General Information of the Protein
Protein ID
PT01856
Protein Name
Nitric oxide synthase 3
Secondarily
Protein Name
Constitutive NOS
EC-NOS
NOS type III
Nitric oxide synthase
endothelial
Gene Name
NOS3
Sequence
MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP
    Show/Hide
Organism
Homo sapiens, Human
Protein Classification
Enzyme
Function
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Show/Hide
Uniprot ID
Primary ID:
P29474

Secondarily ID:
A0S0A7
A0S0A8
A8KA63
B2RCQ1
E9PFR2
Q13662
Q14251
Q14434
Q548C1
Q6GSL5
Q9UDC6
    Show/Hide
Ensembl ID
ENSG00000164867
HGNC ID
HGNC:7876
Subcellular Location
Cell membrane
Membrane
Caveola
Cytoplasm
Cytoskeleton
Golgi apparatus
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000749 , HUVEC-C
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0376504
 Compound Info 
5-Cyclopropyl-4,5-dihydro-thieno[2,3-c]pyridin-7-ylamine
   Show/Hide
C10H12N2S
  1
1
IC50 = 50 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0425444
 Compound Info 
5-Ethynyl-4,5-dihydro-thieno[2,3-c]pyridin-7-ylamine
   Show/Hide
C9H8N2S
  1
1
IC50 = 80 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0536715
 Compound Info 
6-Ethynyl-6,7-dihydro-thieno[3,2-c]pyridin-4-ylamine
   Show/Hide
C9H8N2S
  1
1
IC50 = 120 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0506680
 Compound Info 
8-Fluoro-3-(4-fluoro-phenyl)-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C15H12F2N2
  1
1
IC50 = 160 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0454759
 Compound Info 
4,5-Dihydro-thieno[2,3-c]pyridin-7-ylamine
   Show/Hide
C7H8N2S
  1
1
IC50 = 280 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0053263
 Compound Info 
(2S)-2-azaniumyl-5-[(N'-methylcarbamimidoyl)amino]pentanoate
   Show/Hide
C7H16N4O2
  1
1
IC50 = 300 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0383554
 Compound Info 
6-Cyclopropyl-6,7-dihydro-thieno[3,2-c]pyridin-4-ylamine
   Show/Hide
C10H12N2S
  1
1
IC50 = 750 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0507314
 Compound Info 
3-Ethynyl-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C11H10N2
  1
1
IC50 = 900 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0380179
 Compound Info 
5-Furan-2-yl-4,5-dihydro-thieno[2,3-c]pyridin-7-ylamine
   Show/Hide
C11H10N2OS
  1
1
IC50 = 920 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0402943
 Compound Info 
5-Thiophen-2-yl-4,5-dihydro-thieno[2,3-c]pyridin-7-ylamine
   Show/Hide
C11H10N2S2
  1
1
IC50 = 1900 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0464414
 Compound Info 
3,4-Dihydro-isoquinolin-1-ylamine
   Show/Hide
C9H10N2
  1
1
IC50 = 2000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0044041
 Compound Info 
(L-N6-1-iminoethyl)lysine
   Show/Hide
C8H17N3O2
  1
1
IC50 = 2400 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0043950
 Compound Info 
(S)-methyl 2-amino-5-(amino(nitroamino)methyleneamino)pentanoate
   Show/Hide
C7H15N5O4
  1
1
IC50 = 2700 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0375487
 Compound Info 
3-Cyclopropyl-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C12H14N2
  1
1
IC50 = 3600 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0463597
 Compound Info 
3-Methyl-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C10H12N2
  1
1
IC50 = 4000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0383447
 Compound Info 
5-Phenyl-4,5-dihydro-thieno[2,3-c]pyridin-7-ylamine
   Show/Hide
C13H12N2S
  1
1
IC50 = 4000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0466769
 Compound Info 
8-Chloro-3-(4-fluoro-phenyl)-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C15H12ClFN2
  1
1
IC50 = 5000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0565395
 Compound Info 
3-Furan-2-yl-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C13H12N2O
  1
1
IC50 = 5000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0463500
 Compound Info 
5-Prop-1-ynyl-4,5-dihydro-thieno[2,3-c]pyridin-7-ylamine
   Show/Hide
C10H10N2S
  1
1
IC50 = 5000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0540144
 Compound Info 
8-Chloro-3-phenyl-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C15H13ClN2
  1
1
IC50 = 6000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0439003
 Compound Info 
3-Thiophen-2-yl-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C13H12N2S
  1
1
IC50 = 8000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0375511
