Brief Information of the Cell Line and Protein
Cell Line ID: CL000006 , HEK293
Synonyms
Hek293
HEK-293
HEK/293
HEK 293
HEK;293
293
293 HEK
293 Ad5
Graham 293
Graham-293
Human Embryonic Kidney 293
Category Transformed cell line
Organism
Homo sapiens (Human)
Cellosaurus ID
CVCL_0045
Protein ID: PT04361 , Protein smoothened
Gene Name SMO
Organism Homo sapiens, Human
Protein Classification Membrane receptor
Uniprot ID Q99835
Table of Molecular Bioactivities Related to the Cell Line and Protein
Cell Line ID: CL000006  Protein ID: PT04361
 
Compound ID Compound Name Compound Formula
CP0915507
(S)-N-(2-(3-chlorophenyl)pyridin-3-yl)-1,3-dioxo-2-((1S,2R)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C27H24ClN5O3
 2
1
IC50 = 4 nM
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2
IC50 = 15 nM
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CP0656832
SID90341472
   Show/Hide
C23H27N5
 2
1
IC50 = 4.74 nM
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2
IC50 = 25 nM
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CP0472109
N-[[1-(2-methoxyphenyl)indazol-5-yl]methyl]-2-propylpentanamide
   Show/Hide
C23H29N3O2
 1
1
IC50 = 5 nM
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CP0479045
1-((1-(4-fluorophenyl)-5-methyl-1H-pyrazol-4-yl)methyl)-4-(3-methoxyphenyl)piperazine
   Show/Hide
C22H25FN4O
 2
1
IC50 = 5 nM
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2
IC50 = 64.7 nM
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CP0409410
3,4,5-trimethoxy-N-[N'-[4-methyl-3-[(4-phenylbenzoyl)amino]phenyl]carbamimidoyl]benzamide
   Show/Hide
C31H30N4O5
 1
1
IC50 = 5 nM
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CP0068085
2-chloro-N-(4-chloro-3-(pyridin-2-yl)phenyl)-4-(methylsulfonyl)benzamide
   Show/Hide
C19H14Cl2N2O3S
 2
1
IC50 = 5.1 nM
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2
IC50 = 7 nM
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CP0873343
(S)-N-(1-(3-fluorophenyl)-1H-pyrazol-5-yl)-1,3-dioxo-2-((1S,2R)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C25H23FN6O3
 2
1
IC50 = 7 nM
   TI
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2
IC50 = 9 nM
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CP0125962
4-((3-(2-fluoro-6-methoxyphenoxy)azetidin-1-yl)methyl)-5-methyl-1-phenyl-1H-pyrazole
   Show/Hide
C21H22FN3O2
 2
1
IC50 = 8.27 nM
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2
IC50 = 124 nM
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CP0416678
1-((5-chloro-3-methyl-1-phenyl-1H-pyrazol-4-yl)methyl)-4-(4-fluorophenyl)piperazine
   Show/Hide
C21H22ClFN4
 2
1
IC50 = 10 nM
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2
IC50 = 1860 nM
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CP0887656
(S)-1,3-dioxo-2-((1S,2R)-2-phenylcyclopropyl)-N-(2-phenylpyridin-3-yl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C27H25N5O3
 1
1
IC50 = 12 nM
   TI
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CP0282016
(8aS)-8a-methyl-1,3-dioxo-2-[(1S,2R)-2-phenylcyclopropyl]-N-(2-phenylpyrazol-3-yl)-6,8-dihydro-5H-imidazo[1,5-a]pyrazine-7-carboxamide
   Show/Hide
C26H26N6O3
 2
1
IC50 = 13 nM
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2
IC50 = 22 nM
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CP0851877
(S)-1,3-dioxo-N-(1-phenyl-1H-pyrazol-5-yl)-2-((1S,2R)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C25H24N6O3
 2
1
IC50 = 15 nM
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2
IC50 = 18 nM
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CP0917328
(S)-N-(2-(3-cyanophenyl)pyridin-3-yl)-1,3-dioxo-2-((1S,2R)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C28H24N6O3
 2
1
IC50 = 16 nM
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2
IC50 = 22 nM
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CP0873347
(S)-N-(1-(3-chlorophenyl)-1H-pyrazol-5-yl)-1,3-dioxo-2-((1S,2R)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C25H23ClN6O3
 2
1
IC50 = 18 nM
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2
IC50 = 32 nM
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CP0966119
(5-(4-chloro-3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)(morpholino)methanone
