General Information of the Cell Line
Cell Line ID
CL000218
Cell Line Name
BT-474
Synonyms
Bt-474
BT474
Category
Cancer cell line
Organism
Homo sapiens (Human)
Cellosaurus ID
CVCL_0179
Tissue Information
Breast
Disease
Invasive breast carcinoma of no special type (NCIt: C4194)
Map of Molecular Bioactivity Related to the Cell Line
Map of Molecular Bioactivity Related to the Cell Line

Cell Line
Protein
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Cell Line
Protein ID: PT01093, 3-phosphoinositide-dependent protein kinase 1
 Protein Info 
Compound ID Compound Name Compound Formula
CP0473312
 Compound Info 
7-methyl-2-((7-methyl-[1,2,4]triazolo[1,5-a]pyridin-6-yl)amino)-9-(tetrahydro-2H-pyran-4-yl)-7,9-dihydro-8H-purin-8-one
   Show/Hide
C18H20N8O2
  1
1
IC50 > 8300 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Protein ID: PT00847, CDw156
 Protein Info 
Protein ID: PT02825, Fatty acid synthase
 Protein Info 
Protein ID: PT00962, Glycogen synthase kinase-3 beta
 Protein Info 
Protein ID: PT01045, Phosphoinositide 3-kinase alpha
 Protein Info 
Protein ID: PT03525, Phosphoinositide-3-kinase catalytic alpha polypeptide
 Protein Info 
Protein ID: PT00845, RAC-alpha serine/threonine-protein kinase
 Protein Info 
Protein ID: PT01233, Receptor tyrosine-protein kinase erbB-2
 Protein Info 
Cell Viability or Cytotoxicity Assay
Compound ID Compound Name Compound Formula
CP0002912
 Compound Info 
4-[[2-[(4-aminocyclohexyl)amino]-9-hexylpurin-6-yl]amino]phenol
   Show/Hide
 C23H33N7O  1
1
GI50 = 7700 nM
   TI
   LI
   LO
   TS
CP0021627
 Compound Info 
4-(2-(4-amino-1,2,5-oxadiazol-3-yl)-1-ethyl-6-(pyrrolidin-1-ylmethyl)-1H-imidazo[4,5-c]pyridin-4-yl)-2-methylbut-3-yn-2-ol
   Show/Hide
 C20H25N7O2  1
1
IC50 = 1250 nM
   TI
   LI
   LO
   TS
CP0024313
 Compound Info 
[2-amino-4-(trifluoromethoxy)phenyl]-[4-[7-(4-methylpiperazin-1-yl)pyrido[3,2-d]pyrimidin-4-yl]piperidin-1-yl]methanone
   Show/Hide
 C25H28F3N7O2  1
1
IC50 > 30000 nM
   TI
   LI
   LO
   TS
CP0037802
 Compound Info 
4-(2-(4-amino-1,2,5-oxadiazol-3-yl)-6-((S)-2-amino-1-phenylethoxy)-1-ethyl-1H-imidazo[4,5-c]pyridin-4-yl)-2-methylbut-3-yn-2-ol
   Show/Hide
 C23H25N7O3  1
1
IC50 = 124 nM
   TI
   LI
   LO
   TS
CP0039472
 Compound Info 
4-N-[1-[(3-fluorophenyl)methyl]indazol-5-yl]-5-[(E)-piperidin-1-yliminomethyl]pyrimidine-4,6-diamine
   Show/Hide
 C24H25FN8  1
1
