General Information of the Protein
Protein ID
PT01736
Protein Name
Muscarinic acetylcholine receptor M4
Gene Name
CHRM4
Secondarily
Gene Name
Chrm-4
Sequence
MXNFTPVNGSSANQSVRLVTAAHNHLETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLGCADLIIGAFSMNLYTLYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKPSIKKPPPGGASREELRNGKLEEAPPPALPPPPRPVPDKDTSNESSSGSATQNTKERPPTELSTAEATTPALPAPTLQPRTLNPASKWSKIQIVTKQTGNECVTAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPERVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRNIGTAR
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Organism
Rattus norvegicus, Rat
Protein Classification
Membrane receptor
>
Family A G protein-coupled receptor
>
Small molecule receptor (family A GPCR)
>
Monoamine receptor
>
Acetylcholine receptor
Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
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Uniprot ID
P08485
Ensembl ID
ENSG00000180720
Subcellular Location
Cell membrane
Postsynaptic cell membrane
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000047 , 108CC15
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0673968
 Compound Info 
SID50106181
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C21H26INO2
  1
1
Ki = 0.5129 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0055122
 Compound Info 
CAS_80880-90-6
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C19H22N4O2S
  1
1
Ki = 17.4 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0384751
 Compound Info 
(6-{4-[2-Oxo-2-(6-oxo-5,6-dihydro-benzo[e]pyrido[3,2-b][1,4]diazepin-11-yl)-ethyl]-piperazin-1-yl}-hexyl)-carbamic acid tert-butyl ester
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C29H40N6O4
  1
1
Ki = 23.2 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0010104
 Compound Info 
N,N'-bis[6-[(2-methoxyphenyl)methylamino]hexyl]octane-1,8-diamine
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C36H62N4O2
  1
1
Ki = 26.3 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0562325
 Compound Info 
[1-[6-[8-[6-[4-(2-hydroxy-2,2-diphenylacetyl)oxypiperidin-1-yl]hexyl-methylamino]octyl-methylamino]hexyl]piperidin-4-yl] 2-hydroxy-2,2-diphenylacetate
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C60H86N4O6
  1
1
Ki = 31.62 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0056034
 Compound Info 
11-[(4-methylpiperazin-1-yl)acetyl]-5,11-dihydro-6H-pyrido[2,3-b][1,4]benzodiazepin-6-one
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C19H21N5O2
  2
1
Ki = 34.67 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
Ki = 330 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0432639
 Compound Info 
[1-[6-[8-[6-[4-(2,2-diphenylacetyl)oxypiperidin-1-yl]hexyl-methylamino]octyl-methylamino]hexyl]piperidin-4-yl] 2,2-diphenylacetate
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C60H86N4O4
  1
1
Ki = 39.81 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0406343
 Compound Info 
11-{2-[4-(2-Chloro-ethyl)-piperazin-1-yl]-acetyl}-5,11-dihydro-benzo[e]pyrido[3,2-b][1,4]diazepin-6-one
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C20H22ClN5O2
  1
1
Ki = 130 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0508633
 Compound Info 
2-(4-{4-[2-Oxo-2-(6-oxo-5,6-dihydro-benzo[e]pyrido[3,2-b][1,4]diazepin-11-yl)-ethyl]-piperazin-1-yl}-butyl)-isoindole-1,3-dione
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C30H30N6O4
  1
1
Ki = 210 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0440213
 Compound Info 
11-[2-(4-Benzyl-piperazin-1-yl)-acetyl]-5,11-dihydro-benzo[e]pyrido[3,2-b][1,4]diazepin-6-one
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C25H25N5O2
  1
1
Ki = 220 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0466538
 Compound Info 
Spirotramine
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C60H82N4O6
  1
1
Ki = 537.03 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0402503
 Compound Info 
N-(2-aminoethyl)-4-[[4-[2-oxo-2-(6-oxo-5H-pyrido[2,3-b][1,4]benzodiazepin-11-yl)ethyl]piperazin-1-yl]methyl]benzamide
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C28H31N7O3
  1
1
Ki = 1040 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0406182
 Compound Info 
2-(2-piperazin-1-ylacetyl)-2,4,9,15-tetrazatricyclo[9.4.0.03,8]pentadeca-1(11),3(8),4,6,12,14-hexaen-10-one
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C17H18N6O2
  1
1
Ki = 1670 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0402515
 Compound Info 
N-[2-[4-[2-oxo-2-(6-oxo-5H-pyrido[2,3-b][1,4]benzodiazepin-11-yl)ethyl]piperazin-1-yl]ethyl]acetamide
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C22H26N6O3
  1
1
Ki = 2570 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0457434
 Compound Info 
11-{2-[4-(2-Amino-ethyl)-piperazin-1-yl]-acetyl}-5,11-dihydro-benzo[e]pyrido[3,2-b][1,4]diazepin-6-one
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C20H24N6O2
  1
1
Ki = 2840 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0403763
 Compound Info 
N-methyl-4-[[4-[2-oxo-2-(6-oxo-5H-pyrido[2,3-b][1,4]benzodiazepin-11-yl)ethyl]piperazin-1-yl]methyl]benzamide
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C27H28N6O3
  1
1
Ki = 2940 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0427351
 Compound Info 
4-[2-Oxo-2-(6-oxo-5,6-dihydro-benzo[e]pyrido[3,2-b][1,4]diazepin-11-yl)-ethyl]-piperazine-1-carbothioic acid phenylamide
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C25H24N6O2S
  1
1
Ki > 100000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0436010
 Compound Info 
11-[2-(4-Acetyl-piperazin-1-yl)-acetyl]-5,11-dihydro-benzo[e]pyrido[3,2-b][1,4]diazepin-6-one
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C20H21N5O3
  1
1
Ki > 100000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0490155
 Compound Info 
11-[2-(4-Benzoyl-piperazin-1-yl)-acetyl]-5,11-dihydro-benzo[e]pyrido[3,2-b][1,4]diazepin-6-one
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C25H23N5O3
  1
1
Ki > 100000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0497274
 Compound Info 
11-[2-(4-Methanesulfonyl-piperazin-1-yl)-acetyl]-5,11-dihydro-benzo[e]pyrido[3,2-b][1,4]diazepin-6-one
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C19H21N5O4S
  1
1
Ki > 100000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Cell Line ID: CL000011 , CHO
 Cell Line Info 
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0180462
 Compound Info 
3-amino-4,6-dimethyl-N-p-anisyl-thieno[2,3-b]pyridine-2-carboxamide
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C18H19N3O2S
  1
1 EC50 = 230 nM
CP0058613
 Compound Info 
CAS_132947
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C64H77N13O6
  1
1 Ki = 11.48 nM
CP0010104
 Compound Info 
N,N'-bis[6-[(2-methoxyphenyl)methylamino]hexyl]octane-1,8-diamine
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C36H62N4O2
  1
1 Ki = 32.36 nM
Similar Protein(s) and Bioactivity Statistics
90% Identity
Protein ID Protein Name Protein Organism
PT01156 Muscarinic acetylcholine receptor M4 Homo sapiens, Human
 Protein Info