General Information of the Protein
Protein ID
PT00149
Protein Name
Nonstructural protein 5A
Gene Name
NS5A
Sequence
SGSWLRDVWDWICTVLTDFKTWLQSKLLPRIPGVPFLSCQRGYKGVWRGDGIMHTTCPCGAQITGHVKNGSMRIVGPKTCSNTWHGTFPINTYTTGPCTPSPAPNYSRALWRVAAEEYVEVTRVGDFHYVTGMTTDNVKCPCQVPAPEFFTEVDGVRLHRYAPACKPLLREEVTFMVGLNQYLVGSQLPCEPEPDVTVLTSMLTDPSHITAETAGRRLARGSPPSLASSSASQLSAPSLKATCTTRHDSPDADLIEANLLWRQEMGGNITRVESENKVVILDSFDPLQAEEDEREVSVPAEILRKSRKFPRAMPIWARPDYNPPLLESWKDPDYVPPVVHGCPLPPAKAPPIPPPRRKRTVVLTESTVSSALAELATKTFGSSGSSAVDSGTATAPPDQLPGDGDSGSDVESYSSMPPLEGEPGDPDLSDGSWSTMSEEASEDVVCC
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Organism
Hepacivirus hominis
Protein Classification
Unclassified protein
Uniprot ID
Q5L478
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000322 , Huh-7-Lunet
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0032498
 Compound Info 
CVD-0019437
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C40H50N8O6
  2
1
EC50 = 0.032 nM
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2
EC50 = 0.079 nM
 Bioactivity Info 
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CP0868535
 Compound Info 
methyl N-[(3S,6S,9aR)-3-[5-[4-[4-[2-[(2S)-1-[(2S)-2-(methoxycarbonylamino)-3-methyl-butanoyl]pyrrolidin-2-yl]-1H-imidazol-5-yl]phenyl]phenyl]-1H-imidazol-2-yl]-5-oxo-1,2,3,6,7,8,9,9a-octahydropyrrolo[1,2-a]azepin-6-yl]carbamate
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C40H48N8O6
  2
1
EC50 = 0.037 nM
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2
EC50 = 0.471 nM
 Bioactivity Info 
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CP0960971
 Compound Info 
methyl N-[(3S,6S,9aS)-3-[5-[4-[4-[2-[(8S)-7-[(2S)-2-(methoxycarbonylamino)-3-methyl-butanoyl]-1,4-dioxa-7-azaspiro[4.4]nonan-8-yl]-1H-imidazol-5-yl]phenyl]phenyl]-1H-imidazol-2-yl]-5-oxo-1,2,3,6,7,8,9,9a-octahydropyrrolo[1,2-a]azepin-6-yl]carbamate
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C42H50N8O8
  2
1
EC50 = 0.046 nM
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2
EC50 = 8.581 nM
 Bioactivity Info 
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CP0951257
 Compound Info 
methyl N-[(3S,6R,9aR)-3-[5-[4-[4-[2-[(8S)-7-[(2S)-2-(methoxycarbonylamino)-3-methyl-butanoyl]-1,4-dioxa-7-azaspiro[4.4]nonan-8-yl]-1H-imidazol-5-yl]phenyl]phenyl]-1H-imidazol-2-yl]-5-oxo-1,2,3,6,7,8,9,9a-octahydropyrrolo[1,2-a]azepin-6-yl]carbamate
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C42H50N8O8
  2
1
EC50 = 0.047 nM
 Bioactivity Info 
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2
EC50 = 0.707 nM
 Bioactivity Info 
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CP0868534
 Compound Info 
methyl N-[(3S,6R,9aR)-3-[5-[4-[4-[2-[(2S)-1-[(2S)-2-(methoxycarbonylamino)-3-methyl-butanoyl]pyrrolidin-2-yl]-1H-imidazol-5-yl]phenyl]phenyl]-1H-imidazol-2-yl]-5-oxo-1,2,3,6,7,8,9,9a-octahydropyrrolo[1,2-a]azepin-6-yl]carbamate
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C40H48N8O6
  2
1
EC50 = 0.059 nM
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2
EC50 = 0.673 nM
 Bioactivity Info 
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CP0911877
 Compound Info 
methyl N-[(3S,6S,9aR)-3-[5-[4-[4-[2-[(8S)-7-[(2S)-2-(methoxycarbonylamino)-3-methyl-butanoyl]-1,4-dioxa-7-azaspiro[4.4]nonan-8-yl]-1H-imidazol-5-yl]phenyl]phenyl]-1H-imidazol-2-yl]-5-oxo-1,2,3,6,7,8,9,9a-octahydropyrrolo[1,2-a]azepin-6-yl]carbamate