IC50 = 49 nM
   TI
   LI
   LO
   TS
CP0043499
 Compound Info 
3-((S)-2-amino-3-(1H-indol-3-yl)propoxy)-5-(3-methyl-1H-pyrazolo[4,3-b]pyrazin-5-yl)-6-(2-methylfuran-3-yl)pyridin-2-amine
   Show/Hide
 C27H26N8O2  1
1
IC50 = 310 nM
   TI
   LI
   LO
   TS
CP0046864
 Compound Info 
4-(2-(4-amino-1,2,5-oxadiazol-3-yl)-6-((R)-2-amino-3-phenylpropoxy)-1-ethyl-1H-imidazo[4,5-c]pyridin-4-yl)-2-methylbut-3-yn-2-ol
   Show/Hide
 C24H27N7O3  1
1
IC50 = 742 nM
   TI
   LI
   LO
   TS
CP0046866
 Compound Info 
4-(2-(4-amino-1,2,5-oxadiazol-3-yl)-1-ethyl-6-((methylamino)methyl)-1H-imidazo[4,5-c]pyridin-4-yl)-2-methylbut-3-yn-2-ol
   Show/Hide
 C17H21N7O2  1
1
IC50 = 439 nM
   TI
   LI
   LO
   TS
CP0046892
 Compound Info 
4-(2-(4-amino-1,2,5-oxadiazol-3-yl)-6-((R)-3-amino-1-phenylpropoxy)-1-ethyl-1H-imidazo[4,5-c]pyridin-4-yl)-2-methylbut-3-yn-2-ol
   Show/Hide
 C24H27N7O3  1
1
IC50 = 224 nM
   TI
   LI
   LO
   TS
CP0048166
 Compound Info 
2-[[5-cyano-4-[2,4-dichloro-5-[2-(diethylamino)ethoxy]phenyl]-7H-pyrrolo[2,3-d]pyrimidin-2-yl]sulfanyl]-N-methylacetamide
   Show/Hide
 C22H24Cl2N6O2S  1
1
GI50 = 78 nM
   TI
   LI
   LO
   TS
CP0057972
 Compound Info 
2-[[4-(2-chloro-4,5-dimethoxyphenyl)-5-cyano-7H-pyrrolo[2,3-d]pyrimidin-2-yl]sulfanyl]-N,N-dimethylacetamide
   Show/Hide
 C19H18ClN5O3S  1
1
GI50 = 34 nM
   TI
   LI
   LO
   TS
CP0069884
 Compound Info 
4-[2-(4-amino-1,2,5-oxadiazol-3-yl)-1-ethyl-7-(3-{[2-(4-methoxyphenyl)ethyl]amino}propoxy)-1H-imidazo[4,5-c]pyridin-4-yl]-2-methylbut-3-yn-2-ol
   Show/Hide
 C27H33N7O4  1
1
IC50 = 260 nM
   TI
   LI
   LO
   TS
CP0070350
 Compound Info 
(E)-4-(diethylamino)-1-[4-(3-ethynylanilino)-7,8-dihydropyrimido[5,4-g][1,4]benzoxazin-6-yl]but-2-en-1-one
   Show/Hide
 C26H27N5O2  1
1
IC50 = 550 nM
   TI
   LI
   LO
   TS
CP0088096
 Compound Info 
(2S)-2-(4-chlorophenyl)-1-[4-[(5R,7R)-7-hydroxy-5-methyl-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl]piperazin-1-yl]-3-(propan-2-ylamino)propan-1-one
   Show/Hide
 C24H32ClN5O2  1
1
IC50 = 1000 nM
   TI
   LI
   LO
   TS
CP0095882
 Compound Info 
(2S)-1-{[6-furan-3-yl-5-(3-methyl-2H-indazol-5-yl)pyridin-3-yl]oxy}-3-(1H-indol-3-yl)propan-2-amine
   Show/Hide
 C28H25N5O2  1
1
IC50 = 340 nM
   TI
   LI
   LO
   TS
CP0096184
 Compound Info 
(S)-1-(6-(furan-3-yl)-5-(3-methyl-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yloxy)-3-(1H-indol-3-yl)propan-2-amine
