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 C23H23N3O5  1
1
IC50 = 500 nM
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CP0140706
 Compound Info 
2-[[5-chloro-2-[4-[[[2-[2-[[(E)-2-cyano-4-methylpent-2-enoyl]amino]ethylamino]-3,4-dioxocyclobuten-1-yl]amino]methyl]anilino]pyrimidin-4-yl]amino]-N-methylbenzamide
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 C32H32ClN9O4  1
1
IC50 = 9800 nM
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CP0176598
 Compound Info 
N-[4-[2-[(2S,3S,5R)-2-[[tert-butyl(dimethyl)silyl]oxymethyl]-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-3-yl]-1,3-dithian-2-yl]phenyl]-N'-hydroxyoctanediamide
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 C34H52N4O7S2Si  1
1
GI50 = 3981.07 nM
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CP0345034
 Compound Info 
[(2R)-1-(4-tert-butylphenoxy)-3-(2-nitroimidazol-1-yl)propan-2-yl] 4-[(2-hydroxy-5-oxocyclopenta-1,3-dien-1-yl)methylideneamino]benzoate
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 C29H28N4O7  1
1
GI50 = 17000 nM
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CP0370065
 Compound Info 
5-(4-chlorophenyl)-2-(4-methoxyphenyl)-[1,3]thiazolo[3,2-b][1,2,4]triazole
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 C17H12ClN3OS  2
1
GI50 = 1030 nM
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2
LC50 = 5530 nM
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CP0421570
 Compound Info 
N-(5-methoxynaphthalen-2-yl)-N,2-dimethyl-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-amine
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 C20H21N3O  1
1
GI50 < 10 nM
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CP0424792
 Compound Info 
2-[[5-chloro-2-[4-[[[2-[2-[[(E)-2-cyano-3-cyclopropylprop-2-enoyl]amino]ethylamino]-3,4-dioxocyclobuten-1-yl]amino]methyl]anilino]pyrimidin-4-yl]amino]-N-methylbenzamide
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 C32H30ClN9O4  1
1
IC50 = 1200 nM
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CP0425261
 Compound Info 
N-(1-benzofuran-5-yl)-N,2,6-trimethylfuro[2,3-d]pyrimidin-4-amine
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 C17H15N3O2  1
1
GI50 = 84 nM
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CP0439788
 Compound Info 
3-Bromo-9,9-dimethyl-5,6,8,9,10,11-hexahydro-12Hazepino[ 3',4':4,5]pyrrolo[2,1-a]isoquinolin-12-one
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 C18H19BrN2O  1
1
IC50 = 18140 nM
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CP0452777
 Compound Info 
1-[3-[4-[1-adamantyl(phenyl)methyl]phenyl]propyl]-4-methylpiperazine
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 C31H42N2  1
1
GI50 = 3.62 nM
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CP0487026
 Compound Info 
(1S,5R,6S,9S,15E,20S)-5-hydroxy-20-(2-methylsulfanylethyl)-6-propan-2-yl-2-oxa-11,12-dithia-7,19,22-triazabicyclo[7.7.6]docos-15-ene-3,8,18,21-tetrone
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 C22H35N3O6S3  1
1
GI50 = 410 nM
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CP0503552
 Compound Info 
3-Fluoro-9,9-dimethyl-5,6,8,9,10,11-hexahydro-12Hazepino[3',4':4,5]pyrrolo[2,1-a]isoquinolin-12-one
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 C18H19FN2O  1
1
IC50 > 100000 nM
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CP0516899
 Compound Info 
4-(6-methoxy-3,4-dihydro-2H-quinolin-1-yl)-2-methylquinazoline
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 C19H19N3O  1
1
IC50 = 3.1 nM
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CP0517268
 Compound Info 
1-[4-[1-adamantyl(phenyl)methyl]phenyl]-4-ethylpiperazine
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 C29H38N2  2
1
GI50 = 4230 nM
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2