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C42H50N8O8
  2
1
EC50 = 0.064 nM
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2
EC50 = 1.524 nM
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CP0951256
 Compound Info 
methyl N-[(3S,6R,9aS)-3-[5-[4-[4-[2-[(8S)-7-[(2S)-2-(methoxycarbonylamino)-3-methyl-butanoyl]-1,4-dioxa-7-azaspiro[4.4]nonan-8-yl]-1H-imidazol-5-yl]phenyl]phenyl]-1H-imidazol-2-yl]-5-oxo-1,2,3,6,7,8,9,9a-octahydropyrrolo[1,2-a]azepin-6-yl]carbamate
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C42H50N8O8
  2
1
EC50 = 0.08 nM
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2
EC50 = 14.52 nM
 Bioactivity Info 
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CP0951258
 Compound Info 
methyl N-[(1S)-1-[(8S)-8-[5-[4-[4-[2-[(3S,6S,9aR)-6-(dimethylamino)-5-oxo-1,2,3,6,7,8,9,9a-octahydropyrrolo[1,2-a]azepin-3-yl]-1H-imidazol-5-yl]phenyl]phenyl]-1H-imidazol-2-yl]-1,4-dioxa-7-azaspiro[4.4]nonane-7-carbonyl]-2-methyl-propyl]carbamate
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C42H52N8O6
  2
1
EC50 = 0.084 nM
 Bioactivity Info 
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2
EC50 = 7.707 nM
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CP0868724
 Compound Info 
US9427428, 14::US9433609, 14
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C48H46N8O6
  1
1
EC50 = 0.093 nM
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CP0868533
 Compound Info 
methyl N-[(3S,6S,9aS)-3-[5-[4-[4-[2-[(2S)-1-[(2S)-2-(methoxycarbonylamino)-3-methyl-butanoyl]pyrrolidin-2-yl]-1H-imidazol-5-yl]phenyl]phenyl]-1H-imidazol-2-yl]-5-oxo-1,2,3,6,7,8,9,9a-octahydropyrrolo[1,2-a]azepin-6-yl]carbamate
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C40H48N8O6
  2
1
EC50 = 0.109 nM
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2
EC50 = 17.44 nM
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CP0967154
 Compound Info 
methyl N-[(3S,6R,9aS)-3-[5-[4-[4-[2-[(2S)-1-[(2S)-2-(methoxycarbonylamino)-3-methyl-butanoyl]pyrrolidin-2-yl]-1H-imidazol-5-yl]phenyl]phenyl]-1H-imidazol-2-yl]-5-oxo-1,2,3,6,7,8,9,9a-octahydropyrrolo[1,2-a]azepin-6-yl]carbamate
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C40H48N8O6
  2
1
EC50 = 0.133 nM
 Bioactivity Info 
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2
EC50 = 12.48 nM
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CP0943048
 Compound Info 
methyl N-[(1S)-1-[(8S)-8-[5-[4-[4-[2-[(3S,6S,9aS)-6-(dimethylamino)-5-oxo-1,2,3,6,7,8,9,9a-octahydropyrrolo[1,2-a]azepin-3-yl]-1H-imidazol-5-yl]phenyl]phenyl]-1H-imidazol-2-yl]-1,4-dioxa-7-azaspiro[4.4]nonane-7-carbonyl]-2-methyl-propyl]carbamate
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C42H52N8O6
  2
1
EC50 = 0.209 nM
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2
EC50 = 32.16 nM
 Bioactivity Info 
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CP0953793
 Compound Info 
methyl N-[(1S)-1-[(8S)-8-[5-[4-[4-[2-[(3S,6R,9aS)-6-(dimethylamino)-5-oxo-1,2,3,6,7,8,9,9a-octahydropyrrolo[1,2-a]azepin-3-yl]-1H-imidazol-5-yl]phenyl]phenyl]-1H-imidazol-2-yl]-1,4-dioxa-7-azaspiro[4.4]nonane-7-carbonyl]-2-methyl-propyl]carbamate
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C42H52N8O6
  2
1
EC50 = 0.217 nM
 Bioactivity Info 
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2
EC50 = 119.15 nM
 Bioactivity Info 
