Protein Information

General Information of the Protein

Protein ID	PT05156
Protein Name	Vesicular acetylcholine transporter
Secondarily Protein Name	Solute carrier family 18 member 3
Gene Name	SLC18A3
Secondarily Gene Name	Vacht Vat
Sequence	MEPTAPTGQARAAATKLSEAVGAALQEPQRQRRLVLVIVCVALLLDNMLYMVIVPIVPDYIAHMRGGSEGPTLVSEVWEPTLPPPTLANASAYLANTSASPTAAGSARSILRPRYPTESEDVKIGVLFASKAILQLLVNPLSGPFIDRMSYDVPLLIGLGVMFASTVMFAFAEDYATLFAARSLQGLGSAFADTSGIAMIADKYPEEPERSRALGVALAFISFGSLVAPPFGGILYEFAGKRVPFLVLAAVSLFDALLLLAVAKPFSAAARARANLPVGTPIHRLMLDPYIAVVAGALTTCNIPLAFLEPTIATWMKHTMAASEWEMGMVWLPAFVPHVLGVYLTVRLAARYPHLQWLYGALGLAVIGVSSCVVPACRSFAPLVVSLCGLCFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYALGPIVAGHIVHSLGFEQLSLGMGLANLLYAPVLLLLRNVGLLTRSRSERDVLLDEPPQGLYDAVRLREVQGKDGGEPCSPPGPFDGCEDDYNYYSRS Show/Hide
Organism	Rattus norvegicus, Rat
Protein Classification	Transporter > Electrochemical transporter > SLC superfamily of solute carriers > SLC18 family of vesicular amine transporters
Function	Electrogenic antiporter that exchanges one cholinergic neurotransmitter, acetylcholine or choline, with two intravesicular protons across the membrane of synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to store neurotransmitters inside the vesicles prior to their release via exocytosis (PubMed:8071310, PubMed:15485505). Determines cholinergic vesicular quantal size at presynaptic nerve terminals in developing neuro-muscular junctions with an impact on motor neuron differentiation and innervation pattern (PubMed:9182805). Part of forebrain cholinergic system, regulates hippocampal synapse transmissions that underlie spatial memory formation (By similarity). Can transport serotonin. Show/Hide
Uniprot ID	Primary ID: Q62666 Secondarily ID: Q64351 Show/Hide
Subcellular Location	Cytoplasmic vesicle Secretory vesicle Synaptic vesicle membrane

Map of Molecular Bioactivity Related to the Protein

Map of Molecular Bioactivity Related to the Protein Protein Cell Line Compound Bioactivity Value: <= 0.1 μM > 0.1 μM and <= 10 μM > 10 μM Imprecise Activity