LC50 = 11950 nM
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CP0524982
 Compound Info 
1-[4-[1-adamantyl(phenyl)methyl]phenyl]-4-methylpiperazine
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 C28H36N2  2
1
GI50 = 4000 nM
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2
LC50 = 9140 nM
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CP0554563
 Compound Info 
(1S,10S,16E,21R)-21-propan-2-yl-2-oxa-12,13-dithia-5,8,20,23-tetrazabicyclo[8.7.6]tricos-16-ene-3,6,9,19,22-pentone
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 C19H28N4O6S2  1
1
GI50 = 17000 nM
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CP0560539
 Compound Info 
2-(benzoylcarbamothioylamino)-6-tert-butyl-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxamide
   Show/Hide
 C21H25N3O2S2  1
1
GI50 = 70180 nM
   TI
   LI
   LO
   TS
CP0565042
 Compound Info 
(1S,7S,10S,16E,21R)-7-methyl-21-propan-2-yl-2-oxa-12,13-dithia-5,8,20,23-tetrazabicyclo[8.7.6]tricos-16-ene-3,6,9,19,22-pentone
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 C20H30N4O6S2  1
1
GI50 = 17000 nM
   TI
   LI
   LO
   TS
CP0897293
 Compound Info 
1-(7-Benzyl-2-methyl-5H-pyrrolo[3,2-d]pyrimidin-4-yl)-6-methoxy-1,2,3,4-tetrahydroquinoline hydrochloride
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 C24H25ClN4O  1
1
GI50 = 316 nM
   TI
   LI
   LO
   TS
Protein family and CMB statistics Related to the Cell Line
Cell Lines Derived from the same Tissue Source (Brain)
Cell Line Name Organism Disease
TS603
 Cell Line Info 
Homo sapiens (Human) Anaplastic oligodendroglioma
H4
 Cell Line Info 
Homo sapiens (Human) Astrocytoma
1321N1
 Cell Line Info 
Homo sapiens (Human) Astrocytoma
SNB-19
 Cell Line Info 
Homo sapiens (Human) Astrocytoma
U-373MG ATCC
 Cell Line Info 
Homo sapiens (Human) Astrocytoma
LN-308
 Cell Line Info 
Homo sapiens (Human) Astrocytoma
CCF-STTG1
 Cell Line Info 
Homo sapiens (Human) Astrocytoma
LN-319
 Cell Line Info 
Homo sapiens (Human) Astrocytoma
P2X7-1321N1
 Cell Line Info 
Homo sapiens (Human) Astrocytoma
MDA-MB-361
 Cell Line Info 
Homo sapiens (Human) Breast adenocarcinoma
JEG-3
 Cell Line Info 
Homo sapiens (Human) Gestational choriocarcinoma
D3 [Human glioblastoma]
 Cell Line Info 
Homo sapiens (Human) Glioblastoma
LN-229
 Cell Line Info 
Homo sapiens (Human) Glioblastoma
LN-18
 Cell Line Info 
Homo sapiens (Human) Glioblastoma
A-172
 Cell Line Info 
Homo sapiens (Human) Glioblastoma
UC-11MG
 Cell Line Info 
Homo sapiens (Human) Glioblastoma
T98G
 Cell Line Info 
Homo sapiens (Human) Glioblastoma
LN229-V6R
 Cell Line Info 
Homo sapiens (Human) Glioblastoma
SF539
 Cell Line Info 
Homo sapiens (Human) Gliosarcoma
MED1
 Cell Line Info 
Homo sapiens (Human) Medulloblastoma, non-WNT/non-SHH, group 4
Kelly
 Cell Line Info 
Homo sapiens (Human) Neuroblastoma
PFSK-1
 Cell Line Info 
Homo sapiens (Human) Primitive neuroectodermal tumor
DU145
 Cell Line Info 
Homo sapiens (Human) Prostate carcinoma
DU145-TxR
 Cell Line Info 
Homo sapiens (Human) Prostate carcinoma
GL261
 Cell Line Info 
Mus musculus (Mouse) Mouse glioblastoma
C6
 Cell Line Info 
Rattus norvegicus (Rat) Rat malignant glioma
C6-BU-1
 Cell Line Info 
Rattus norvegicus (Rat) Rat malignant glioma
RG2
 Cell Line Info 
Rattus norvegicus (Rat) Rat malignant glioma
ReNcell VM
 Cell Line Info 
Homo sapiens (Human) -
hCMEC/D3
 Cell Line Info 
Homo sapiens (Human) -
BV-2
 Cell Line Info 
Mus musculus (Mouse) -
C17.2
 Cell Line Info 
Mus musculus (Mouse) -
ScGT1
 Cell Line Info 
Mus musculus (Mouse) -
bEnd.4
 Cell Line Info 
Mus musculus (Mouse) -
N39
 Cell Line Info 
Mus musculus (Mouse) -
GT1-7
 Cell Line Info 
Mus musculus (Mouse) -
N27-A
 Cell Line Info 
Rattus norvegicus (Rat) -
RBE4
 Cell Line Info 
Rattus norvegicus (Rat) -