 Compound Info 
3-Phenyl-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C15H14N2
  1
1
IC50 = 9000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0380411
 Compound Info 
3-(3-Fluoro-phenyl)-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C15H13FN2
  1
1
IC50 = 9000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0466341
 Compound Info 
6-Phenyl-6,7-dihydro-thieno[3,2-c]pyridin-4-ylamine
   Show/Hide
C13H12N2S
  1
1
IC50 = 9500 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0437466
 Compound Info 
5-Ethynyl-2-methyl-4,5-dihydro-thieno[2,3-c]pyridin-7-ylamine
   Show/Hide
C10H10N2S
  1
1
IC50 = 10000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0533020
 Compound Info 
4,5-Dihydro-3H-benzo[c]azepin-1-ylamine
   Show/Hide
C10H12N2
  1
1
IC50 = 12000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0416781
 Compound Info 
3-Thiazol-2-yl-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C12H11N3S
  1
1
IC50 = 19000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0403965
 Compound Info 
3-(4-Fluoro-phenyl)-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C15H13FN2
  1
1
IC50 = 40000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0496468
 Compound Info 
3-(2-Fluoro-phenyl)-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C15H13FN2
  1
1
IC50 = 44000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0518313
 Compound Info 
5-Fluoro-3-(4-fluoro-phenyl)-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C15H12F2N2
  1
1
IC50 = 44000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0503502
 Compound Info 
7-Fluoro-3-(4-fluoro-phenyl)-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C15H12F2N2
  1
1
IC50 = 55000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0055850
 Compound Info 
Aminoguanidine
   Show/Hide
CH6N4
  1
1
IC50 = 72000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0466928
 Compound Info 
8-Fluoro-3-phenyl-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C15H13FN2
  1
1
IC50 = 80000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0492145
 Compound Info 
3-(4-Chloro-phenyl)-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C15H13ClN2
  1
1
IC50 = 86000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0404434
 Compound Info 
6-Fluoro-3-(4-fluoro-phenyl)-3,4-dihydro-isoquinolin-1-ylamine
   Show/Hide
C15H12F2N2
  1
1
IC50 = 100000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Cell Line ID: CL000006 , HEK293
 Cell Line Info 
Cell Line ID: CL000012 , Sf21
 Cell Line Info 
Cell Line ID: CL000013 , Sf9
 Cell Line Info 
Cell Line ID: CL000040 , THP-1
 Cell Line Info 
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0053263
 Compound Info 
(2S)-2-azaniumyl-5-[(N'-methylcarbamimidoyl)amino]pentanoate
   Show/Hide
C7H16N4O2
  7
1 IC50 = 540 nM
2 IC50 = 650 nM
3 IC50 = 3500 nM
4 IC50 = 5900 nM
5 IC50 = 6000 nM
6 IC50 = 8700 nM
7 Ki = 400 nM
CP0043950
 Compound Info 
(S)-methyl 2-amino-5-(amino(nitroamino)methyleneamino)pentanoate
   Show/Hide
C7H15N5O4
  2
1 IC50 = 1500 nM
2 IC50 = 2700 nM
CP0047550
 Compound Info 
7-nitro-1H-indazole
   Show/Hide
C7H5N3O2
  2
1 IC50 = 1890 nM
2 IC50 = 2500 nM
CP0044041
 Compound Info 
(L-N6-1-iminoethyl)lysine
   Show/Hide
C8H17N3O2
  5
1 IC50 = 2400 nM
2 IC50 = 2600 nM
3 IC50 = 7900 nM
4 IC50 = 38000 nM
5 IC50 > 100000 nM
CP0223802
 Compound Info 
N'-[[3-(aminomethyl)phenyl]methyl]ethanimidamide
   Show/Hide
C10H15N3
  1
1 IC50 = 51200 nM
CP0055850
 Compound Info 
Aminoguanidine
   Show/Hide
CH6N4
  1
1 IC50 = 100000 nM
CP0040036
 Compound Info 
(S)-5-Acetimidoylamino-2-amino-pent
   Show/Hide
C7H15N3O2
  1
1 Ki = 810 nM
Clinical Information about the Protein
Target 1 ( Nitric-oxide synthase endothelial (NOS3) )
Target Type Clinical trial Target
Disease 3 Target-related Diseases  3
1 Coronary artery disease [ICD-11: BA80]
2 Hypertension [ICD-11: BA00-BA04]
3 Myocardial infarction [ICD-11: BA41-BA43]
Clinical Trial Drug(s) 3 Clinical Trial Drugs  3
1 ACCLAIM
 Drug Info 
Phase 2
Coronary artery disease
2 L-NAME
 Drug Info 
Phase 2
Hypertension
3 Tilarginine acetate
 Drug Info 
Phase 3
Myocardial infarction
Similar Protein(s) and Bioactivity Statistics
90% Identity
Protein ID Protein Name Protein Organism
PT01594 Nitric oxide synthase 3 Bos taurus, Bovine
 Protein Info 
PT04155 Nitric oxide synthase 3 Rattus norvegicus, Rat
 Protein Info