General Information of the Protein
Protein ID
PT01820
Protein Name
Aminopeptidase N
Secondarily
Protein Name
Alanyl aminopeptidase
Aminopeptidase M
Microsomal aminopeptidase
Myeloid plasma membrane glycoprotein CD13
gp150
Gene Name
ANPEP
Secondarily
Gene Name
APN
CD13
PEPN
Sequence
MAKGFYISKSLGILGILLGVAAVCTIIALSVVYSQEKNKNANSSPVASTTPSASATTNPASATTLDQSKAWNRYRLPNTLKPDSYRVTLRPYLTPNDRGLYVFKGSSTVRFTCKEATDVIIIHSKKLNYTLSQGHRVVLRGVGGSQPPDIDKTELVEPTEYLVVHLKGSLVKDSQYEMDSEFEGELADDLAGFYRSEYMEGNVRKVVATTQMQAADARKSFPCFDEPAMKAEFNITLIHPKDLTALSNMLPKGPSTPLPEDPNWNVTEFHTTPKMSTYLLAFIVSEFDYVEKQASNGVLIRIWARPSAIAAGHGDYALNVTGPILNFFAGHYDTPYPLPKSDQIGLPDFNAGAMENWGLVTYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNLVTIEWWNDLWLNEGFASYVEYLGADYAEPTWNLKDLMVLNDVYRVMAVDALASSHPLSTPASEINTPAQISELFDAISYSKGASVLRMLSSFLSEDVFKQGLASYLHTFAYQNTIYLNLWDHLQEAVNNRSIQLPTTVRDIMNRWTLQMGFPVITVDTSTGTLSQEHFLLDPDSNVTRPSEFNYVWIVPITSIRDGRQQQDYWLIDVRAQNDLFSTSGNEWVLLNLNVTGYYRVNYDEENWRKIQTQLQRDHSAIPVINRAQIINDAFNLASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKNYLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWMENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWILNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQWFTENSK
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Organism
Homo sapiens, Human
Protein Classification
Enzyme
>
Protease
>
Metallo protease
>
Metallo protease MAE clan
>
Metallo protease M1 family
Function
Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (By similarity).
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Uniprot ID
Primary ID:
P15144

Secondarily ID:
Q16728
Q6GT90
Q8IUK3
Q8IVH3
Q9UCE0
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Ensembl ID
ENSG00000166825
HGNC ID
HGNC:500
Subcellular Location
Cell membrane
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000377 , ES-2
Compound ID Compound Name Compound Formula
CP0472906
(2S)-2-[(2-bromophenyl)carbamoylamino]-N-hydroxy-4-methylpentanamide
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C13H18BrN3O3
 1
1
IC50 = 530 nM
   TI
   LI
   LO
   TS
CP0391667
(2S)-N-hydroxy-4-methyl-2-[(2-methylphenyl)carbamoylamino]pentanamide
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C14H21N3O3
 1
1
IC50 = 610 nM
   TI
   LI
   LO
   TS
CP0460222
(2S)-2-[(2-chlorophenyl)methylcarbamoylamino]-N-hydroxy-4-methylpentanamide
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C14H20ClN3O3
 1
1
IC50 = 650 nM
   TI
   LI
   LO
   TS
CP0434043
(2S)-N-hydroxy-2-[(2-methoxyphenyl)carbamoylamino]-4-methylpentanamide
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C14H21N3O4
 1
1
IC50 = 700 nM
   TI
   LI
   LO
   TS
CP0565803
(2S)-2-[(2-bromophenyl)methylcarbamoylamino]-N-hydroxy-4-methylpentanamide
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C14H20BrN3O3
 1
1
IC50 = 730 nM
   TI
   LI
   LO
   TS
CP0393151
(2S)-N-hydroxy-4-methyl-2-[[1-[(2-phenylmethoxyphenyl)methyl]triazol-4-yl]methylcarbamoylamino]pentanamide
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C24H30N6O4
 1
1
IC50 = 740 nM
   TI
   LI
   LO
   TS
CP0424085
(2S)-N-hydroxy-4-methyl-2-[[1-[(3-phenylmethoxyphenyl)methyl]triazol-4-yl]methylcarbamoylamino]pentanamide
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C24H30N6O4
 1
1
IC50 = 870 nM
   TI
   LI
   LO
   TS
CP0515615
(2S)-2-[(2-chlorophenyl)carbamoylamino]-N-hydroxy-4-methylpentanamide
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C13H18ClN3O3
 1
1
IC50 = 1310 nM
   TI
   LI
   LO
   TS
CP0566687