   Show/Hide
 C27H24N6O2  1
1
IC50 = 300 nM
   TI
   LI
   LO
   TS
CP0122572
 Compound Info 
4-(2-(4-amino-1,2,5-oxadiazol-3-yl)-6-((S)-3-amino-1-phenylpropoxy)-1-ethyl-1H-imidazo[4,5-c]pyridin-4-yl)-2-methylbut-3-yn-2-ol
   Show/Hide
 C24H27N7O3  1
1
IC50 = 1 nM
   TI
   LI
   LO
   TS
CP0128025
 Compound Info 
4-(2-(4-amino-1,2,5-oxadiazol-3-yl)-6-((dimethylamino)methyl)-1-ethyl-1H-imidazo[4,5-c]pyridin-4-yl)-2-methylbut-3-yn-2-ol
   Show/Hide
 C18H23N7O2  1
1
IC50 = 357 nM
   TI
   LI
   LO
   TS
CP0128460
 Compound Info 
3-((2S)-2-amino-3-(2-amino-5-(3-methyl-1H-pyrazolo[4,3-b]pyrazin-5-yl)-6-(2-methylfuran-3-yl)pyridin-3-yloxy)propyl)-1H-indole-2-carboxamide
   Show/Hide
 C28H27N9O3  1
1
IC50 > 10000 nM
   TI
   LI
   LO
   TS
CP0151263
 Compound Info 
(S)-1-(6-(furan-3-yl)-5-(3-methyl-1H-pyrazolo[4,3-d]pyrimidin-5-yl)pyridin-3-yloxy)-3-(1H-indol-3-yl)propan-2-amine
   Show/Hide
 C26H23N7O2  1
1
IC50 = 640 nM
   TI
   LI
   LO
   TS
CP0151265
 Compound Info 
(S)-1-(6-(furan-3-yl)-5-(3-methyl-1H-pyrazolo[4,3-b]pyridin-5-yl)pyridin-3-yloxy)-3-(1H-indol-3-yl)propan-2-amine
   Show/Hide
 C27H24N6O2  1
1
IC50 = 2100 nM
   TI
   LI
   LO
   TS
CP0163871
 Compound Info 
4-N-[1-[(3-fluorophenyl)methyl]indazol-5-yl]-5-[(E)-morpholin-4-yliminomethyl]pyrimidine-4,6-diamine
   Show/Hide
 C23H23FN8O  1
1
IC50 = 31 nM
   TI
   LI
   LO
   TS
CP0206212
 Compound Info 
4-[2-(4-amino-1,2,5-oxadiazol-3-yl)-7-(2-aminoethoxy)-1-ethyl-1H-imidazo[4,5-c]pyridin-4-yl]-2-methylbut-3-yn-2-ol
   Show/Hide
 C17H21N7O3  1
1
IC50 = 420 nM
   TI
   LI
   LO
   TS
CP0206214
 Compound Info 
4-[2-(4-amino-1,2,5-oxadiazol-3-yl)-7-(4-aminobutoxy)-1-ethyl-1H-imidazo[4,5-c]pyridin-4-yl]-2-methylbut-3-yn-2-ol
   Show/Hide
 C19H25N7O3  1
1
IC50 = 700 nM
   TI
   LI
   LO
   TS
CP0244689
 Compound Info 
4-(2-(4-amino-1,2,5-oxadiazol-3-yl)-6-(2-aminoethyl)-1-ethyl-1H-imidazo[4,5-c]pyridin-4-yl)-2-methylbut-3-yn-2-ol
   Show/Hide
 C17H21N7O2  1
1
IC50 = 1090 nM
   TI
   LI
   LO
   TS
CP0265167
 Compound Info 
3-((S)-2-amino-3-(1H-indol-3-yl)propoxy)-5-(3-methyl-1H-indazol-5-yl)-6-(2-methylfuran-3-yl)pyridin-2-amine
   Show/Hide
 C29H28N6O2  1
1
IC50 = 200 nM
   TI
   LI
   LO
   TS
CP0271240
 Compound Info 
4-(2-(4-amino-1,2,5-oxadiazol-3-yl)-1-ethyl-6-(2-(methylamino)ethoxy)-1H-imidazo[4,5-c]pyridin-4-yl)-2-methylbut-3-yn-2-ol
   Show/Hide
 C18H23N7O3  1
