General Information of the Protein
Protein ID
PT02717
Protein Name
Eukaryotic initiation factor 4A-I
Secondarily
Protein Name
ATP-dependent RNA helicase eIF4A-1
Gene Name
EIF4A1
Secondarily
Gene Name
DDX2A
EIF4A
Sequence
MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI
    Show/Hide
Organism
Homo sapiens, Human
Protein Classification
Enzyme
>
Hydrolase
Function
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
    Show/Hide
Uniprot ID
Primary ID:
P60842

Secondarily ID:
B2R6L8
D3DTP9
J3QLC4
P04765
Q5U018
Q61516
    Show/Hide
Ensembl ID
ENSG00000161960
HGNC ID
HGNC:3282
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000111 , MDA-MB-231
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0397395
 Compound Info 
dimethyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2,6-dicarboxylate
   Show/Hide
C29H28O9
  2
1
EC50 = 0.2 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 4 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0453495
 Compound Info 
(1R,2R,3S,3aR,8bS)-6-chloro-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-N,N-dimethyl-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxamide
   Show/Hide
C28H28ClNO6
  2
1
EC50 = 0.6 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 15 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0118102
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-6-[[(2S,3R,6R)-6-[(1R)-1,2-dihydroxyethyl]-3-methoxy-1,4-dioxan-2-yl]oxy]-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C34H38O13
  2
1
EC50 = 0.8 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 7 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0453494
 Compound Info 
(1R,2R,3S,3aR,8bS)-6-cyano-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-N,N-dimethyl-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxamide
   Show/Hide
C29H28N2O6
  2
1
EC50 = 0.8 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 35 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0462863
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-6-cyano-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C28H25NO7
  2
1
EC50 = 0.9 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 15 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0423624
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-6-acetyl-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C29H28O8
  2
1
EC50 = 1 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 30 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0462855
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-[[(2S,3R,6S)-6-(2-hydroxyethyl)-3-methoxy-1,4-dioxan-2-yl]oxy]-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C34H38O12
  2
1
EC50 = 1 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 5 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0397393
 Compound Info 
(1R,2R,3S,3aR,8bS)-3a-(4-cyanophenyl)-1,8b-dihydroxy-6,8-dimethoxy-N,N-dimethyl-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxamide
   Show/Hide
C29H28N2O6
  2
1
EC50 = 1.2 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 40 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0423642
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-3a-(4-cyanophenyl)-1,8b-dihydroxy-6,8-dimethoxy-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C28H25NO7
  2
1
EC50 = 2 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 17 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0397396
 Compound Info 
(1R,2R,3S,3aR,8bS)-6-[[(2S,3R,6R)-6-[(1S)-1,2-dihydroxyethyl]-3-methoxy-1,4-dioxan-2-yl]oxy]-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-N,N-dimethyl-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxamide
   Show/Hide
C35H41NO12
  2
1
EC50 = 3 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 19 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0423625
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-[[(2S,6S)-6-(hydroxymethyl)-1,4-dioxan-2-yl]oxy]-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C32H34O11
  2
1
EC50 = 3 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 40 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0423630
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-6-[[(2S,6R)-6-[(1R)-1,2-dihydroxyethyl]-1,4-dioxan-2-yl]oxy]-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C33H36O12
  2
1
EC50 = 3 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 14 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0423641
 Compound Info 
(1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-[[(2S,6S)-6-(hydroxymethyl)-1,4-dioxan-2-yl]oxy]-8-methoxy-3a-(4-methoxyphenyl)-N,N-dimethyl-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxamide
   Show/Hide
C33H37NO10
  2
1
EC50 = 3 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 23 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0462854
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-[[(2S,3R,6S)-6-(hydroxymethyl)-3-methoxy-1,4-dioxan-2-yl]oxy]-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C33H36O12
  2
1
EC50 = 3 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 60 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0397384
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-6-chloro-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C27H25ClO7
  2
1
EC50 = 4 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 70 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0453491
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6,8-dimethoxy-3a-(4-methylphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C28H28O7
  2
1
EC50 = 5 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 > 100 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0304472
 Compound Info 
AGLAFOLIN
   Show/Hide
C28H28O8
  2
1
EC50 = 10 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 90 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0423643