   Show/Hide
C26H25ClN4O2S
 1
1
IC50 = 18 nM
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CP0501233
(8aS)-8a-methyl-1,3-dioxo-2-[(1S,2R)-2-phenylcyclopropyl]-N-(2-phenylphenyl)-6,8-dihydro-5H-imidazo[1,5-a]pyrazine-7-carboxamide
   Show/Hide
C29H28N4O3
 2
1
IC50 = 19 nM
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2
IC50 = 60 nM
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CP0375517
1-((1-(4-fluorophenyl)-3,5-dimethyl-1H-pyrazol-4-yl)methyl)-4-(3-methoxyphenyl)piperazine
   Show/Hide
C23H27FN4O
 2
1
IC50 = 20 nM
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2
IC50 = 823 nM
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CP0655728
(S)-N-(biphenyl-2-yl)-1,3-dioxo-2-((1S,2R)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C28H26N4O3
 2
1
IC50 = 21 nM
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2
IC50 = 70 nM
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CP0910633
(S)-1,3-dioxo-2-((1S,2R)-2-phenylcyclopropyl)-N-(2-(thiophen-3-yl)pyridin-3-yl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C25H23N5O3S
 2
1
IC50 = 21 nM
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2
IC50 = 42 nM
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CP0416561
3,4,5-trimethoxy-N-[[4-methyl-3-[(4-phenylbenzoyl)amino]phenyl]carbamoyl]benzamide
   Show/Hide
C31H29N3O6
 1
1
IC50 = 24 nM
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CP0462652
N-[[1-(4-methoxyphenyl)indazol-5-yl]methyl]-2-propylpentanamide
   Show/Hide
C23H29N3O2
 1
1
IC50 = 40 nM
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CP0043416
4-((3-(2,5-dimethoxyphenyl)pyrrolidin-1-yl)methyl)-1-(2-fluorophenyl)-5-methyl-1H-pyrazole
   Show/Hide
C23H26FN3O2
 2
1
IC50 = 40.8 nM
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2
IC50 = 216 nM
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CP0851875
(S)-N-(2,6-dichloro-4-(methylsulfonyl)phenyl)-1,3-dioxo-2-((1S,2R)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C23H22Cl2N4O5S
 2
1
IC50 = 42 nM
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2
IC50 = 68 nM
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CP0967786
5-(4-chloro-3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-N-(pyridin-3-yl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridine-2-carboxamide
   Show/Hide
C27H22ClN5OS
 1
1
IC50 = 56 nM
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CP0416550
3,4,5-trimethoxy-N-[[4-methyl-3-[(4-phenylbenzoyl)amino]phenyl]carbamothioyl]benzamide
   Show/Hide
C31H29N3O5S
 1
1
IC50 = 63 nM
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CP0136263
(3S,3'R,3'aS,6'S,6aS,6bS,7'aR,9R,11aS,11bR)-3',6',10,11b-tetramethylspiro[2,3,4,6,6a,6b,7,8,11,11a-decahydro-1H-benzo[a]fluorene-9,2'-3a,4,5,6,7,7a-hexahydro-3H-furo[3,2-b]pyridine]-3-ol
   Show/Hide
C27H41NO2
 1
1
IC50 = 64 nM
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CP0496199
N-[[1-(3-methylphenyl)indazol-5-yl]methyl]-2-propylpentanamide
   Show/Hide
C23H29N3O
 1
1
IC50 = 70 nM
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CP0921973
(S)-N-(2,6-dichloro-4-(dimethylcarbamoyl)phenyl)-1,3-dioxo-2-((1S,2R)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C25H25Cl2N5O4
 2
1
IC50 = 77 nM
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2
IC50 = 120 nM
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CP0051889
1-((5-methyl-1-phenyl-1H-pyrazol-4-yl)methyl)-4-phenylpiperazine
   Show/Hide
C21H24N4
 2
1
IC50 = 78.4 nM
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2
IC50 = 96 nM
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CP0967785
(5-(4-chloro-3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)(4-(pyrimidin-2-yl)piperazin-1-yl)methanone
   Show/Hide
C30H28ClN7OS
 1
1
IC50 = 92 nM
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CP0462651
N-[[1-(3-methoxyphenyl)indazol-5-yl]methyl]-2-propylpentanamide
   Show/Hide
C23H29N3O2
 1
1
IC50 = 100 nM
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CP0884849
(S)-N-(2,6-dichloro-4-cyanophenyl)-1,3-dioxo-2-((1S,2R)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C23H19Cl2N5O3
 2
1
IC50 = 100 nM
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2
IC50 = 450 nM