Table of Molecular Bioactivities Related to the Protein

Cell Line ID: CL000868 , PC12

Cell Line Info

Compound ID	Compound Name	Compound Formula
CP0096000 Compound Info	(2R,3R)-3-(4-Phenyl-piperidin-1-yl)-1,2,3,4-tetrahydro-naphthalen-2-ol Show/Hide	C21H25NO	1
1	Ki = 1.32 nM Bioactivity Info	TI LI LO TS
CP0673695 Compound Info	N-{[5-(3-(1-Benzylpiperidin-4-yl)propoxy)-1-methyl-1Hindol-2-yl]methyl}-N-methylprop-2-yn-1-amine Show/Hide	C29H37N3O	1
1	Ki = 2.58 nM Bioactivity Info	TI LI LO TS
CP0373289 Compound Info	(-)-(R,R)-5-Fluoro-3-(4-phenyl-piperidin-1-yl)-1,2,3,4-tetrahydro-naphthalen-2-ol Show/Hide	C21H24FNO	1
1	Ki = 3.68 nM Bioactivity Info	TI LI LO TS
CP0414041 Compound Info	(+)-(S,S)-5-Fluoro-3-(4-phenyl-piperidin-1-yl)-1,2,3,4-tetrahydro-naphthalen-2-ol Show/Hide	C21H24FNO	1
1	Ki = 6.95 nM Bioactivity Info	TI LI LO TS
CP0456269 Compound Info	(2R,3R)-3-(4-phenyl-3,6-dihydro-2H-pyridin-1-yl)-1,2,3,4-tetrahydronaphthalen-2-ol Show/Hide	C21H23NO	1
1	Ki = 7.3 nM Bioactivity Info	TI LI LO TS
CP0526487 Compound Info	(rac)-5-Fluoro-3-(4-phenyl-piperidin-1-yl)-1,2,3,4-tetrahydro-naphthalen-2-ol Show/Hide	C21H24FNO	1
1	Ki = 10.9 nM Bioactivity Info	TI LI LO TS
CP0504563 Compound Info	1-[(2R,3R)-3-hydroxy-1,2,3,4-tetrahydronaphthalen-2-yl]-4-phenylpiperidin-4-ol Show/Hide	C21H25NO2	1
1	Ki = 11.5 nM Bioactivity Info	TI LI LO TS
CP0382941 Compound Info	(2R,3R)-5-Iodo-3-(4-phenyl-piperidin-1-yl)-1,2,3,4-tetrahydro-naphthalen-2-ol Show/Hide	C21H24INO	1
1	Ki = 15.8 nM Bioactivity Info	TI LI LO TS
CP0336312 Compound Info	(1R,6R)-6-(4-phenylpiperidin-1-yl)cyclohex-3-en-1-ol Show/Hide	C17H23NO	1
1	Ki = 18.4 nM Bioactivity Info	TI LI LO TS
CP0575295 Compound Info	(2R,3R)-3-(4-pyridin-4-ylpiperidin-1-yl)-1,2,3,4-tetrahydronaphthalen-2-ol Show/Hide	C20H24N2O	1
1	Ki = 19.3 nM Bioactivity Info	TI LI LO TS
CP0456141 Compound Info	(-)-(2R,3R)-5-(2-fluoroethoxy)-3-(4-phenylpiperidin-1-yl)-1,2,3,4-tetrahydronaphthalen-2-ol Show/Hide	C23H28FNO2	1
1	Ki = 19.6 nM Bioactivity Info	TI LI LO TS
CP0431651 Compound Info	(2R,3R)-3-(4-benzylpiperidin-1-yl)-1,2,3,4-tetrahydronaphthalen-2-ol Show/Hide	C22H27NO	1
1	Ki = 23.2 nM Bioactivity Info	TI LI LO TS
CP0088567 Compound Info	(+)-trans-(4-fluorophenyl)(1-(3-hydroxy-1,2,3,4-tetrahydronaphthalen-2-yl)piperidin-4-yl)methanone Show/Hide	C22H24FNO2	1
1	Ki = 24.4 nM Bioactivity Info	TI LI LO TS
CP0103754 Compound Info	(2R,3R)-5-Amino-3-(4-phenyl-piperidin-1-yl)-1,2,3,4-tetrahydro-naphthalen-2-ol Show/Hide	C21H26N2O	1
1	Ki = 25.1 nM Bioactivity Info	TI LI LO TS
