Protein Information

General Information of the Protein

Protein ID	PT03381
Protein Name	5'-nucleotidase
Secondarily Protein Name	5'-deoxynucleotidase Ecto-5'-nucleotidase IMP-specific 5'-nucleotidase Thymidylate 5'-phosphatase
Gene Name	NT5E
Secondarily Gene Name	NT5 NTE
Sequence	MCPRAARAPATLLLALGAVLWPAAGAWELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKFSTGSHCHGSFSLIFLSLWAVIFVLYQ Show/Hide
Organism	Homo sapiens, Human
Protein Classification	Enzyme > Phosphatase
Function	Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347). Show/Hide
Uniprot ID	Primary ID: P21589 Secondarily ID: B3KQI8 O75520 Q5W116 Show/Hide
Ensembl ID	ENSG00000135318
HGNC ID	HGNC:8021
Subcellular Location	Cell membrane

Map of Molecular Bioactivity Related to the Protein

Map of Molecular Bioactivity Related to the Protein Protein Cell Line Compound Bioactivity Value: <= 0.1 μM > 0.1 μM and <= 10 μM > 10 μM Imprecise Activity

Table of Molecular Bioactivities Related to the Protein

Cell Line ID: CL000053 , COS-7

Cell Line Info

Compound ID	Compound Name	Compound Formula
CP0953071 Compound Info	6'-amino-1-methyl-2-oxo-5-(trifluoromethoxy)-2',7'-dihydrospiro[indoline-3,4'-pyrazolo[3,4-b]pyridine]-5'-carbonitrile Show/Hide	C16H11F3N6O2	1
1	IC50 = 150 nM Bioactivity Info	TI LI LO TS
CP0962772 Compound Info	6'-amino-1,3'-dimethyl-2-oxo-5-(trifluoromethoxy)-2',7'-dihydrospiro[indoline-3,4'-pyrazolo[3,4-b]pyridine]-5'-carbonitrile Show/Hide	C17H13F3N6O2	1
1	IC50 = 180 nM Bioactivity Info	TI LI LO TS
CP0955878 Compound Info	6'-amino-5-fluoro-1,3'-dimethyl-2-oxo-2',7'-dihydrospiro[indoline-3,4'-pyrazolo[3,4-b]pyridine]-5'-carbonitrile Show/Hide	C16H13FN6O	1
1	IC50 = 260 nM Bioactivity Info	TI LI LO TS
CP0182233 Compound Info	3-[[(Z)-(4-oxo-2-propan-2-yloxychromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C19H20N2O5S	2
1	IC50 = 287 nM Bioactivity Info	TI LI LO TS
2	Ki = 14 nM Bioactivity Info	TI LI LO TS
CP0511978 Compound Info	2-[5-chloro-3-methyl-1-(4-methylphenyl)pyrazolo[3,4-b]pyridin-6-yl]-4-methylphenol Show/Hide	C21H18ClN3O	1
1	IC50 = 320 nM Bioactivity Info	TI LI LO TS
CP0095967 Compound Info	3-[[(Z)-(2-butoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C20H22N2O5S	2
1	IC50 = 377 nM Bioactivity Info	TI LI LO TS
2	Ki = 18 nM Bioactivity Info	TI LI LO TS
CP0182234 Compound Info	3-[[(Z)-(2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H16N2O5S	2
1	IC50 = 380 nM Bioactivity Info	TI LI LO TS
2	Ki = 18 nM Bioactivity Info	TI LI LO TS
CP0953070 Compound Info	6'-amino-5-fluoro-1-methyl-2-oxo-2',7'-dihydrospiro[indoline-3,4'-pyrazolo[3,4-b]pyridine]-5'-carbonitrile Show/Hide	C15H11FN6O	1
1	IC50 = 460 nM Bioactivity Info	TI LI LO TS
CP0060026 Compound Info	3-[[(Z)-(6-chloro-2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H15ClN2O5S	2
1	IC50 = 560 nM Bioactivity Info	TI LI LO TS
2	Ki = 27 nM Bioactivity Info	TI LI LO TS
CP0911315 Compound Info	6'-amino-1-methyl-2-oxo-2',7'-dihydrospiro[indoline-3,4'-pyrazolo[3,4-b]pyridine]-5'-carbonitrile Show/Hide	C15H12N6O	1
1	IC50 = 690 nM Bioactivity Info	TI LI LO TS