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,6,8b-trihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C27H26O8
  2
1
EC50 = 10 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 200 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0493559
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-6-(methylamino)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C28H29NO7
  2
1
EC50 = 11 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 700 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0397385
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-6-carbamoyl-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C28H27NO8
  2
1
EC50 = 29 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 450 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0397394
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-[[(2R,3R,6S)-6-(hydroxymethyl)-3-methoxy-1,4-dioxan-2-yl]oxy]-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C33H36O12
  2
1
EC50 = 35 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 240 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0423628
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-[[(2S,3S,6S)-6-(2-hydroxyethyl)-3-methoxy-1,4-dioxan-2-yl]oxy]-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C34H38O12
  2
1
EC50 = 35 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 275 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0462853
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-6-(methylcarbamoyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C29H29NO8
  2
1
EC50 = 35 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 > 2000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0397388
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-6-amino-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C27H27NO7
  2
1
EC50 = 40 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 > 2000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0453490
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-[(2S,6S)-6-(hydroxymethyl)oxan-2-yl]oxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C33H36O10
  2
1
EC50 = 50 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 2000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0397392
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-[(2S,6S)-6-(hydroxymethyl)morpholin-2-yl]oxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C32H35NO10
  2
1
EC50 = 80 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 = 3500 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0397383
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-[[(2R,3S,6S)-6-(2-hydroxyethyl)-3-methoxy-1,4-dioxan-2-yl]oxy]-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C34H38O12
  2
1
EC50 = 120 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 > 2000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0462852
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-[[(2S,3S,6S)-6-(hydroxymethyl)-3-methoxy-1,4-dioxan-2-yl]oxy]-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C33H36O12
  2
1
EC50 = 170 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 > 2500 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0423640
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-[[(2R,3R,6S)-6-(2-hydroxyethyl)-3-methoxy-1,4-dioxan-2-yl]oxy]-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C34H38O12
  2
1
EC50 > 200 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 > 3500 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0397390
 Compound Info 
(1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-8-methoxy-2-methoxycarbonyl-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-6-carboxylic acid
   Show/Hide
C28H26O9
  2
1
EC50 > 1000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 > 5000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0397381
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C27H26O7
  2
1
EC50 > 2000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 > 5000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0397382
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-[[(2R,3S,6S)-6-(hydroxymethyl)-3-methoxy-1,4-dioxan-2-yl]oxy]-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C33H36O12
  2
1
EC50 > 2000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 > 5000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0397387
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-6-(dimethylamino)-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C29H31NO7
  2
1
EC50 > 2000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
EC50 > 5000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0423636
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-(methanesulfonamido)-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C28H29NO9S
  1
1
EC50 > 5000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0423637
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-6-methyl-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C28H28O7
  1
1
EC50 > 5000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0423639
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6-[(2S,6S)-6-(hydroxymethyl)-4-methylmorpholin-2-yl]oxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C33H37NO10
  1
1
EC50 > 5000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0453523
 Compound Info 
methyl (1R,2R,3S,3aR,8bS)-6-(dimethylcarbamoyl)-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate
   Show/Hide
C30H31NO8
  1
1
EC50 > 5000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0100905
 Compound Info 
EFT-226
   Show/Hide
C28H29N3O5
  4
1 Kd = 21 nM
2 Kd = 2430 nM
3 Kd = 3190 nM
4 Kd = 9600 nM
CP0078717
 Compound Info 
(1R,2R,3S,3aR,8bS)-1,8b-dihydroxy-6,8-dimethoxy-3a-(4-methoxyphenyl)-N,N-dimethyl-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxamide
   Show/Hide
C29H31NO7
  1
1 Kd = 156 nM