Protein Information

General Information of the Protein

Protein ID	PT04712
Protein Name	5'-nucleotidase
Secondarily Protein Name	5'-deoxynucleotidase Ecto-5'-nucleotidase IMP-specific 5'-nucleotidase Thymidylate 5'-phosphatase
Gene Name	NT5E
Secondarily Gene Name	Nt5 Nte
Sequence	MRPAAATAPKWLLLALSALLPLWPTAKSWELTIMHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLLLDAGDQYQGTIWFTVYKGLEVAHFMNLLGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAPQISGLYLPYKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEVTALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHTNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKVEFDDKGNVVTSYGNPILLNSTIREDAAIKADINQWRIKLDNYSTQELGRTIVYLNGSAQECRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDISRKPWDRVVQLKVLCTKCRVPIYEPLEMDKVYKVVLPSYLVNGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVIYPAVEGRIKFSAASHYQGSFPLIILSFWAVILVLYQ Show/Hide
Organism	Rattus norvegicus, Rat
Protein Classification	Enzyme > Phosphatase
Function	Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside (PubMed:1770988). AMP is the preferred substrate but can also hydrolyze CMP and GMP (PubMed:1770988). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (By similarity). Other substrates include IMP, UMP, dAMP, dCMP, dTMP, NAD and NMN (By similarity). Show/Hide
Uniprot ID	P21588
Ensembl ID	ENSG00000135318
Subcellular Location	Cell membrane

Map of Molecular Bioactivity Related to the Protein

Map of Molecular Bioactivity Related to the Protein Protein Cell Line Compound Bioactivity Value: <= 0.1 μM > 0.1 μM and <= 10 μM > 10 μM Imprecise Activity

