General Information of the Protein
Protein ID
PT04730
Protein Name
Histamine H4 receptor
Gene Name
HRH4
Sequence
MSESNSTGILPPAAQVPLAFLMSSFAFAIMVGNAVVILAFVVDRNLRHRSNYFFLNLAISDFLVGLISIPLYIPHVLFNWNFGSGICMFWLITDYLLCTASVYNIVLISYDRYQSVSNAVSYRAQHTGIMKIVAQMVAVWILAFLVNGPMILASDSWKNSTNTKDCEPGFVTEWYILTITMLLEFLLPVISVAYFNVQIYWSLWKRRALSRCPSHAGFSTTSSSASGHLHRAGVACRTSNPGLKESAASRHSESPRRKSSILVSLRTHMNSSITAFKVGSFWRSESAALRQREYAELLRGRKLARSLAILLSAFAICWAPYCLFTIVLSTYPRTERPKSVWYSIAFWLQWFNSFVNPFLYPLCHRRFQKAFWKILCVTKQPALSQNQSVSS
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Organism
Mus musculus, Mouse
Protein Classification
Membrane receptor
>
Family A G protein-coupled receptor
>
Small molecule receptor (family A GPCR)
>
Monoamine receptor
>
Histamine receptor
Function
The H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist) (By similarity).
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Uniprot ID
Q91ZY2
Ensembl ID
ENSG00000134489
Subcellular Location
Cell membrane
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000006 , HEK293
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0041262
 Compound Info 
5-chloro-2-[(4-methylpiperazin-1-yl)carbonyl]-1H-indole
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C14H16ClN3O
  1
1
EC50 = 2.399 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0173066
 Compound Info 
2,4-diamino-5,6-disubstituted pyrimidine, 11
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C18H23N5
  1
1
EC50 = 4.365 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0094765
 Compound Info 
14-fluoro-6-(piperazin-1-yl)-3,5-diazatricyclo[9.4.0.0^{2,7}]pentadeca-1(15),2(7),3,5,11,13-hexaen-4-amine
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C17H20FN5
  1
1
EC50 = 4.786 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0205085
 Compound Info 
2,4-diamino-5,6-disubstituted pyrimidine, 10
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C17H21N5
  1
1
EC50 = 5.754 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0173068
 Compound Info 
2,4-diamino-5,6-disubstituted pyrimidine, 12
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C17H21N5
  1
1
EC50 = 9.12 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0173069
 Compound Info 
5-chloro-2-(piperazin-1-ylcarbonyl)-1H-indole
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C13H14ClN3O
  1
1
EC50 = 17.78 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0198951
 Compound Info 
2,4-diamino-5,6-disubstituted pyrimidine, 9
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C17H21N5
  1
1
EC50 = 18.2 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0044460
 Compound Info 
N-cyclohexyl-4-(1H-imidazol-5-yl)piperidine-1-carbothioamide
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C15H24N4S
  1
1
EC50 = 28.18 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0229853
 Compound Info 
2,4-diamino-5,6-disubstituted pyrimidine, 14
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C19H25N5
  1
1
EC50 = 45.71 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0388279
 Compound Info 
4-[(1E,3E)-4-[1-[4-(1H-imidazol-5-yl)butyl]-2,6-dimethylpyridin-1-ium-4-yl]buta-1,3-dienyl]-N,N-dimethylaniline;2,2,2-trifluoroacetate;2,2,2-trifluoroacetic acid
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C30H34F6N4O4
  1
1
EC50 = 50.12 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0036920
 Compound Info 
2-(1H-imidazol-4-yl)ethan-1-amine
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C5H9N3
  1
1
EC50 = 94 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0286034
 Compound Info 
2-Aminopyrimidine analog., 25
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C15H16N6
  1
1
EC50 = 102.33 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0068173
 Compound Info 
2-Aminopyrimidine analog., 4
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C16H18N6
  1
1