1
IC50 = 3190 nM
   TI
   LI
   LO
   TS
CP0274799
 Compound Info 
4-(2-(4-amino-1,2,5-oxadiazol-3-yl)-6-(3-aminopropyl)-1-ethyl-1H-imidazo[4,5-c]pyridin-4-yl)-2-methylbut-3-yn-2-ol
   Show/Hide
 C18H23N7O2  1
1
IC50 = 3520 nM
   TI
   LI
   LO
   TS
CP0281776
 Compound Info 
2-(4-((4-(1-(3-fluorobenzyl)-1H-indazol-5-ylamino)-6-aminopyrimidin-5-yl)methyleneamino)piperazin-1-yl)ethanol
   Show/Hide
 C25H28FN9O  1
1
IC50 = 14 nM
   TI
   LI
   LO
   TS
CP0293987
 Compound Info 
4-(2-(4-amino-1,2,5-oxadiazol-3-yl)-6-((S)-2-amino-3-phenylpropoxy)-1-ethyl-1H-imidazo[4,5-c]pyridin-4-yl)-2-methylbut-3-yn-2-ol
   Show/Hide
 C24H27N7O3  1
1
IC50 = 1500 nM
   TI
   LI
   LO
   TS
CP0353118
 Compound Info 
(S)-1-(6-(furan-3-yl)-2-methyl-5-(3-methyl-1H-pyrazolo[4,3-b]pyrazin-5-yl)pyridin-3-yloxy)-3-(1H-indol-3-yl)propan-2-amine
   Show/Hide
 C27H25N7O2  1
1
IC50 = 1260 nM
   TI
   LI
   LO
   TS
CP0432356
 Compound Info 
2-piperidin-4-ylethyl N-[4-[(1-benzylindazol-5-yl)amino]-5-ethyl-7aH-pyrrolo[2,3-d]pyrimidin-6-yl]carbamate
   Show/Hide
 C30H34N8O2  1
1
IC50 = 130 nM
   TI
   LI
   LO
   TS
CP0432357
 Compound Info 
(4-amino-4-methylcyclohexyl) N-[4-[(1-benzylindazol-5-yl)amino]-5-ethyl-7aH-pyrrolo[2,3-d]pyrimidin-6-yl]carbamate
   Show/Hide
 C30H34N8O2  1
1
IC50 = 130 nM
   TI
   LI
   LO
   TS
CP0433375
 Compound Info 
(E)-1-[4-(3-ethynylanilino)-8,9-dihydro-7H-pyrimido[5,4-h][1,5]benzoxazepin-6-yl]-4-piperidin-1-ylbut-2-en-1-one
   Show/Hide
 C28H29N5O2  1
1
IC50 > 10000 nM
   TI
   LI
   LO
   TS
CP0478189
 Compound Info 
4-(4-(cyclopentyloxy)-5-(2- methylbenzo[d]oxazol-6-yl)-7H- pyrrolo[2,3-d]pyrimidin-2- ylamino)-3-methoxy-N- methylbenzamide
   Show/Hide
 C28H28N6O4  1
1
IC50 = 6970 nM
   TI
   LI
   LO
   TS
CP0494681
 Compound Info 
2-imidazol-1-ylethyl N-[4-[(1-benzylindazol-5-yl)amino]-5-ethyl-7aH-pyrrolo[2,3-d]pyrimidin-6-yl]carbamate
   Show/Hide
 C28H27N9O2  1
1
IC50 = 860 nM
   TI
   LI
   LO
   TS
CP0496951
 Compound Info 
2-piperazin-1-ylethyl N-[4-[(1-benzylindazol-5-yl)amino]-5-ethyl-7aH-pyrrolo[2,3-d]pyrimidin-6-yl]carbamate
   Show/Hide
 C29H33N9O2  1
1
IC50 = 71 nM
   TI
   LI
   LO
   TS
CP0499124
 Compound Info 
piperidin-2-ylmethyl N-[4-[(1-benzylindazol-5-yl)amino]-5-ethyl-7aH-pyrrolo[2,3-d]pyrimidin-6-yl]carbamate
   Show/Hide
 C29H32N8O2  1
1
IC50 = 470 nM
   TI
   LI
   LO
   TS
CP0531857
 Compound Info 