CP0046660 Compound Info	2-[[(Z)-(6,8-dichloro-2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H14Cl2N2O5S	2
1	IC50 = 880 nM Bioactivity Info	TI LI LO TS
2	Ki = 42 nM Bioactivity Info	TI LI LO TS
CP0091335 Compound Info	3-[[(Z)-(6,8-dichloro-2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H14Cl2N2O5S	2
1	IC50 = 970 nM Bioactivity Info	TI LI LO TS
2	Ki = 46 nM Bioactivity Info	TI LI LO TS
CP0054925 Compound Info	4-[[(Z)-(4-oxo-2-propan-2-yloxychromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C19H20N2O5S	2
1	IC50 = 1170 nM Bioactivity Info	TI LI LO TS
2	Ki = 53 nM Bioactivity Info	TI LI LO TS
CP0563724 Compound Info	6-amino-4-hydroxynaphthalene-2-sulfonic acid Show/Hide	C10H9NO4S	1
1	IC50 = 1320 nM Bioactivity Info	TI LI LO TS
CP0112915 Compound Info	4-[[(Z)-(2-butoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C20H22N2O5S	2
1	IC50 = 1320 nM Bioactivity Info	TI LI LO TS
2	Ki = 63 nM Bioactivity Info	TI LI LO TS
CP0684278 Compound Info	(rac)-2-{[(Z)-(6-chloro-2-methoxy-4-oxo-2H-chromen-3(4H)-ylidene)methyl]amino}benzenesulfonamide Show/Hide	C17H15ClN2O5S	2
1	IC50 = 1350 nM Bioactivity Info	TI LI LO TS
2	Ki = 64 nM Bioactivity Info	TI LI LO TS
CP0074437 Compound Info	4-[[(Z)-(6-chloro-2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H15ClN2O5S	2
1	IC50 = 1660 nM Bioactivity Info	TI LI LO TS
2	Ki = 79 nM Bioactivity Info	TI LI LO TS
CP0095966 Compound Info	2-[[(Z)-(2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H16N2O5S	2
1	IC50 = 1910 nM Bioactivity Info	TI LI LO TS
2	Ki = 91 nM Bioactivity Info	TI LI LO TS
CP0098449 Compound Info	4-[[(Z)-(6,8-dichloro-2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H14Cl2N2O5S	2
1	IC50 = 2920 nM Bioactivity Info	TI LI LO TS
2	Ki = 138 nM Bioactivity Info	TI LI LO TS
CP0853552 Compound Info	4-(3-methyl-5-oxo-4,5-dihydropyrazol-1-yl)benzenesulfonic acid Show/Hide	C10H10N2O4S	1
1	IC50 = 6100 nM Bioactivity Info	TI LI LO TS
CP0095965 Compound Info	4-[[(Z)-(2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H16N2O5S	2
1	IC50 = 7110 nM Bioactivity Info	TI LI LO TS
2	Ki = 338 nM Bioactivity Info	TI LI LO TS
CP0444253 Compound Info	CHEBI:27500 Show/Hide	C6H7NO3S	1
1	IC50 = 8200 nM Bioactivity Info	TI LI LO TS
CP0042258 Compound Info	2-(4-aminophenyl)-6-methyl-1,3-benzothiazole-7-sulfonic acid Show/Hide	C14H12N2O3S2	1
1	IC50 = 13500 nM Bioactivity Info	TI LI LO TS
CP0441935 Compound Info	4-Amino-5-hydroxy-naphthalene-2,7-disulfonic acid; sodium salt Show/Hide	C10H9NO7S2	1
1	IC50 = 21800 nM Bioactivity Info	TI LI LO TS
CP0386951 Compound Info	6-(carbamoylamino)-4-hydroxynaphthalene-2-sulfonic acid Show/Hide	C11H10N2O5S	1
1	IC50 = 30500 nM Bioactivity Info	TI LI LO TS
CP0403596 Compound Info	4-amino-3-hydroxy-7-nitronaphthalene-1-sulfonic acid Show/Hide	C10H8N2O6S	1
1	IC50 = 41000 nM Bioactivity Info	TI LI LO TS
CP0037056 Compound Info	dihydroxy-imino-oxo-lambda6-sulfane Show/Hide	H3NO3S	1
1	IC50 = 42100 nM Bioactivity Info	TI LI LO TS
CP0479789 Compound Info	5-amino-2-(4-nitroanilino)benzenesulfonic acid Show/Hide	C12H11N3O5S	1
1	IC50 = 47600 nM Bioactivity Info	TI LI LO TS