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CP0453815
1-(4-methoxyphenyl)-4-((5-methyl-1-phenyl-1H-pyrazol-4-yl)methyl)piperazine
   Show/Hide
C22H26N4O
 2
1
IC50 = 105 nM
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2
IC50 = 126 nM
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CP0416536
N-[(3-benzamidophenyl)carbamoyl]-3,4,5-trimethoxybenzamide
   Show/Hide
C24H23N3O6
 1
1
IC50 = 107 nM
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CP0471969
N-[[1-(2,3-dimethylphenyl)indazol-5-yl]methyl]-2-propylpentanamide
   Show/Hide
C24H31N3O
 1
1
IC50 = 120 nM
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CP0429379
N-[[1-(6-methoxypyridin-3-yl)indazol-5-yl]methyl]-2-propylpentanamide
   Show/Hide
C22H28N4O2
 1
1
IC50 = 130 nM
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CP0472092
N-[[1-(4-fluorophenyl)indazol-5-yl]methyl]-2-propylpentanamide
   Show/Hide
C22H26FN3O
 1
1
IC50 = 130 nM
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CP0968344
(5-(4-chloro-3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)(4-methylpiperazin-1-yl)methanone
   Show/Hide
C27H28ClN5OS
 1
1
IC50 = 135 nM
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CP0505044
2-propyl-N-[[1-(5,6,7,8-tetrahydronaphthalen-1-yl)indazol-5-yl]methyl]pentanamide
   Show/Hide
C26H33N3O
 1
1
IC50 = 150 nM
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CP0851874
(S)-N-(2,6-dichloro-4-fluorophenyl)-1,3-dioxo-2-((1S,2R)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C22H19Cl2FN4O3
 2
1
IC50 = 180 nM
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2
IC50 = 520 nM
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CP0897580
(5-(4-chloro-3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)(4-hydroxypiperazin-1-yl)methanone
   Show/Hide
C27H27ClN4O2S
 1
1
IC50 = 192 nM
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CP0427252
N-[[1-(2-methylphenyl)indazol-5-yl]methyl]-2-propylpentanamide
   Show/Hide
C23H29N3O
 1
1
IC50 = 200 nM
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CP0521125
N-[(1-phenylindazol-5-yl)methyl]-2-propylpentanamide
   Show/Hide
C22H27N3O
 1
1
IC50 = 200 nM
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CP0952084
(5-(4-chloro-3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)(4-(3,4-dichlorophenyl)piperazin-1-yl)methanone
   Show/Hide
C32H28Cl3N5OS
 1
1
IC50 = 213 nM
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CP0968345
(5-(4-chloro-3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)(4-(methylsulfonyl)piperazin-1-yl)methanone
   Show/Hide
C27H28ClN5O3S2
 1
1
IC50 = 217 nM
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CP0884850
(S)-N-(2,3-dichlorophenyl)-1,3-dioxo-2-((1S,2R)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C22H20Cl2N4O3
 2
1
IC50 = 230 nM
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2
IC50 = 690 nM
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CP0894339
(5-(3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)(4-(pyrimidin-2-yl)piperazin-1-yl)methanone
   Show/Hide
C30H29N7OS
 1
1
IC50 = 237 nM
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CP0858770
5-(3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-N-(pyridin-3-yl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridine-2-carboxamide
   Show/Hide
C27H23N5OS
 1
1
IC50 = 261 nM
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CP0891470
(5-(3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)(morpholino)methanone
   Show/Hide
C26H26N4O2S
 1
1
IC50 = 294 nM
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CP0390282
N-[(3-benzamidophenyl)carbamothioyl]-3,4,5-trimethoxybenzamide
   Show/Hide
C24H23N3O5S
 1
1
IC50 = 300 nM
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CP0508050
3-[[1-(4-fluorophenyl)indazol-5-yl]methyl]-1,1-dipropylurea
   Show/Hide
C21H25FN4O
 1
1
IC50 = 310 nM
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CP0858771
(5-(4-chloro-3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)(pyrrolidin-1-yl)methanone
   Show/Hide
C26H25ClN4OS
 1
1
IC50 = 315 nM
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CP0952083
(5-(3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)(4-methylpiperazin-1-yl)methanone
