General Information of the Protein
Protein ID
PT01532
Protein Name
Mitogen-activated protein kinase kinase kinase kinase 4
Secondarily
Protein Name
HPK/GCK-like kinase HGK
MAPK/ERK kinase kinase kinase 4
Nck-interacting kinase
Gene Name
MAP4K4
Secondarily
Gene Name
HGK
KIAA0687
NIK
Sequence
MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW
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Organism
Homo sapiens, Human
Protein Classification
Enzyme
>
Kinase
>
Protein Kinase
>
STE protein kinase group
>
STE protein kinase STE20 family
>
STE protein kinase MSN subfamily
Function
Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.
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Uniprot ID
Primary ID:
O95819

Secondarily ID:
E7ESS2
O75172
Q9NST7
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Ensembl ID
ENSG00000071054
HGNC ID
HGNC:6866
Subcellular Location
Cytoplasm
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000362 , GripTite 293 MSR
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0094715
 Compound Info 
5-(6-aminopyridin-3-yl)-3-(4-chlorophenyl)pyridin-2-amine
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C16H13ClN4
  1
1
IC50 = 160 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0520574
 Compound Info 
4-[6-amino-5-(4-chlorophenyl)pyridin-3-yl]benzamide
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C18H14ClN3O
  1
1
IC50 = 360 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0094714
 Compound Info 
5-[6-amino-5-(4-chlorophenyl)pyridin-3-yl]pyrimidin-2-amine
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C15H12ClN5
  1
1
IC50 = 470 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0120822
 Compound Info 
4-[6-amino-5-(4-chlorophenyl)pyridin-3-yl]-1H-pyridin-2-one
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C16H12ClN3O
  1
1
IC50 = 590 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0201582
 Compound Info 
4-[5-(4-methoxyphenyl)pyridin-3-yl]benzamide
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C19H16N2O2
  1
1
IC50 = 600 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0329200
 Compound Info 
4-[6-amino-5-(4-methylsulfonylphenyl)pyridin-3-yl]phenol
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C18H16N2O3S
  1
1
IC50 = 2900 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0447831
 Compound Info 
5-[6-amino-5-(4-chlorophenyl)pyridin-3-yl]-1H-pyridin-2-one
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C16H12ClN3O
  1
1
IC50 = 2900 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0364385
 Compound Info 
4-[6-amino-5-(4-methylsulfonylphenyl)pyridin-3-yl]benzamide
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C19H17N3O3S
  1
1
IC50 = 4200 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0362183
 Compound Info 
4-[5-(4-cyclopropyloxyphenyl)pyridin-3-yl]benzamide
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C21H18N2O2
  1
1
IC50 = 6200 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0360388
 Compound Info 
4-[5-(4-chlorophenyl)-1,3-oxazol-2-yl]benzamide
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C16H11ClN2O2
  1
1
IC50 = 6300 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0369810
 Compound Info 
4-[5-(4-chlorophenyl)pyridin-3-yl]benzamide
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C18H13ClN2O
  1
1
IC50 = 7500 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0224038
 Compound Info 
4-[4-(4-chlorophenyl)pyrimidin-2-yl]benzamide
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C17H12ClN3O
  1
1
IC50 > 30000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0417407
 Compound Info 
4-[4-(4-chlorophenyl)pyridin-2-yl]benzamide
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C18H13ClN2O
  1
1
IC50 > 30000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0510845
 Compound Info 
4-[1-(4-chlorophenyl)imidazol-4-yl]benzamide
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C16H12ClN3O
  1
1
IC50 > 30000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0520573
 Compound Info 
4-[4-(4-chlorophenyl)pyrazol-1-yl]benzamide
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C16H12ClN3O
  1
1
IC50 > 30000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Cell Line ID: CL000012 , Sf21
 Cell Line Info 
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0094715
 Compound Info 
5-(6-aminopyridin-3-yl)-3-(4-chlorophenyl)pyridin-2-amine
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C16H13ClN4
  1
1 IC50 = 3.7 nM
CP0520574
 Compound Info 
4-[6-amino-5-(4-chlorophenyl)pyridin-3-yl]benzamide
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C18H14ClN3O
  1
1 IC50 = 13 nM
CP0094714
 Compound Info 
5-[6-amino-5-(4-chlorophenyl)pyridin-3-yl]pyrimidin-2-amine
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C15H12ClN5
  1
1 IC50 = 14.9 nM
CP0120822
 Compound Info 
4-[6-amino-5-(4-chlorophenyl)pyridin-3-yl]-1H-pyridin-2-one
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C16H12ClN3O
  1
1 IC50 = 20 nM
CP0329200
 Compound Info 
4-[6-amino-5-(4-methylsulfonylphenyl)pyridin-3-yl]phenol
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C18H16N2O3S
  1
1 IC50 = 21 nM
CP0201582
 Compound Info 
4-[5-(4-methoxyphenyl)pyridin-3-yl]benzamide
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C19H16N2O2
  1
1 IC50 = 45 nM
CP0369810
 Compound Info 
4-[5-(4-chlorophenyl)pyridin-3-yl]benzamide
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C18H13ClN2O
  1
1 IC50 = 46 nM
CP0362183
 Compound Info 
4-[5-(4-cyclopropyloxyphenyl)pyridin-3-yl]benzamide
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C21H18N2O2
  2
1 IC50 = 48 nM
2 IC50 = 9800 nM
CP0364385
 Compound Info 
4-[6-amino-5-(4-methylsulfonylphenyl)pyridin-3-yl]benzamide
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C19H17N3O3S
  1
1 IC50 = 64 nM
CP0360388
 Compound Info 
4-[5-(4-chlorophenyl)-1,3-oxazol-2-yl]benzamide
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C16H11ClN2O2
  2
1 IC50 = 94 nM
2 IC50 > 10000 nM
CP0447831
 Compound Info 
5-[6-amino-5-(4-chlorophenyl)pyridin-3-yl]-1H-pyridin-2-one
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C16H12ClN3O
  1
1 IC50 = 96 nM
CP0520573
 Compound Info 
4-[4-(4-chlorophenyl)pyrazol-1-yl]benzamide
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C16H12ClN3O
  2
1 IC50 = 248 nM
2 IC50 > 10000 nM
CP0510845
 Compound Info 
4-[1-(4-chlorophenyl)imidazol-4-yl]benzamide
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C16H12ClN3O
  1
1 IC50 = 270 nM
CP0224038
 Compound Info 
4-[4-(4-chlorophenyl)pyrimidin-2-yl]benzamide
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C17H12ClN3O
  1
1 IC50 = 861 nM
CP0417407
 Compound Info 
4-[4-(4-chlorophenyl)pyridin-2-yl]benzamide
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C18H13ClN2O
  1
1 IC50 = 1722 nM
Clinical Information about the Protein
Target 1 ( MEK kinase kinase 4 (MAP4K4) )
Target Type Patented-recorded Target