General Information of the Protein
Protein ID
PT03652
Protein Name
Mitogen-activated protein kinase kinase kinase 14
Secondarily
Protein Name
NF-kappa-beta-inducing kinase
Serine/threonine-protein kinase NIK
Gene Name
MAP3K14
Secondarily
Gene Name
NIK
Sequence
MAVMEMACPGAPGSAVGQQKELPKAKEKTPPLGKKQSSVYKLEAVEKSPVFCGKWEILNDVITKGTAKEGSEAGPAAISIIAQAECENSQEFSPTFSERIFIAGSKQYSQSESLDQIPNNVAHATEGKMARVCWKGKRRSKARKKRKKKSSKSLAHAGVALAKPLPRTPEQESCTIPVQEDESPLGAPYVRNTPQFTKPLKEPGLGQLCFKQLGEGLRPALPRSELHKLISPLQCLNHVWKLHHPQDGGPLPLPTHPFPYSRLPHPFPFHPLQPWKPHPLESFLGKLACVDSQKPLPDPHLSKLACVDSPKPLPGPHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSGQAHSLTSLAKTWAARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQVGGLKSPWRGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPGPRPAEETTGRAPKLQPPLPPEPPEPNKSPPLTLSKEESGMWEPLPLSSLEPAPARNPSSPERKATVPEQELQQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSLSDDSEKNPSKASQSSRDTLSSGVHSWSSQAEARSSSWNMVLARGRPTDTPSYFNGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPAAAFSLVTKDGQPVRYDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLENRP
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Organism
Homo sapiens, Human
Protein Classification
Enzyme
>
Kinase
>
Protein Kinase
>
STE protein kinase group
>
STE protein kinase unique family
Function
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
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Uniprot ID
Primary ID:
Q99558

Secondarily ID:
A8K2D8
D3DX67
Q8IYN1
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Ensembl ID
ENSG00000006062
HGNC ID
HGNC:6853
Subcellular Location
Cytoplasm
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000006 , HEK293
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0473593
 Compound Info 
1-[3-[2-[(3R)-3-hydroxy-1- methyl-2-oxo-pyrrolidin-3- yl]ethynyl]phenyl]-6-methoxy- imidazo[1,5-a]pyridine- 3-carboxamide
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C22H20N4O4
  1
1
IC50 = 4.7 nM
 Bioactivity Info 
   TI
   LI
   LO
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CP0519772
 Compound Info 
3-[3-[2-[(3R)-3-hydroxy-1-methyl-2-oxopyrrolidin-3-yl]ethynyl]phenyl]isoquinoline-1-carboxamide
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C23H19N3O3
  1
1
IC50 = 10 nM
 Bioactivity Info 
   TI
   LI
   LO
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CP0474092
 Compound Info 
1-[3-[2-[(3R)-3-hydroxy-1- methyl-2-oxo-pyrrolidin-3- yl]ethynyl]phenyl]imidazo[1,5- a]pyridine-3-carboxamide
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C21H18N4O3
  1
1
IC50 = 15 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0415705
 Compound Info 
3-amino-6-[3-[2-[(3R)-3-hydroxy-1-methyl-2-oxopyrrolidin-3-yl]ethynyl]phenyl]pyridine-2-carboxamide
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C19H18N4O3
  1
1
IC50 = 16 nM
 Bioactivity Info 
   TI
   LI
   LO
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CP0456080
 Compound Info 
1-[3-[2-[(3R)-3-hydroxy-1- methyl-2-oxo-pyrrolidin-3- yl]ethynyl]phenyl]-5-methoxy- pyrazolo[3,4-b]pyridine- 3-carboxamide
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C21H19N5O4
  1
1
IC50 = 21 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0519770
 Compound Info 
1-[3-[2-[(3R)-3-hydroxy-1- methyl-2-oxo-pyrrolidin-3- yl]ethynyl]phenyl]indazole- 3-carboxamide
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C21H18N4O3
  1
1
IC50 = 29 nM
 Bioactivity Info 
   TI
   LI
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   TS
CP0445958
 Compound Info 
1-[4-[2-[(3R)-3-hydroxy-1- methyl-2-oxo-pyrrolidin-3- yl]ethynyl]-2-pyridyl]indazole- 3-carboxamide
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C20H17N5O3
  1
1
IC50 = 33 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0390947
 Compound Info 
6-[3-[2-[(3R)-3-hydroxy-1-methyl-2-oxopyrrolidin-3-yl]ethynyl]phenyl]-4-methoxypyridine-2-carboxamide
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C20H19N3O4
  1
1
IC50 = 34 nM
 Bioactivity Info 
   TI
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   LO
   TS
CP0456079
 Compound Info 
1-[3-[2-[(3R)-3-hydroxy-1- methyl-2-oxo-pyrrolidin-3- yl]ethynyl]phenyl]pyrazolo [3,4-b]pyridine-3-carboxamide
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C20H17N5O3
  1
1
IC50 = 38 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0450890
 Compound Info 
5-fluoro-1-[3-[2-[(3R)-3-hy- droxy-1-methyl-2-oxo-pyrroli- din-3-yl]ethynyl]phenyl] indazole-3-carboxamide
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C21H17FN4O3
  1
1
IC50 = 39 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0456083
 Compound Info 
2-[3-[2-[(3R)-3-hydroxy-1-methyl-2-oxopyrrolidin-3-yl]ethynyl]phenyl]-6-methoxypyrimidine-4-carboxamide
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C19H18N4O4
  1
1
IC50 = 69 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0519771
 Compound Info 