CP0376134 Compound Info	(1R,2R,4R)-4-[(4-fluorophenyl)methoxy]-2-(4-phenylpiperidin-1-yl)cyclohexan-1-ol Show/Hide	C24H30FNO2	1
1	Ki = 25.1 nM Bioactivity Info	TI LI LO TS
CP0548304 Compound Info	(6-bromopyridin-3-yl)-[1'-[(E)-3-(4-chlorophenyl)prop-2-enyl]-5-fluorospiro[2H-indole-3,4'-piperidine]-1-yl]methanone Show/Hide	C27H24BrClFN3O	1
1	Ki = 39.2 nM Bioactivity Info	TI LI LO TS
CP0389404 Compound Info	5-Amino-3-(4-phenyl-piperidin-1-yl)-1,2,3,4-tetrahydro-naphthalen-2-ol Show/Hide	C21H26N2O	1
1	Ki = 40.6 nM Bioactivity Info	TI LI LO TS
CP0176032 Compound Info	N-methyl-N-[[1-methyl-5-(3-piperidin-1-ylpropoxy)indol-2-yl]methyl]prop-2-yn-1-amine Show/Hide	C22H31N3O	1
1	Ki = 46.3 nM Bioactivity Info	TI LI LO TS
CP0045885 Compound Info	(1R,2R)-2-(4-Phenyl-piperidin-1-yl)-cyclohexanol Show/Hide	C17H25NO	1
1	Ki = 47.4 nM Bioactivity Info	TI LI LO TS
CP0566584 Compound Info	(1R,2R,4R)-4-(3-fluoropropoxy)-2-(4-phenylpiperidin-1-yl)cyclohexan-1-ol Show/Hide	C20H30FNO2	1
1	Ki = 47.8 nM Bioactivity Info	TI LI LO TS
CP0295809 Compound Info	2-(4-Phenyl-piperidin-1-yl)-cyclohexanol Show/Hide	C17H25NO	1
1	Ki = 48.6 nM Bioactivity Info	TI LI LO TS
CP0568550 Compound Info	(2R,3R)-3-(4-phenylpiperazin-1-yl)-1,2,3,4-tetrahydronaphthalen-2-ol Show/Hide	C20H24N2O	1
1	Ki = 56 nM Bioactivity Info	TI LI LO TS
CP0527230 Compound Info	(+)-(2S,3S)-5-(2-fluoroethoxy)-3-(4-phenylpiperidin-1-yl)-1,2,3,4-tetrahydronaphthalen-2-ol Show/Hide	C23H28FNO2	1
1	Ki = 56.9 nM Bioactivity Info	TI LI LO TS
CP0415177 Compound Info	(1R,2R,5S)-5-(3-fluoropropoxy)-2-(4-phenylpiperidin-1-yl)cyclohexan-1-ol Show/Hide	C20H30FNO2	1
1	Ki = 59.6 nM Bioactivity Info	TI LI LO TS
CP0456157 Compound Info	(1R,2R,4R)-2-(4-benzylpiperidin-1-yl)-4-[(4-fluorophenyl)methoxy]cyclohexan-1-ol Show/Hide	C25H32FNO2	1
1	Ki = 110 nM Bioactivity Info	TI LI LO TS
CP0534512 Compound Info	[1'-[(E)-3-(4-chlorophenyl)prop-2-enyl]-5-fluorospiro[2H-indole-3,4'-piperidine]-1-yl]-(2-fluoropyridin-4-yl)methanone Show/Hide	C27H24ClF2N3O	1
1	Ki = 110 nM Bioactivity Info	TI LI LO TS
CP0397912 Compound Info	(2R,3R)-3-(4-piperidin-1-ylpiperidin-1-yl)-1,2,3,4-tetrahydronaphthalen-2-ol Show/Hide	C20H30N2O	1
1	Ki = 112.3 nM Bioactivity Info	TI LI LO TS
CP0465656 Compound Info	[1'-[(E)-3-(4-chlorophenyl)prop-2-enyl]-5-fluorospiro[2H-indole-3,4'-piperidine]-1-yl]-(2-chloropyridin-4-yl)methanone Show/Hide	C27H24Cl2FN3O	1
1	Ki = 153 nM Bioactivity Info	TI LI LO TS
CP0935451 Compound Info	(+/-)-(4-fluoro-N-(-6-hydroxy-7-(4-phenylpiperidin-1-yl)-5,6,7,8-tetrahydronaphthalen-1-yl)benzamide) Show/Hide	C28H29FN2O2	1