Table of Molecular Bioactivities Related to the Protein

Cell Line ID: CL000053 , COS-7

Cell Line Info

Compound ID	Compound Name	Compound Formula
CP0839008 Compound Info	2-{[(6-Ethyl-2-ethoxy-4-oxo-2H-chromen-3(4H)-ylidene)methyl]amino}benzenesulfonamide Show/Hide	C20H22N2O5S	2
1	IC50 = 101 nM Bioactivity Info	TI LI LO TS
2	Ki = 4.7 nM Bioactivity Info	TI LI LO TS
CP0953072 Compound Info	6'-amino-1,3'-dimethyl-2-oxo-2',7'-dihydrospiro[indoline-3,4'-pyrazolo[3,4-b]pyridine]-5'-carbonitrile Show/Hide	C16H14N6O	1
1	IC50 = 190 nM Bioactivity Info	TI LI LO TS
CP0953071 Compound Info	6'-amino-1-methyl-2-oxo-5-(trifluoromethoxy)-2',7'-dihydrospiro[indoline-3,4'-pyrazolo[3,4-b]pyridine]-5'-carbonitrile Show/Hide	C16H11F3N6O2	1
1	IC50 = 520 nM Bioactivity Info	TI LI LO TS
CP0054925 Compound Info	4-[[(Z)-(4-oxo-2-propan-2-yloxychromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C19H20N2O5S	2
1	IC50 = 540 nM Bioactivity Info	TI LI LO TS
2	Ki = 26 nM Bioactivity Info	TI LI LO TS
CP0774137 Compound Info	3-{[(6,8-Dibromo-2-ethoxy-4-oxo-2H-chromen-3(4H)-ylidene)methyl]amino}benzenesulfonamide Show/Hide	C18H16Br2N2O5S	2
1	IC50 = 583 nM Bioactivity Info	TI LI LO TS
2	Ki = 27 nM Bioactivity Info	TI LI LO TS
CP0953066 Compound Info	6'-amino-5-chloro-1-methyl-2-oxo-2',7'-dihydrospiro[indoline-3,4'-pyrazolo[3,4-b]pyridine]-5'-carbonitrile Show/Hide	C15H11ClN6O	1
1	IC50 = 680 nM Bioactivity Info	TI LI LO TS
CP0674324 Compound Info	3-{[(6-Fluoro-2-ethoxy-4-oxo-2H-chromen-3(4H)-ylidene)methyl]amino}benzenesulfonamide Show/Hide	C18H17FN2O5S	2
1	IC50 = 990 nM Bioactivity Info	TI LI LO TS
2	Ki = 47 nM Bioactivity Info	TI LI LO TS
CP0399431 Compound Info	4-[[(Z)-(2-ethoxy-4-oxochromen-3-ylidene)methyl]amino]-N-(6-methoxypyridazin-3-yl)benzenesulfonamide Show/Hide	C23H22N4O6S	2
1	IC50 = 1090 nM Bioactivity Info	TI LI LO TS
2	Ki = 51.7 nM Bioactivity Info	TI LI LO TS
CP0684278 Compound Info	(rac)-2-{[(Z)-(6-chloro-2-methoxy-4-oxo-2H-chromen-3(4H)-ylidene)methyl]amino}benzenesulfonamide Show/Hide	C17H15ClN2O5S	2
1	IC50 = 1310 nM Bioactivity Info	TI LI LO TS
2	Ki = 62 nM Bioactivity Info	TI LI LO TS
CP0968051 Compound Info	6'-amino-5-chloro-1,3'-dimethyl-2-oxo-2',7'-dihydrospiro[indoline-3,4'-pyrazolo[3,4-b]pyridine]-5'-carbonitrile Show/Hide	C16H13ClN6O	1
1	IC50 = 1560 nM Bioactivity Info	TI LI LO TS
CP0074437 Compound Info	4-[[(Z)-(6-chloro-2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H15ClN2O5S	2
1	IC50 = 1710 nM Bioactivity Info	TI LI LO TS
2	Ki = 81 nM Bioactivity Info	TI LI LO TS
CP0046660 Compound Info	2-[[(Z)-(6,8-dichloro-2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H14Cl2N2O5S	2
1	IC50 = 1840 nM Bioactivity Info	TI LI LO TS
2	Ki = 87 nM Bioactivity Info	TI LI LO TS
CP0922745 Compound Info	6'-amino-1-methyl-5-nitro-2-oxo-2',7'-dihydrospiro[indoline-3,4'-pyrazolo[3,4-b]pyridine]-5'-carbonitrile Show/Hide	C15H11N7O3	1
1	IC50 = 2120 nM Bioactivity Info	TI LI LO TS
CP0659236 Compound Info	4-{[(2-Ethoxy-6-fluoro-4-oxo-2H-chromen-3(4H)-ylidene)methyl]amino}benzenesulfonamide Show/Hide	C18H17FN2O5S	2
1	IC50 = 2130 nM Bioactivity Info	TI LI LO TS
2	Ki = 101 nM Bioactivity Info	TI LI LO TS
CP0060026 Compound Info	3-[[(Z)-(6-chloro-2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H15ClN2O5S	2
1	IC50 = 2380 nM Bioactivity Info	TI LI LO TS
2	Ki = 113 nM Bioactivity Info	TI LI LO TS
CP0095966 Compound Info	2-[[(Z)-(2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H16N2O5S	2
1	IC50 = 2390 nM Bioactivity Info	TI LI LO TS
2	Ki = 114 nM Bioactivity Info	TI LI LO TS
CP0085957 Compound Info	3-(1,1-dioxo-3,4-dihydro-2H-1lambda6,2,4-benzothiadiazin-3-yl)-6-fluorochromen-4-one Show/Hide	C16H11FN2O4S	2
1	IC50 = 2710 nM Bioactivity Info	TI LI LO TS
2	Ki = 129 nM Bioactivity Info	TI LI LO TS
CP0182234 Compound Info	3-[[(Z)-(2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H16N2O5S	2
1	IC50 = 2810 nM Bioactivity Info	TI LI LO TS
2	Ki = 134 nM Bioactivity Info	TI LI LO TS
