General Information of the Protein
Protein ID
PT02666
Protein Name
Neuropeptide FF receptor 2
Secondarily
Protein Name
G-protein coupled receptor 74
G-protein coupled receptor HLWAR77
Neuropeptide G-protein coupled receptor
Gene Name
NPFFR2
Secondarily
Gene Name
GPR74
NPFF2
NPGPR
Sequence
MNSFFGTPAASWCLLESDVSSAPDKEAGRERRALSVQQRGGPAWSGSLEWSRQSAGDRRRLGLSRQTAKSSWSRSRDRTCCCRRAWWILVPAADRARRERFIMNEKWDTNSSENWHPIWNVNDTKHHLYSDINITYVNYYLHQPQVAAIFIISYFLIFFLCMMGNTVVCFIVMRNKHMHTVTNLFILNLAISDLLVGIFCMPITLLDNIIAGWPFGNTMCKISGLVQGISVAASVFTLVAIAVDRFQCVVYPFKPKLTIKTAFVIIMIIWVLAITIMSPSAVMLHVQEEKYYRVRLNSQNKTSPVYWCREDWPNQEMRKIYTTVLFANIYLAPLSLIVIMYGRIGISLFRAAVPHTGRKNQEQWHVVSRKKQKIIKMLLIVALLFILSWLPLWTLMMLSDYADLSPNELQIINIYIYPFAHWLAFGNSSVNPIIYGFFNENFRRGFQEAFQLQLCQKRAKPMEAYALKAKSHVLINTSNQLVQESTFQNPHGETLLYRKSAEKPQQELVMEELKETTNSSEI
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Organism
Homo sapiens, Human
Protein Classification
Membrane receptor
>
Family A G protein-coupled receptor
>
Peptide receptor (family A GPCR)
>
Short peptide receptor (family A GPCR)
>
RF amide receptor
Function
Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
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Uniprot ID
Primary ID:
Q9Y5X5

Secondarily ID:
Q96RV1
Q9NR49
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HGNC ID
HGNC:4525
Subcellular Location
Cell membrane
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000109 , COS-1
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0110442
 Compound Info 
L-Val-L-Pro-L-Asn-L-Leu-L-Pro-L-Gln-L-Arg-L-Phe-NH2
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C45H72N14O10
  1
1
EC50 = 1.7 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0084655
 Compound Info 
(R)-2-(2,2-diphenylacetamido)-5-guanidino-N-(4-hydroxybenzyl)pentanamide
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C27H31N5O3
  1
1
EC50 = 233 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0860640
 Compound Info 
1-[1-adamantylmethyleneamino]guanidine
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C12H20N4
  1
1
EC50 = 298 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0179651
 Compound Info 
Adamantane-1-carboxylic acid [(S)-1-((S)-1-carbamoyl-2-phenyl-ethylcarbamoyl)-4-guanidino-butyl]-amide
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C26H38N6O3
  2
1
EC50 = 379 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
Ki = 75 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0860641
 Compound Info 
2-(4-chloro-3-(trifluoromethyl)benzylidene)hydrazinecarboximidamide
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C9H8ClF3N4
  1
1
EC50 = 1189 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0877733
 Compound Info 
2-((5-(4-bromo-2-(trifluoromethyl)phenyl)furan-2-yl)methylene)hydrazinecarboximidamide
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C13H10BrF3N4O
  1
1
EC50 = 1661 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0874222
 Compound Info 
2-(3,4-dibromobenzylidene)hydrazinecarboximidamide
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C8H8Br2N4
  1
1
EC50 = 2195 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0398311
 Compound Info 
N-[(2S)-5-(1-aminoethylideneamino)-1-[[(2S)-1-amino-1-oxo-3-phenylpropan-2-yl]amino]-1-oxopentan-2-yl]adamantane-1-carboxamide
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C27H39N5O3
  1
1
Ki = 75 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0429184
 Compound Info 
(2S)-5-(diaminomethylideneamino)-2-[(2,2-diphenylacetyl)amino]-N-[(4-hydroxyphenyl)methyl]pentanamide
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C27H31N5O3
  1
1
Ki = 461 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Cell Line ID: CL000011 , CHO
 Cell Line Info 
Cell Line ID: CL000026 , CHO-K1
 Cell Line Info 
Cell Line ID: CL000025 , HEK-293T
 Cell Line Info 
Cell Line ID: CL000006 , HEK293
 Cell Line Info 
Cell Line ID: CL000050 , HEK293-A
 Cell Line Info 
Cell Line ID: CL000035 , NIH 3T3
 Cell Line Info 
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0089248
 Compound Info 
(2S)-2-[[(2S)-1-[(2S)-5-amino-2-[[(2S)-2-[[(2R)-2-[[(2R)-2-amino-3-(4-hydroxyphenyl)propanoyl]-methylamino]-4-methylpentanoyl]amino]-3-phenylpropanoyl]amino]-5-oxopentanoyl]pyrrolidine-2-carbonyl]amino]-N-[(2S)-1-[[(2S)-1-amino-1-oxo-3-phenylpropan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]pentanediamide
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C55H78N14O11
  1
1 EC50 = 2.7 nM
CP0045951
 Compound Info 
CAS_235433-36-0
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C54H76N14O10
  1
1 Kd = 0.21 nM
CP0229463
 Compound Info 
2-[(E)-(3,4-dibromophenyl)methylideneamino]guanidine;hydrochloride
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C8H9Br2ClN4
  1
1 Ki = 125.89 nM
Clinical Information about the Protein
Target 1 ( Neuropeptide FF receptor 2 (NPFFR2) )
Target Type Literature-reported Target