(2S)-2-[[1-[(2-ethoxyphenyl)methyl]triazol-4-yl]methylcarbamoylamino]-N-hydroxy-4-methylpentanamide
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C19H28N6O4
 1
1
IC50 = 2100 nM
   TI
   LI
   LO
   TS
CP0508627
2-(5'-chloro-2'-oxospiro[1,3-dioxepane-2,3'-indole]-1'-yl)-N-hydroxyacetamide
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C14H15ClN2O5
 1
1
IC50 = 3120 nM
   TI
   LI
   LO
   TS
CP0424086
(2S)-N-hydroxy-2-[[1-[1-(2-methoxyphenyl)ethyl]triazol-4-yl]methylcarbamoylamino]-4-methylpentanamide
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C19H28N6O4
 1
1
IC50 = 4800 nM
   TI
   LI
   LO
   TS
CP0560419
(2S)-N-hydroxy-2-[[1-[(2-methoxyphenyl)methyl]triazol-4-yl]methylcarbamoylamino]-4-methylpentanamide
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C18H26N6O4
 1
1
IC50 = 6500 nM
   TI
   LI
   LO
   TS
CP0472907
(2S)-N-hydroxy-4-methyl-2-[[1-[(2-methylphenyl)methyl]triazol-4-yl]methylcarbamoylamino]pentanamide
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C18H26N6O3
 1
1
IC50 = 6900 nM
   TI
   LI
   LO
   TS
CP0465631
2-(5'-chloro-2'-oxospiro[1,3-dioxolane-2,3'-indole]-1'-yl)-N-hydroxyacetamide
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C12H11ClN2O5
 1
1
IC50 = 9580 nM
   TI
   LI
   LO
   TS
CP0539480
2-(5'-chloro-2'-oxospiro[1,3-dioxane-2,3'-indole]-1'-yl)-N-hydroxyacetamide
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C13H13ClN2O5
 1
1
IC50 = 10940 nM
   TI
   LI
   LO
   TS
CP0444009
2-(5'-bromo-2'-oxospiro[1,3-dioxepane-2,3'-indole]-1'-yl)-N-hydroxyacetamide
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C14H15BrN2O5
 1
1
IC50 = 12100 nM
   TI
   LI
   LO
   TS
CP0413707
2-(5'-bromo-2'-oxospiro[1,3-dioxolane-2,3'-indole]-1'-yl)-N-hydroxyacetamide
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C12H11BrN2O5
 1
1
IC50 = 12400 nM
   TI
   LI
   LO
   TS
CP0539346
2-(5'-fluoro-2'-oxospiro[1,3-dioxepane-2,3'-indole]-1'-yl)-N-hydroxyacetamide
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C14H15FN2O5
 1
1
IC50 = 15160 nM
   TI
   LI
   LO
   TS
CP0558459
2-(5'-fluoro-2'-oxospiro[1,3-dioxolane-2,3'-indole]-1'-yl)-N-hydroxyacetamide
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C12H11FN2O5
 1
1
IC50 = 16190 nM
   TI
   LI
   LO
   TS
CP0539348
2-(5'-bromo-2'-oxospiro[1,3-dioxane-2,3'-indole]-1'-yl)-N-hydroxyacetamide
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C13H13BrN2O5
 1
1
IC50 = 19660 nM
   TI
   LI
   LO
   TS
CP0037780
(2S)-2-[(2S,3R)-3-amino-2-hydroxy-4-phenylbutanamido]-4-methylpentanoic acid
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C16H24N2O4
 3
1
IC50 = 20120 nM
   TI
   LI
   LO
   TS
2
IC50 = 29670 nM
   TI
   LI
   LO
   TS
3
IC50 = 35500 nM
   TI
   LI
   LO
   TS
CP0527484
2-(5'-fluoro-2'-oxospiro[1,3-dioxane-2,3'-indole]-1'-yl)-N-hydroxyacetamide
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C13H13FN2O5
 1
1
IC50 = 97490 nM
   TI
   LI
   LO
   TS
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0037780
(2S)-2-[(2S,3R)-3-amino-2-hydroxy-4-phenylbutanamido]-4-methylpentanoic acid
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C16H24N2O4
 12
1 IC50 = 800 nM
2 IC50 = 2400 nM
3 IC50 = 2500 nM
4 IC50 = 2626.629483 nM
5 IC50 = 2700 nM
6 IC50 = 3500 nM
7 IC50 = 3900 nM
8 IC50 = 16000 nM
9 IC50 = 19400 nM
10 IC50 = 35500 nM
11 IC50 = 40000 nM
12 IC50 = 54700 nM
Clinical Information about the Protein
Target 1 ( Aminopeptidase N (ANPEP) )
Target Type Clinical trial Target