Cell Line ID: CL000011 , CHO

Cell Line Info

Cell Line ID: CL000111 , MDA-MB-231

Cell Line Info

Cell Line ID: CL000562 , My-La CD8+

Cell Line Info

Cell Line ID: CL000563 , NCI-H1568

Cell Line Info

Cell Line ID: CL000013 , Sf9

Cell Line Info

Cell Line ID: CL000084 , SK-OV-3

Cell Line Info

Biochemical Assays

Compound ID	Compound Name	Compound Formula
CP0135378 Compound Info	[[(2R,3S,4R,5R)-5-[6-chloro-4-[[(1S)-1-(2-fluorophenyl)ethyl]amino]pyrazolo[3,4-b]pyridin-1-yl]-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]methylphosphonic acid Show/Hide	C20H24ClFN4O9P2	1
1	IC50 = 0.043 nM
CP0421424 Compound Info	[[(2R,3S,4R,5R)-5-[6-chloro-4-[[(1S)-1-phenylethyl]amino]pyrazolo[3,4-d]pyrimidin-1-yl]-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]methylphosphonic acid Show/Hide	C19H24ClN5O9P2	1
1	IC50 = 0.053 nM
CP0472500 Compound Info	[(2R,3S,4R,5R)-5-[6-chloro-4-(cyclopentylamino)pyrazolo[3,4-d]pyrimidin-1-yl]-3,4-dihydroxyoxolan-2-yl]methoxymethylphosphonic acid Show/Hide	C16H23ClN5O7P	1
1	IC50 = 0.86 nM
CP0136183 Compound Info	ADP, alpha beta-me Show/Hide	C11H17N5O9P2	5
1	IC50 = 187 nM
2	IC50 = 1100 nM
3	IC50 = 2500 nM
4	IC50 = 3970 nM
5	Ki = 88.4 nM
CP0135870 Compound Info	[[(2R,3S,4R,5R)-5-[6-[benzyl(methyl)amino]-2-chloropurin-9-yl]-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]methylphosphonic acid Show/Hide	C19H24ClN5O9P2	1
1	Ki = 0.318 nM
CP0357536 Compound Info	[[(2R,3S,4R,5R)-5-(5-fluoro-2,4-dioxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]methylphosphonic acid Show/Hide	C10H15FN2O11P2	1
1	Ki = 2540 nM

Clinical Information about the Protein

Target 1 ( Ecto-5'-nucleotidase (CD73) )

Target Type	Clinical trial Target
Disease	1 Target-related Disease				1
1	Pancreatic cancer [ICD-11: 2C10]
Clinical Trial Drug(s)	1 Clinical Trial Drug				1
1	AB680	Drug Info	Phase 1	Pancreatic cancer

Similar Protein(s) and Bioactivity Statistics

50% Identity

Protein ID	Protein Name	Protein Organism
PT04712	5'-nucleotidase	Rattus norvegicus, Rat	Protein Info

Cite

About MolBiC

Correspondence

Prof. Feng ZHU

Prof. Leo, Lianyi Han