1	Ki = 156 nM Bioactivity Info	TI LI LO TS
CP0957251 Compound Info	(+/-)-(4-fluoro-N-(-7-hydroxy-6-(4-phenylpiperidin-1-yl)-5,6,7,8-tetrahydronaphthalen-1-yl)benzamide) Show/Hide	C28H29FN2O2	1
1	Ki = 188 nM Bioactivity Info	TI LI LO TS
CP0534513 Compound Info	[1'-[(E)-3-(4-chlorophenyl)prop-2-enyl]-5-fluorospiro[2H-indole-3,4'-piperidine]-1-yl]-(4-fluorophenyl)methanone Show/Hide	C28H25ClF2N2O	1
1	Ki = 243 nM Bioactivity Info	TI LI LO TS
CP0964696 Compound Info	(+/-)-1-(1-(5-(4-fluorobenzyloxy)-2-hydroxycyclohexyl)-4-phenylpiperidin-4-yl)ethanone Show/Hide	C26H32FNO3	1
1	Ki = 260 nM Bioactivity Info	TI LI LO TS
CP0876461 Compound Info	(+/-)-4-(4-fluorobenzyloxy)-2-(4-phenyl-5,6-dihydropyridin-1(2H)-yl)cyclohexanol Show/Hide	C24H28FNO2	1
1	Ki = 310 nM Bioactivity Info	TI LI LO TS
CP0415181 Compound Info	1-[(1R,2R,5R)-5-[(4-fluorophenyl)methoxy]-2-hydroxycyclohexyl]-4-phenylpiperidin-4-ol Show/Hide	C24H30FNO3	1
1	Ki = 316 nM Bioactivity Info	TI LI LO TS
CP0425237 Compound Info	2-fluoroethyl 1'-[(E)-3-(4-chlorophenyl)prop-2-enyl]-5-fluorospiro[2H-indole-3,4'-piperidine]-1-carboxylate Show/Hide	C24H25ClF2N2O2	1
1	Ki = 328 nM Bioactivity Info	TI LI LO TS
CP0568551 Compound Info	(2R,3R)-8-amino-3-(4-phenylpiperidin-1-yl)-1,2,3,4-tetrahydronaphthalen-2-ol Show/Hide	C21H26N2O	1
1	Ki = 339 nM Bioactivity Info	TI LI LO TS
CP0567169 Compound Info	[1'-[(E)-3-(4-chlorophenyl)prop-2-enyl]-5-fluorospiro[2H-indole-3,4'-piperidine]-1-yl]-(6-fluoropyridin-3-yl)methanone Show/Hide	C27H24ClF2N3O	1
1	Ki = 376 nM Bioactivity Info	TI LI LO TS
CP0008601 Compound Info	4-[4-(4-Chloro-phenyl)-4-hydroxy-piperidin-1-yl]-1-(4-fluoro-phenyl)-butan-1-one;propionate(HCl) Show/Hide	C21H23ClFNO2	1
1	Ki = 391 nM Bioactivity Info	TI LI LO TS
CP0846789 Compound Info	(+/-)-2-(4-phenyl-5,6-dihydropyridin-1(2H)-yl)cyclohexanol Show/Hide	C17H23NO	1
1	Ki = 439 nM Bioactivity Info	TI LI LO TS
CP0094482 Compound Info	1-(3,4-dimethoxyphenethyl)-4-(3-phenylpropyl)piperazine Show/Hide	C23H32N2O2	1
1	Ki = 492 nM Bioactivity Info	TI LI LO TS
CP0964697 Compound Info	(+/-)-(N-(7-(4-benzylpiperidin-1-yl)-6-hydroxy-5,6,7,8-tetrahydronaphthalen-1-yl)-4-fluorobenzamide) Show/Hide	C29H31FN2O2	1
1	Ki = 527 nM Bioactivity Info	TI LI LO TS
CP0415180 Compound Info	(1R,2R,4R)-2-(4,4-diphenylpiperidin-1-yl)-4-[(4-fluorophenyl)methoxy]cyclohexan-1-ol Show/Hide	C30H34FNO2	1
1	Ki = 603 nM Bioactivity Info	TI LI LO TS
CP0866897 Compound Info	(+/-)-2-(4-benzyl-piperidin-1-yl)-cyclohexanol Show/Hide	C18H27NO	1
