General Information of the Protein
Protein ID
PT01594
Protein Name
Nitric oxide synthase 3
Secondarily
Protein Name
Constitutive NOS
EC-NOS
NOS type III
Nitric oxide synthase
endothelial
Gene Name
NOS3
Sequence
MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSRAPAPATPHAPDHSPAPNSPTLTRPPEGPKFPRVKNWELGSITYDTLCAQSQQDGPCTPRCCLGSLVLPRKLQTRPSPGPPPAEQLLSQARDFINQYYSSIKRSGSQAHEERLQEVEAEVASTGTYHLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCSSAQEMFTYICNHIKYATNRGNLRSAITVFPQRAPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFSAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSFQLAKVTIVDHHAATVSFMKHLDNEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYILSPAFRYQPDPWKGSATKGAGITRKKTFKEVANAVKISASLMGTLMAKRVKATILYASETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHEALVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAKAAFQASCETFCVGEEAKAAAQDIFSPKRSWKRQRYRLSTQAEGLQLLPGLIHVHRRKMFQATVLSVENLQSSKSTRATILVRLDTAGQEGLQYQPGDHIGICPPNRPGLVEALLSRVEDPPPPTESVAVEQLEKGSPGGPPPSWVRDPRLPPCTLRQALTFFLDITSPPSPRLLRLLSTLAEEPSEQQELETLSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPNAHPGEVHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKTGDPVPCFIRGAPSFRLPPDPYVPCILVGPGTGIAPFRGFWQERLHDIESKGLQPAPMTLVFGCRCSQLDHLYRDEVQDAQERGVFGRVLTAFSREPDSPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATSVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERHLRGAVPWAFDPPGPDTPGP
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Organism
Bos taurus, Bovine
Protein Classification
Enzyme
Function
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
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Uniprot ID
P29473
Ensembl ID
ENSG00000164867
Subcellular Location
Cell membrane
Membrane
Caveola
Cytoplasm
Cytoskeleton
Golgi apparatus
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000879 , BAE1
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0067773
 Compound Info 
(2S)-2-amino-5-{[(E)-amino(nitroimino)methyl]amino}pentanoic acid
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C6H13N5O4
  1
1
IC50 = 1200 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0448799
 Compound Info 
(S)-2-Amino-5-(5-methyl-2-nitro-phenylamino)-pentanoic acid
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C12H17N3O4
  1
1
IC50 = 5000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0053263
 Compound Info 
(2S)-2-azaniumyl-5-[(N'-methylcarbamimidoyl)amino]pentanoate
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C7H16N4O2
  1
1
IC50 = 7000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0373158
 Compound Info 
(S)-2-Amino-5-(5-methyl-2-nitro-phenylsulfanyl)-pentanoic acid
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C12H16N2O4S
  1
1
IC50 = 9000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0378306
 Compound Info 
(S)-2-Amino-5-(5-methyl-2-nitro-phenoxy)-pentanoic acid
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C12H16N2O5
  1
1
IC50 = 10000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0470712
 Compound Info 
N'-(5-methyl-2-nitrophenyl)butane-1,4-diamine
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C11H17N3O2
  1
1
IC50 = 20000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0529439
 Compound Info 
(S)-2-Amino-5-(5-methyl-2-nitro-phenylamino)-pentan-1-ol
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C12H19N3O3
  1
1
IC50 = 40000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0275752
 Compound Info 
(S)-2-Amino-5-(2,6-dinitro-phenylamino)-pentanoic acid
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C11H14N4O6
  1
1
IC50 = 60000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0203577
 Compound Info 
(S)-2-Amino-5-(2-nitro-phenylamino)-pentanoic acid
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C11H15N3O4
  1
1
IC50 = 100000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0257550
 Compound Info 
(S)-2-Amino-5-(4-fluoro-2-nitro-phenylamino)-pentanoic acid
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C11H14FN3O4
  1
1
IC50 > 100000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0067773
 Compound Info 
(2S)-2-amino-5-{[(E)-amino(nitroimino)methyl]amino}pentanoic acid
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C6H13N5O4
  2
1 Ki = 720 nM
2 Ki = 750 nM
Similar Protein(s) and Bioactivity Statistics
90% Identity
Protein ID Protein Name Protein Organism
PT01856 Nitric oxide synthase 3 Homo sapiens, Human
 Protein Info 
PT04155 Nitric oxide synthase 3 Rattus norvegicus, Rat
 Protein Info