(E)-1-[4-[3-chloro-4-[(3-fluorophenyl)methoxy]anilino]-8,9-dihydro-7H-pyrimido[5,4-h][1,5]benzoxazepin-6-yl]-4-morpholin-4-ylbut-2-en-1-one
   Show/Hide
 C32H31ClFN5O4  1
1
IC50 > 10000 nM
   TI
   LI
   LO
   TS
CP0532116
 Compound Info 
(E)-1-[4-[3-chloro-4-[(3-fluorophenyl)methoxy]anilino]-8,9-dihydro-7H-pyrimido[5,4-h][1,5]benzoxazepin-6-yl]-4-pyrrolidin-1-ylbut-2-en-1-one
   Show/Hide
 C32H31ClFN5O3  1
1
IC50 = 172 nM
   TI
   LI
   LO
   TS
CP0558106
 Compound Info 
(E)-1-[4-[3-chloro-4-[(3-fluorophenyl)methoxy]anilino]-8,9-dihydro-7H-pyrimido[5,4-h][1,5]benzoxazepin-6-yl]-4-(dimethylamino)but-2-en-1-one
   Show/Hide
 C30H29ClFN5O3  1
1
IC50 = 127 nM
   TI
   LI
   LO
   TS
CP0562111
 Compound Info 
(E)-1-[4-[3-chloro-4-[(3-fluorophenyl)methoxy]anilino]-7,8-dihydropyrimido[5,4-g][1,4]benzoxazin-6-yl]-4-(2-methylpiperidin-1-yl)but-2-en-1-one
   Show/Hide
 C33H33ClFN5O3  1
1
IC50 = 127 nM
   TI
   LI
   LO
   TS
CP0562232
 Compound Info 
(E)-1-[4-[3-chloro-4-[(3-fluorophenyl)methoxy]anilino]-7,8-dihydropyrimido[5,4-g][1,4]benzoxazin-6-yl]-4-(4-methylpiperidin-1-yl)but-2-en-1-one
   Show/Hide
 C33H33ClFN5O3  1
1
IC50 = 200 nM
   TI
   LI
   LO
   TS
CP0634648
 Compound Info 
N-((R)-Cyclopropyl(pyridin-2-yl)methyl)-3-(4-((endo)-3-hydroxy-8-azabicyclo[3.2.1]octan-8-yl)phenyl)-1H-indazole-5-carboxamide
   Show/Hide
 C30H31N5O2  1
1
GI50 = 15 nM
   TI
   LI
   LO
   TS
Protein family and CMB statistics Related to the Cell Line
Cell Lines Derived from the same Tissue Source (Breast)
Cell Line Name Organism Disease
MX-1
 Cell Line Info 
Homo sapiens (Human) Breast carcinoma
HCC1954
 Cell Line Info 
Homo sapiens (Human) Breast ductal carcinoma
HDQ-P1
 Cell Line Info 
Homo sapiens (Human) Breast ductal carcinoma
SUM159PT
 Cell Line Info 
Homo sapiens (Human) Breast pleomorphic carcinoma
HCC1806
 Cell Line Info 
Homo sapiens (Human) Breast squamous cell carcinoma, acantholytic variant
Hs 578T
 Cell Line Info 
Homo sapiens (Human) Invasive breast carcinoma of no special type
BT-20
 Cell Line Info 
Homo sapiens (Human) Invasive breast carcinoma of no special type
BT-549
 Cell Line Info 
Homo sapiens (Human) Invasive breast carcinoma of no special type
MCF-10A
 Cell Line Info 
Homo sapiens (Human) -
184B5
 Cell Line Info 
Homo sapiens (Human) -
UFH-001
 Cell Line Info 
Homo sapiens (Human) -
184A1N4
 Cell Line Info 
Homo sapiens (Human) -