EC50 = 123.03 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0382899
 Compound Info 
1-cyano-2-[[(1S,3S)-3-(1H-imidazol-5-yl)cyclopentyl]methyl]-3-(2-phenylsulfanylethyl)guanidine
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C19H24N6S
  1
1
EC50 = 166 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0377316
 Compound Info 
1-cyano-3-[[(1S,3S)-3-(1H-imidazol-5-yl)cyclopentyl]methyl]-2-methylguanidine
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C12H18N6
  1
1
EC50 = 538 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0377318
 Compound Info 
1-cyano-2-[[(1R,3R)-3-(1H-imidazol-5-yl)cyclopentyl]methyl]-3-(2-phenylsulfanylethyl)guanidine
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C19H24N6S
  1
1
EC50 = 589 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0823737
 Compound Info 
SID50106398
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C5H11Cl2N3
  1
1
EC50 = 2344.23 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0382908
 Compound Info 
1-cyano-3-[[(1R,3R)-3-(1H-imidazol-5-yl)cyclopentyl]methyl]-2-methylguanidine
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C12H18N6
  1
1
EC50 = 3631 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0224333
 Compound Info 
4-[(E)-2-(3-chlorophenyl)ethenyl]-6-(4-methylpiperazin-1-yl)-1,3,5-triazin-2-amine
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C16H19ClN6
  1
1
Ki = 854 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Cell Line ID: CL000025 , HEK-293T
 Cell Line Info 
Cell Line ID: CL000903 , HEK293-SF-hH4R-His6-CRE-Luc
 Cell Line Info 
Cell Line ID: CL000904 , HEK293-SF-mH4R-His6-CRE-Luc
 Cell Line Info 
Cell Line ID: CL000911 , HEK293T-beta-arr2-mH4R
 Cell Line Info 
Cell Line ID: CL000909 , HEK293T-SF-His6-CRE-Luc
 Cell Line Info 
Cell Line ID: CL000013 , Sf9
 Cell Line Info 
Cell Line ID: CL000074 , SK-N-MC
 Cell Line Info 
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0129069
 Compound Info 
4-cyclopentyl-6-piperazin-1-ylpyrimidin-2-amine
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C13H21N5
  1
1 EC50 = 7.3 nM
CP0213380
 Compound Info 
5-(3-(4-(5-fluoro-4-methyl-1H-benzo[d]imidazol-2-yl)-3-methylphenoxy)propyl)-4,5,6,7-tetrahydro-3H-imidazo[4,5-c]pyridine
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C24H26FN5O
  1
1 EC50 = 12 nM
CP0270460
 Compound Info 
3-[4-(5-fluoro-4-methyl-1H-benzimidazol-2-yl)-3-methylphenoxy]-N-[2-(1H-imidazol-5-yl)ethyl]propan-1-amine
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C23H26FN5O
  1
1 EC50 = 548 nM
CP0041262
 Compound Info 
5-chloro-2-[(4-methylpiperazin-1-yl)carbonyl]-1H-indole
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C14H16ClN3O
  1
1 EC50 = 794.33 nM
CP0041493
 Compound Info 
Clobenpropit
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C14H17ClN4S
  1
1 EC50 = 851.14 nM
CP0288244
 Compound Info 
5-fluoro-4-methyl-2-(2-methyl-4-(3-((4aR,7aR)-tetrahydro-1H-pyrrolo[3,4-b]pyridin-6(2H,7H,7aH)-yl)propoxy)phenyl)-1H-benzo[d]imidazole
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C25H31FN4O
  1
1 EC50 = 1197 nM
CP0036920
 Compound Info 
2-(1H-imidazol-4-yl)ethan-1-amine
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C5H9N3
  3
1 EC50 = 6760.83 nM
2 Kd = 42 nM
3 Ki = 42 nM
CP0058845
 Compound Info 
4-[(3R)-3-aminopyrrolidin-1-yl]-6-propan-2-ylpyrimidin-2-amine
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C11H19N5
  2
1 IC50 = 8 nM
2 Ki = 5.3 nM
CP0220828
 Compound Info 
4-N-(cyclopropylmethyl)-6-[(3R)-3-(methylamino)pyrrolidin-1-yl]pyrimidine-2,4-diamine
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C13H22N6
  1
1 Ki = 54.95 nM
Similar Protein(s) and Bioactivity Statistics
50% Identity
Protein ID Protein Name Protein Organism
PT00122 Histamine H4 receptor Cavia porcellus, Guinea pig
 Protein Info 
PT02519 Histamine H4 receptor Rattus norvegicus, Rat
 Protein Info