1	Ki = 647 nM Bioactivity Info	TI LI LO TS
CP0870289 Compound Info	(+/-)-4-(4-fluorobenzyloxy)-2-(4-phenylpiperazin-1-yl)cyclohexanol Show/Hide	C23H29FN2O2	1
1	Ki = 697 nM Bioactivity Info	TI LI LO TS
CP0433806 Compound Info	(1R,2R)-2-(4-phenylpiperazin-1-yl)cyclohexan-1-ol Show/Hide	C16H24N2O	1
1	Ki = 838 nM Bioactivity Info	TI LI LO TS
CP0866898 Compound Info	(+/-)-1-(2-hydroxycyclohexyl)-4-phenyl-piperidin-4-ol Show/Hide	C17H25NO2	1
1	Ki = 1000 nM Bioactivity Info	TI LI LO TS
CP0919815 Compound Info	(+/-)-(1-(5-(4-fluorobenzyloxy)-2-hydroxycyclohexyl)piperidin-4-yl)-(4-fluorophenyl)methanone Show/Hide	C25H29F2NO3	1
1	Ki = 1026 nM Bioactivity Info	TI LI LO TS
CP0575287 Compound Info	(1R,2R,5S)-2-(4-benzylpiperidin-1-yl)-5-[(4-fluorophenyl)methoxy]cyclohexan-1-ol Show/Hide	C25H32FNO2	1
1	Ki = 1298 nM Bioactivity Info	TI LI LO TS
CP0575288 Compound Info	(1R,2R,4R)-4-[(4-fluorophenyl)methoxy]-2-(4-pyridin-4-ylpiperidin-1-yl)cyclohexan-1-ol Show/Hide	C23H29FN2O2	1
1	Ki = 1351 nM Bioactivity Info	TI LI LO TS
CP0957250 Compound Info	(+/-)-1-[1-(2-hydroxy-cyclohexyl)-4-phenyl-piperidin-4-yl]-ethanone Show/Hide	C19H27NO2	1
1	Ki = 1460 nM Bioactivity Info	TI LI LO TS
CP0963636 Compound Info	(+/-)-2-(ethyl(3-phenylpropyl)amino)-4-(4-fluorobenzyloxy)cyclohexanol Show/Hide	C24H32FNO2	1
1	Ki = 1605 nM Bioactivity Info	TI LI LO TS
CP0415178 Compound Info	(1R,2R,5S)-2-[ethyl(3-phenylpropyl)amino]-5-[(4-fluorophenyl)methoxy]cyclohexan-1-ol Show/Hide	C24H32FNO2	1
1	Ki = 1931 nM Bioactivity Info	TI LI LO TS
CP0555050 Compound Info	(1R,2R,5S)-5-[(4-fluorophenyl)methoxy]-2-(4-phenylpiperidin-1-yl)cyclohexan-1-ol Show/Hide	C24H30FNO2	1
1	Ki = 2387 nM Bioactivity Info	TI LI LO TS
CP0423095 Compound Info	(1R,2R,5S)-2-(4,4-diphenylpiperidin-1-yl)-5-[(4-fluorophenyl)methoxy]cyclohexan-1-ol Show/Hide	C30H34FNO2	1
1	Ki = 2733 nM Bioactivity Info	TI LI LO TS
CP0891678 Compound Info	(+/-)-5-(4-fluorobenzyloxy)-2-(4-phenyl-5,6-dihydropyridin-1(2H)-yl)cyclohexanol Show/Hide	C24H28FNO2	1
1	Ki = 2825 nM Bioactivity Info	TI LI LO TS
CP0123126 Compound Info	(4-fluorophenyl)-[1-[(1R,2R)-2-hydroxycyclohexyl]piperidin-4-yl]methanone Show/Hide	C18H24FNO2	1
1	Ki = 3239 nM Bioactivity Info	TI LI LO TS
CP0415182 Compound Info	(1R,2R)-2-(4-pyridin-4-ylpiperidin-1-yl)cyclohexan-1-ol Show/Hide	C16H24N2O	1
1	Ki = 3380 nM Bioactivity Info	TI LI LO TS
CP0071795 Compound Info	CHEBI:76010 Show/Hide	C20H19F2N3O5	1
1	Ki = 4523 nM Bioactivity Info	TI LI LO TS
CP0415179 Compound Info	(1R,2R,4R)-2-(3,4-dihydro-1H-isoquinolin-2-yl)-4-[(4-fluorophenyl)methoxy]cyclohexan-1-ol Show/Hide	C22H26FNO2	1