   Show/Hide
C27H29N5OS
 1
1
IC50 = 326 nM
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CP0416679
1-((5-methyl-1-phenyl-1H-pyrazol-4-yl)methyl)-4-(3-(trifluoromethyl)phenyl)piperazine
   Show/Hide
C22H23F3N4
 2
1
IC50 = 328 nM
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2
IC50 = 333 nM
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CP0505995
N-[[1-(4-fluorophenyl)indazol-5-yl]methyl]-2,2-dimethylpentanamide
   Show/Hide
C21H24FN3O
 1
1
IC50 = 330 nM
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CP0462654
N-[[1-(2-methoxypyridin-3-yl)indazol-5-yl]methyl]-2-propylpentanamide
   Show/Hide
C22H28N4O2
 1
1
IC50 = 340 nM
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CP0963828
N,N-diethyl-5-(3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridine-2-carboxamide
   Show/Hide
C26H26N4OS
 1
1
IC50 = 352 nM
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CP0504283
N-[[1-(4-fluorophenyl)indazol-5-yl]methyl]-2-methylpentanamide
   Show/Hide
C20H22FN3O
 1
1
IC50 = 360 nM
   TI
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CP0967784
(5-(3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)(4-(methylsulfonyl)piperazin-1-yl)methanone
   Show/Hide
C27H29N5O3S2
 1
1
IC50 = 373 nM
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CP0966118
(4-hydroxypiperidin-1-yl)(5-(3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)methanone
   Show/Hide
C27H28N4O2S
 1
1
IC50 = 431 nM
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CP0968342
(4-(3,4-dichlorophenyl)piperazin-1-yl)(5-(3-(1-methyl-1H-benzo[d]imidazol-2-yl)phenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)methanone
   Show/Hide
C32H29Cl2N5OS
 1
1
IC50 = 456 nM
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CP0917084
(R)-N-(2,3-dichlorophenyl)-1,3-dioxo-2-((1S,2R)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
   Show/Hide
C22H20Cl2N4O3
 1
1
IC50 = 650 nM
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CP0917327
(S)-N-(2,3-dichlorophenyl)-1,3-dioxo-2-((1R,2S)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
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C22H20Cl2N4O3
 1
1
IC50 = 670 nM
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CP0514395
N-[[1-(3-fluorophenyl)indazol-5-yl]methyl]-2-propylpentanamide
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C22H26FN3O
 1
1
IC50 = 680 nM
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CP0514448
2-ethyl-N-[[1-(4-fluorophenyl)indazol-5-yl]methyl]butanamide
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C20H22FN3O
 1
1
IC50 = 720 nM
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CP0514371
N-[[1-(4-methylphenyl)indazol-5-yl]methyl]-2-propylpentanamide
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C23H29N3O
 1
1
IC50 = 900 nM
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CP0514663
N-[[1-(2-fluorophenyl)indazol-5-yl]methyl]-2-propylpentanamide
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C22H26FN3O
 1
1
IC50 = 900 nM
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CP0508051
N-[[1-(2,6-dimethylphenyl)indazol-5-yl]methyl]-2-propylpentanamide
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C24H31N3O
 1
1
IC50 = 950 nM
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CP0472093
N-[[1-(4-fluorophenyl)indazol-5-yl]methyl]pentanamide
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C19H20FN3O
 1
1
IC50 = 1200 nM
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CP0504285
N-[[1-(4-chlorophenyl)indazol-5-yl]methyl]-2-propylpentanamide
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C22H26ClN3O
 1
1
IC50 = 1200 nM
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CP0388253
(8aR)-8a-methyl-1,3-dioxo-2-[(1S,2R)-2-phenylcyclopropyl]-N-(2-phenylphenyl)-6,8-dihydro-5H-imidazo[1,5-a]pyrazine-7-carboxamide
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C29H28N4O3
 2
1
IC50 = 1200 nM
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2
IC50 = 1600 nM
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CP0505034
1-[[1-(4-fluorophenyl)indazol-5-yl]methyl]-1-methyl-3,3-dipropylurea