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CP0903192
 Compound Info 
US9427428, 12::US9433609, 12
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C46H54N8O8
  1
1
EC50 = 0.275 nM
 Bioactivity Info 
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CP0953457
 Compound Info 
methyl N-[(1S)-1-[(8S)-8-[5-[4-[4-[2-[(3S,6R,9aR)-6-(dimethylamino)-5-oxo-1,2,3,6,7,8,9,9a-octahydropyrrolo[1,2-a]azepin-3-yl]-1H-imidazol-5-yl]phenyl]phenyl]-1H-imidazol-2-yl]-1,4-dioxa-7-azaspiro[4.4]nonane-7-carbonyl]-2-methyl-propyl]carbamate
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C42H52N8O6
  2
1
EC50 = 0.298 nM
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2
EC50 = 10.63 nM
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CP0923330
 Compound Info 
US9427428, 6::US9433609, 6
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C40H46N8O8
  1
1
EC50 = 1.013 nM
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CP0969801
 Compound Info 
US9427428, 6::US9433609, 6
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C40H46N8O8
  1
1
EC50 = 1.013 nM
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CP0960189
 Compound Info 
US9427428, 10
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C42H50N8O8
  1
1
EC50 = 1.032 nM
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CP0909845
 Compound Info 
US9427428, 15
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C46H54N8O6
  1
1
EC50 = 1.192 nM
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CP0959532
 Compound Info 
US9427428, 7::US9433609, 7
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C44H54N8O6
  1
1
EC50 = 3.411 nM
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CP0926760
 Compound Info 
US9427428, 19
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C44H54N8O6
  1
1
EC50 = 5.186 nM
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CP0957624
 Compound Info 
US9433609, 19
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C44H54N8O6
  1
1
EC50 = 5.186 nM
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CP0923329
 Compound Info 
US9427428, 5::US9433609, 5
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C42H46N8O6
  1
1
EC50 = 10.85 nM
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CP0969930
 Compound Info 
US9433609, 21
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C43H52N8O7
  1
1
EC50 = 275 nM
 Bioactivity Info 
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CP0865738
 Compound Info 
US9427428, 20
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C43H52N8O7
  1
1
EC50 = 336 nM
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CP0913788
 Compound Info 
US9433609, 20
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C43H52N8O7
  1
1
EC50 = 336 nM
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Cell Line ID: CL000148 , Huh-7
 Cell Line Info 
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0029881
 Compound Info 
ABT-267
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C50H67N7O8
  3
1 EC50 = 0.014 nM
2 EC50 = 0.055 nM
3 EC50 = 0.186 nM
CP0032498
 Compound Info 
CVD-0019437
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C40H50N8O6
  5
1 EC50 = 0.05 nM
2 EC50 = 0.071 nM
3 EC50 = 0.146 nM
4 EC50 = 61 nM
5 EC50 = 1260 nM
CP0701915
 Compound Info 
Daclatasvir
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C40H51N9O6
  1
1 EC50 = 0.146 nM