CP0962772 Compound Info	6'-amino-1,3'-dimethyl-2-oxo-5-(trifluoromethoxy)-2',7'-dihydrospiro[indoline-3,4'-pyrazolo[3,4-b]pyridine]-5'-carbonitrile Show/Hide	C17H13F3N6O2	1
1	IC50 = 3560 nM Bioactivity Info	TI LI LO TS
CP0095967 Compound Info	3-[[(Z)-(2-butoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C20H22N2O5S	2
1	IC50 = 3610 nM Bioactivity Info	TI LI LO TS
2	Ki = 172 nM Bioactivity Info	TI LI LO TS
CP0674325 Compound Info	4-{[(6-Ethyl-2-ethoxy-4-oxo-2H-chromen-3(4H)-ylidene)methyl]amino}benzenesulfonamide Show/Hide	C20H22N2O5S	2
1	IC50 = 3730 nM Bioactivity Info	TI LI LO TS
2	Ki = 178 nM Bioactivity Info	TI LI LO TS
CP0088299 Compound Info	3-(1,1-dioxo-3,4-dihydro-2H-1lambda6,2,4-benzothiadiazin-3-yl)chromen-4-one Show/Hide	C16H12N2O4S	2
1	IC50 = 3810 nM Bioactivity Info	TI LI LO TS
2	Ki = 181 nM Bioactivity Info	TI LI LO TS
CP0091335 Compound Info	3-[[(Z)-(6,8-dichloro-2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H14Cl2N2O5S	2
1	IC50 = 4810 nM Bioactivity Info	TI LI LO TS
2	Ki = 229 nM Bioactivity Info	TI LI LO TS
CP0955878 Compound Info	6'-amino-5-fluoro-1,3'-dimethyl-2-oxo-2',7'-dihydrospiro[indoline-3,4'-pyrazolo[3,4-b]pyridine]-5'-carbonitrile Show/Hide	C16H13FN6O	1
1	IC50 = 5010 nM Bioactivity Info	TI LI LO TS
CP0733612 Compound Info	3-{[(6-Bromo-2-ethoxy-4-oxo-2H-chromen-3(4H)-ylidene)methyl]amino}benzenesulfonamide Show/Hide	C18H17BrN2O5S	2
1	IC50 = 6130 nM Bioactivity Info	TI LI LO TS
2	Ki = 292 nM Bioactivity Info	TI LI LO TS
CP0953070 Compound Info	6'-amino-5-fluoro-1-methyl-2-oxo-2',7'-dihydrospiro[indoline-3,4'-pyrazolo[3,4-b]pyridine]-5'-carbonitrile Show/Hide	C15H11FN6O	1
1	IC50 = 7990 nM Bioactivity Info	TI LI LO TS
CP0098449 Compound Info	4-[[(Z)-(6,8-dichloro-2-methoxy-4-oxochromen-3-ylidene)methyl]amino]benzenesulfonamide Show/Hide	C17H14Cl2N2O5S	2
1	IC50 = 10100 nM Bioactivity Info	TI LI LO TS
2	Ki = 472 nM Bioactivity Info	TI LI LO TS
CP0563724 Compound Info	6-amino-4-hydroxynaphthalene-2-sulfonic acid Show/Hide	C10H9NO4S	1
1	IC50 = 10400 nM Bioactivity Info	TI LI LO TS
CP0385018 Compound Info	4-[[(Z)-(2-ethoxy-4-oxochromen-3-ylidene)methyl]amino]-N-pyridin-2-ylbenzenesulfonamide Show/Hide	C23H21N3O5S	2
1	IC50 = 15180 nM Bioactivity Info	TI LI LO TS
2	Ki = 712 nM Bioactivity Info	TI LI LO TS
CP0042258 Compound Info	2-(4-aminophenyl)-6-methyl-1,3-benzothiazole-7-sulfonic acid Show/Hide	C14H12N2O3S2	1
1	IC50 = 18600 nM Bioactivity Info	TI LI LO TS
CP0403596 Compound Info	4-amino-3-hydroxy-7-nitronaphthalene-1-sulfonic acid Show/Hide	C10H8N2O6S	1
1	IC50 = 31600 nM Bioactivity Info	TI LI LO TS
CP0673256 Compound Info	2-{[(6,8-Dibromo-2-ethoxy-4-oxo-2H-chromen-3(4H)-ylidene)methyl]amino}benzenesulfonamide Show/Hide	C18H16Br2N2O5S	2
1	IC50 = 40100 nM Bioactivity Info	TI LI LO TS
2	Ki = 1910 nM Bioactivity Info	TI LI LO TS
CP0853552 Compound Info	4-(3-methyl-5-oxo-4,5-dihydropyrazol-1-yl)benzenesulfonic acid Show/Hide	C10H10N2O4S	1
1	IC50 = 44300 nM Bioactivity Info	TI LI LO TS
CP0444253 Compound Info	CHEBI:27500 Show/Hide	C6H7NO3S	1
1	IC50 = 70100 nM Bioactivity Info	TI LI LO TS
CP0479789 Compound Info	5-amino-2-(4-nitroanilino)benzenesulfonic acid Show/Hide	C12H11N3O5S	1
1	IC50 = 72900 nM Bioactivity Info	TI LI LO TS
CP0441935 Compound Info	4-Amino-5-hydroxy-naphthalene-2,7-disulfonic acid; sodium salt Show/Hide	C10H9NO7S2	1
1	IC50 = 76600 nM Bioactivity Info	TI LI LO TS
CP0037056 Compound Info	dihydroxy-imino-oxo-lambda6-sulfane Show/Hide	H3NO3S	1
1	IC50 = 77300 nM Bioactivity Info	TI LI LO TS

Cell Line ID: CL000013 , Sf9

Cell Line Info

Biochemical Assays

Compound ID	Compound Name	Compound Formula
CP0135870 Compound Info	[[(2R,3S,4R,5R)-5-[6-[benzyl(methyl)amino]-2-chloropurin-9-yl]-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]methylphosphonic acid Show/Hide	C19H24ClN5O9P2	1
1	Ki = 0.746 nM

Similar Protein(s) and Bioactivity Statistics

50% Identity

Protein ID	Protein Name	Protein Organism
PT03381	5'-nucleotidase	Homo sapiens, Human	Protein Info

Cite

About MolBiC

Correspondence

Prof. Feng ZHU

Prof. Leo, Lianyi Han