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C22H27FN4O
 1
1
IC50 = 1300 nM
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CP0462653
N-[[1-(4-acetylphenyl)indazol-5-yl]methyl]-2-propylpentanamide
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C24H29N3O2
 1
1
IC50 = 1400 nM
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CP0473611
1-((1-(4-fluorophenyl)-3,5-dimethyl-1H-pyrazol-4-yl)methyl)-4-(4-methoxyphenyl)piperazine
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C23H27FN4O
 2
1
IC50 = 1690 nM
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2
IC50 = 2000 nM
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CP0851876
(R)-N-(2,3-dichlorophenyl)-1,3-dioxo-2-((1R,2S)-2-phenylcyclopropyl)hexahydroimidazo[1,5-a]pyrazine-7(1H)-carboxamide
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C22H20Cl2N4O3
 1
1
IC50 = 1700 nM
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CP0402634
tert-butyl 4-[5-[(2-propylpentanoylamino)methyl]indazol-1-yl]benzoate
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C27H35N3O3
 1
1
IC50 = 1800 nM
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CP0514664
2-propyl-N-[(1-pyridin-3-ylindazol-5-yl)methyl]pentanamide
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C21H26N4O
 1
1
IC50 = 1830 nM
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CP0416673
1-((3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)methyl)-4-(2-methoxyphenyl)piperazine
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C23H28N4O
 2
1
IC50 = 1870 nM
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2
IC50 > 5000 nM
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CP0402633
N-[[1-(4-fluorophenyl)indazol-5-yl]methyl]-N-methyl-2-propylpentanamide
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C23H28FN3O
 1
1
IC50 = 3000 nM
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CP0455258
2-propyl-N-[(1-pyridin-4-ylindazol-5-yl)methyl]pentanamide
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C21H26N4O
 1
1
IC50 = 4500 nM
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CP0402635
2-propyl-N-[(1-pyrimidin-2-ylindazol-5-yl)methyl]pentanamide
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C20H25N5O
 1
1
IC50 = 6300 nM
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CP0427346
N-[[1-(4-fluorophenyl)indazol-5-yl]methyl]-2-phenylacetamide
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C22H18FN3O
 1
1
IC50 > 10000 nM
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CP0431076
N-[[1-(4-fluorophenyl)indazol-5-yl]methyl]cyclohexanecarboxamide
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C21H22FN3O
 1
1
IC50 = 10000 nM
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CP0455257
2-propyl-N-[(1-pyridin-2-ylindazol-5-yl)methyl]pentanamide
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C21H26N4O
 1
1
IC50 > 10000 nM
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CP0496436
N-[[1-(4-fluorophenyl)indazol-5-yl]methyl]-2-methylpropanamide
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C18H18FN3O
 1
1
IC50 = 10000 nM
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CP0499036
N-[[1-(4-fluorophenyl)indazol-5-yl]methyl]-2,2-dimethylpropanamide
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C19H20FN3O
 1
1
IC50 = 10000 nM
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CP0506002
N-[[1-(4-fluorophenyl)indazol-5-yl]methyl]benzamide
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C21H16FN3O
 1
1
IC50 = 10000 nM
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CP0504286
2-propyl-N-[(1-pyrazin-2-ylindazol-5-yl)methyl]pentanamide
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C20H25N5O
 1
1
IC50 > 25000 nM
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CP0416675
1-(4-methoxyphenyl)-4-((3-methyl-1-phenyl-1H-pyrazol-4-yl)methyl)piperazine
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C22H26N4O
 1
1
IC50 > 5000 nM
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CP0453814
1-(4-fluorophenyl)-4-((3-methyl-1-phenyl-1H-pyrazol-4-yl)methyl)piperazine
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C21H23FN4
 1
1
IC50 > 5000 nM
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