General Information of the Protein
Protein ID
PT02000
Protein Name
TRAF2 and NCK-interacting protein kinase
Gene Name
TNIK
Secondarily
Gene Name
KIAA0551
Sequence
MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRPVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVTSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPILESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPAKVKPEESRDITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPMKKVTDYSSSSEESESSEEEEEDGESETHDGTVAVSDIPRLIPTGAPGSNEQYNVGMVGTHGLETSHADSFSGSISREGTLMIRETSGEKKRSGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHSQEMDSGTEYGMGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW
    Show/Hide
Organism
Homo sapiens, Human
Protein Classification
Enzyme
>
Kinase
>
Protein Kinase
>
STE protein kinase group
>
STE protein kinase STE20 family
>
STE protein kinase MSN subfamily
Function
Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322.
    Show/Hide
Uniprot ID
Primary ID:
Q9UKE5

Secondarily ID:
A7E2A3
A8K4U1
D3DNQ6
O60298
Q8WUY7
Q9UKD8
Q9UKD9
Q9UKE0
Q9UKE1
Q9UKE2
Q9UKE3
Q9UKE4
    Show/Hide
Ensembl ID
ENSG00000154310
HGNC ID
HGNC:30765
Subcellular Location
Nucleus
Cytoplasm
Recycling endosome
Cytoplasm
Cytoskeleton
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000067 , HCT 116
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0018775
 Compound Info 
2-[3-[5-chloro-2-[3-methoxy-4-(4-methylpiperazin-1-yl)anilino]pyrimidin-4-yl]phenyl]acetonitrile
   Show/Hide
C24H25ClN6O
  1
1
IC50 = 5.4 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0004106
 Compound Info 
(2S,3R,4R,6R)-3-methoxy-2-methyl-4-(methylamino)-29-oxa-1,7,17-triazaoctacyclo[12.12.2.1^{2,6}.0^{7,28}.0^{8,13}.0^{15,19}.0^{20,27}.0^{21,26}]nonacosa-8(13),9,11,14(28),15(19),20(27),21(26),22,24-nonaen-16-one
   Show/Hide
C28H26N4O3
  1
1
IC50 = 39 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0367791
 Compound Info 
3-[2-[4-[4-(dimethylamino)piperidin-1-yl]-3-fluoroanilino]pyridin-4-yl]-4-methoxybenzonitrile
   Show/Hide
C26H28FN5O
  1
1
IC50 = 3100 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0331513
 Compound Info 
4-methoxy-3-[2-(3-methoxy-4-morpholin-4-ylanilino)pyridin-4-yl]benzonitrile
   Show/Hide
C24H24N4O3
  1
1
IC50 = 6300 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0156212
 Compound Info 
4-(5-chloro-2-methoxyphenyl)-N-[3-methoxy-4-(4-methylpiperazin-1-yl)phenyl]pyridin-2-amine
   Show/Hide
C24H27ClN4O2
  1
1
IC50 = 7900 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0129667
 Compound Info 
4-methoxy-3-[2-(4-morpholin-4-ylanilino)pyridin-4-yl]benzonitrile
   Show/Hide
C23H22N4O2
  1
1
IC50 = 26000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0019097
 Compound Info 
3-[2-[3-methoxy-4-(4-methylpiperazin-1-yl)anilino]pyridin-4-yl]benzonitrile
   Show/Hide
C24H25N5O
  1
1
IC50 = 27000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0156213
 Compound Info 
4-methoxy-3-[2-[4-(4-methylpiperazin-1-yl)anilino]pyridin-4-yl]benzonitrile
   Show/Hide
C24H25N5O
  1
1
IC50 > 30000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Cell Line ID: CL000006 , HEK293
 Cell Line Info 
Cell Line ID: CL000012 , Sf21
 Cell Line Info 
Cell Line ID: CL000013 , Sf9
 Cell Line Info 
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0004106
 Compound Info 
(2S,3R,4R,6R)-3-methoxy-2-methyl-4-(methylamino)-29-oxa-1,7,17-triazaoctacyclo[12.12.2.1^{2,6}.0^{7,28}.0^{8,13}.0^{15,19}.0^{20,27}.0^{21,26}]nonacosa-8(13),9,11,14(28),15(19),20(27),21(26),22,24-nonaen-16-one
   Show/Hide
C28H26N4O3
  5
1 IC50 = 0.314 nM
2 IC50 = 0.551 nM
3 IC50 = 1.4 nM
4 Kd = 4.7 nM
5 Kd = 6 nM
CP0156212
 Compound Info 
4-(5-chloro-2-methoxyphenyl)-N-[3-methoxy-4-(4-methylpiperazin-1-yl)phenyl]pyridin-2-amine
   Show/Hide
C24H27ClN4O2
  1
1 IC50 = 4 nM
CP0156213
 Compound Info 
4-methoxy-3-[2-[4-(4-methylpiperazin-1-yl)anilino]pyridin-4-yl]benzonitrile
   Show/Hide
C24H25N5O
  1
1 IC50 = 5 nM
CP0019097
 Compound Info 
3-[2-[3-methoxy-4-(4-methylpiperazin-1-yl)anilino]pyridin-4-yl]benzonitrile
   Show/Hide
C24H25N5O
  1
1 IC50 = 6 nM
CP0331513
 Compound Info 
4-methoxy-3-[2-(3-methoxy-4-morpholin-4-ylanilino)pyridin-4-yl]benzonitrile
   Show/Hide
C24H24N4O3
  1
1 IC50 = 8 nM
CP0129667
 Compound Info 
4-methoxy-3-[2-(4-morpholin-4-ylanilino)pyridin-4-yl]benzonitrile
   Show/Hide
C23H22N4O2
  1
1 IC50 = 11 nM
CP0367791
 Compound Info 
3-[2-[4-[4-(dimethylamino)piperidin-1-yl]-3-fluoroanilino]pyridin-4-yl]-4-methoxybenzonitrile
   Show/Hide
C26H28FN5O
  1
1 IC50 = 11 nM
CP0018775
 Compound Info 
2-[3-[5-chloro-2-[3-methoxy-4-(4-methylpiperazin-1-yl)anilino]pyrimidin-4-yl]phenyl]acetonitrile
   Show/Hide
C24H25ClN6O
  2
1 IC50 = 65 nM
2 Kd = 86 nM
CP0534310
 Compound Info 
2-anilino-5-[(4-methylbenzoyl)amino]-1,3-thiazole-4-carboxamide
   Show/Hide
C18H16N4O2S
  1
1 Ki = 100 nM
Clinical Information about the Protein
Target 1 ( TRAF2 and NCK interacting kinase (TNIK) )
Target Type Literature-reported Target