6-[3-[2-[(3R)-3-hydroxy-1-methyl-2-oxopyrrolidin-3-yl]ethynyl]phenyl]pyridine-2-carboxamide
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C19H17N3O3
  1
1
IC50 = 80 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0445959
 Compound Info 
1-[4-[2-[(3R)-3-hydroxy-1- methyl-2-oxo-pyrrolidin-3- yl]ethynyl]-2-pyridyl]-4,5,6,7- tetrahydroindazole-3-carbox- amide
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C20H21N5O3
  1
1
IC50 = 84 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0545207
 Compound Info 
6-[3-[2-[(3R)-3-hydroxy-1-methyl-2-oxopyrrolidin-3-yl]ethynyl]phenyl]pyrazine-2-carboxamide
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C18H16N4O3
  1
1
IC50 = 170 nM
 Bioactivity Info 
   TI
   LI
   LO
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CP0456113
 Compound Info 
5-fluoro-1-[4-[2-[(3R)-3-hy- droxy-1-methyl-2-oxo-pyrroli- din-3-yl]ethynyl]-2-pyri- diyl]indazole-3-carboxamide
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C20H16FN5O3
  1
1
IC50 = 220 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0415704
 Compound Info 
4-[3-[2-[(3R)-3-hydroxy-1-methyl-2-oxopyrrolidin-3-yl]ethynyl]phenyl]pyrimidine-2-carboxamide
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C18H16N4O3
  1
1
IC50 = 355 nM
 Bioactivity Info 
   TI
   LI
   LO
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CP0390953
 Compound Info 
7-ethoxy-3-[3-[2-[(3R)-3-hydroxy-1-methyl-2-oxopyrrolidin-3-yl]ethynyl]phenyl]imidazo[1,5-a]pyridine-1-carboxamide
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C23H22N4O4
  1
1
IC50 = 380 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0474091
 Compound Info 
3-[3-[2-[(3R)-3-hydroxy-1- methyl-2-oxo-pyrrolidin-3- yl]ethynyl]phenyl]imidazo[1,5- a]pyridine-1-carboxamide
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C21H18N4O3
  1
1
IC50 = 500 nM
 Bioactivity Info 
   TI
   LI
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   TS
CP0390956
 Compound Info 
5-cyclopropyl-1-[4-[2-[(3R)- 3-hydroxy-1-methyl-2-oxo- pyrrolidin-3-yl]ethynyl]- 2-pyridyl]pyrazole-3-carbox- amide
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C19H19N5O3
  1
1
IC50 = 2100 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0390957
 Compound Info 
5-amino-1-[4-[2-[(3R)-3-hy- droxy-1-methyl-2-oxo-pyrroli- din-3-yl]ethynyl]-2-pyri- diyl]pyrazole-3-carboxamide
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C16H16N6O3
  1
1
IC50 = 4300 nM
 Bioactivity Info 
   TI
   LI
   LO
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CP0445957
 Compound Info 
1-[4-[2-[(3R)-3-hydroxy-1- methyl-2-oxo-pyrrolidin-3- yl]ethynyl]-2-pyridyl]pyrazole- 3-carboxamide
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C16H15N5O3
  1
1
IC50 > 20000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Cell Line ID: CL000017 , HeLa
 Cell Line Info 
Cell Line ID: CL000013 , Sf9
 Cell Line Info 
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0536766
 Compound Info 
(2R)-4-[1-(2-amino-5-chloropyrimidin-4-yl)-2,3-dihydroindol-6-yl]-2-(1,3-thiazol-2-yl)but-3-yn-2-ol
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C19H16ClN5OS
  1
1 IC50 = 4.2 nM
CP0184914
 Compound Info 
4-[3-[2-amino-5-(2-methoxyethoxy)pyrimidin-4-yl]-1H-indol-5-yl]-2-methylbut-3-yn-2-ol
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C20H22N4O3
  1
1 IC50 = 40 nM
CP0130395
 Compound Info 
4-(4-amino-3,5,12-triazatetracyclo[9.7.0.02,7.013,18]octadeca-1(11),2,4,6,13(18),14,16-heptaen-16-yl)-2-methylbut-3-yn-2-ol
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C20H20N4O
  1
1 IC50 = 41 nM
CP0301711
 Compound Info 
16-bromo-3,5,12-triazatetracyclo[9.7.0.02,7.013,18]octadeca-1(11),2,4,6,13(18),14,16-heptaen-4-amine
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C15H13BrN4
  1
1 IC50 = 100 nM
CP0270758
 Compound Info 
4-(6-chloro-2-methylimidazo[1,2-a]pyridin-3-yl)pyrimidin-2-amine
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C12H10ClN5
  1
1 IC50 = 600 nM
CP0390947
 Compound Info 
6-[3-[2-[(3R)-3-hydroxy-1-methyl-2-oxopyrrolidin-3-yl]ethynyl]phenyl]-4-methoxypyridine-2-carboxamide
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C20H19N3O4
  1
1 Ki = 0.23 nM
CP0012472
 Compound Info 
US9034866, 6
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C20H17FN4O4
  1
1 Ki = 0.3 nM
CP0008664
 Compound Info 
US9034866, 129
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C22H19FN4O3
  2
1 Ki = 0.4 nM
2 Ki = 1 nM
CP0231830
 Compound Info 
4-[1-(2-amino-5-chloropyrimidin-4-yl)-2,3-dihydroindol-6-yl]-2-(1,3-thiazol-2-yl)but-3-yn-2-ol
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C19H16ClN5OS
  1
1 Ki = 0.4 nM
CP0014515
 Compound Info 
US9034866, 96
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C22H21N5O5
  1
1 Ki = 0.47 nM
Clinical Information about the Protein
Target 1 ( Serine/threonine-protein kinase NIK (MAP3K14) )
Target Type Literature-reported Target