1	Ki = 4672 nM Bioactivity Info	TI LI LO TS
CP0914420 Compound Info	(+/-)-2-(1,4'-bipiperidin-1'-yl)-4-(4-fluorobenzyloxy)cyclohexanol Show/Hide	C23H35FN2O2	1
1	Ki = 4789 nM Bioactivity Info	TI LI LO TS
CP0568523 Compound Info	(1R,2R)-2-(4-piperidin-1-ylpiperidin-1-yl)cyclohexan-1-ol Show/Hide	C16H30N2O	1
1	Ki = 5106 nM Bioactivity Info	TI LI LO TS
CP0456270 Compound Info	(4-fluorophenyl)-[1-[(1R,2R,4S)-4-[(4-fluorophenyl)methoxy]-2-hydroxycyclohexyl]piperidin-4-yl]methanone Show/Hide	C25H29F2NO3	1
1	Ki = 5843 nM Bioactivity Info	TI LI LO TS
CP0866896 Compound Info	(+/-)-5-(4-fluorobenzyloxy)-2-(4-phenylpiperazin-1-yl)cyclohexanol Show/Hide	C23H29FN2O2	1
1	Ki = 6194 nM Bioactivity Info	TI LI LO TS
CP0891679 Compound Info	(+/-)-2-(4,4-diphenylpiperidin-1-yl)cyclohexanol Show/Hide	C23H29NO	1
1	Ki = 6708 nM Bioactivity Info	TI LI LO TS
CP0382043 Compound Info	1-[(1R,2R,4S)-4-[(4-fluorophenyl)methoxy]-2-hydroxycyclohexyl]-4-phenylpiperidin-4-ol Show/Hide	C24H30FNO3	1
1	Ki > 10000 nM Bioactivity Info	TI LI LO TS
CP0388829 Compound Info	(1R,2R,5S)-2-(3,4-dihydro-1H-isoquinolin-2-yl)-5-[(4-fluorophenyl)methoxy]cyclohexan-1-ol Show/Hide	C22H26FNO2	1
1	Ki > 10000 nM Bioactivity Info	TI LI LO TS
CP0456271 Compound Info	(1R,2R,5S)-5-[(4-fluorophenyl)methoxy]-2-(4-piperidin-1-ylpiperidin-1-yl)cyclohexan-1-ol Show/Hide	C23H35FN2O2	1
1	Ki > 10000 nM Bioactivity Info	TI LI LO TS
CP0492118 Compound Info	N-[(6R,7R)-6-(4-benzylpiperidin-1-yl)-7-hydroxy-5,6,7,8-tetrahydronaphthalen-1-yl]-4-fluorobenzamide Show/Hide	C29H31FN2O2	1
1	Ki > 10000 nM Bioactivity Info	TI LI LO TS
CP0524084 Compound Info	(1R,2R,5S)-5-[(4-fluorophenyl)methoxy]-2-(4-pyridin-4-ylpiperidin-1-yl)cyclohexan-1-ol Show/Hide	C23H29FN2O2	1
1	Ki > 10000 nM Bioactivity Info	TI LI LO TS
CP0555766 Compound Info	1-[1-[(1R,2R,4S)-4-[(4-fluorophenyl)methoxy]-2-hydroxycyclohexyl]-4-phenylpiperidin-4-yl]ethanone Show/Hide	C26H32FNO3	1
1	Ki > 10000 nM Bioactivity Info	TI LI LO TS
CP0914421 Compound Info	(+/-)-2-(3,4-dihydro-1H-isoquinolin-2-yl)-cyclohexanol Show/Hide	C15H21NO	1
1	Ki > 10000 nM Bioactivity Info	TI LI LO TS
CP0968701 Compound Info	(+/-)-2-(ethyl(3-phenylpropyl)amino)cyclohexanol Show/Hide	C17H27NO	1
1	Ki > 10000 nM Bioactivity Info	TI LI LO TS

Biochemical Assays

Compound ID	Compound Name	Compound Formula
CP0094482 Compound Info	1-(3,4-dimethoxyphenethyl)-4-(3-phenylpropyl)piperazine Show/Hide	C23H32N2O2	1
1	Ki = 50.2 nM

Cite

About MolBiC

Correspondence

Prof. Feng ZHU

Prof. Leo, Lianyi Han