General Information of the Protein
Protein ID
PT01675
Protein Name
Wee1-like protein kinase
Secondarily
Protein Name
Wee1A kinase
Gene Name
WEE1
Sequence
MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY
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Organism
Homo sapiens, Human
Protein Classification
Enzyme
>
Kinase
>
Protein Kinase
>
Other protein kinase group
>
Other protein kinase WEE family
Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
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Uniprot ID
Primary ID:
P30291

Secondarily ID:
B3KVE1
D3DQV0
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Ensembl ID
ENSG00000166483
HGNC ID
HGNC:12761
Subcellular Location
Nucleus
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000608 , A-427
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0311473
 Compound Info 
US11332473, Compound 1.45
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C28H30N8O
  1
1
IC50 = 66 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0244995
 Compound Info 
US11332473, Compound 1.38
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C25H24N8O
  1
1
IC50 = 176 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0244996
 Compound Info 
US11332473, Compound 1.249
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C25H28FN7O2
  1
1
IC50 = 230 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0311471
 Compound Info 
US11332473, Compound 1.43
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C26H26N8O
  1
1
IC50 = 320 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0492516
 Compound Info 
US11332473, Compound 1.1
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C25H27F2N7O
  1
1
IC50 = 339 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0492515
 Compound Info 
US11332473, Compound 1.4
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C25H27F2N7O
  1
1
IC50 = 414 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0362160
 Compound Info 
US11332473, Compound 1.2
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C25H25F2N7O
  1
1
IC50 = 952 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Cell Line ID: CL000496 , AsPC-1
 Cell Line Info 
Cell Line ID: CL000095 , NCI-H1299
 Cell Line Info 
Cell Line ID: CL000013 , Sf9
 Cell Line Info 
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0096174
 Compound Info 
AZD-1775
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C27H32N8O2
  5
1 IC50 = 3.9 nM
2 IC50 = 5.2 nM
3 IC50 = 18.7 nM
4 IC50 = 22 nM
5 IC50 = 101 nM
CP0114545
 Compound Info 
7-(2,6-dichlorophenyl)-12-[(2-methylspiro[1,3-dihydroisoquinoline-4,1'-cyclopropane]-7-yl)amino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C26H21Cl2N7O
  1
1 Ki < 1 nM
CP0114546
 Compound Info 
7-(2,6-dichlorophenyl)-12-[(2,4,4-trimethyl-1,3-dihydroisoquinolin-7-yl)amino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C26H23Cl2N7O
  1
1 Ki < 1 nM
CP0142791
 Compound Info 
7-(2-chloro-6-fluorophenyl)-12-[(2-methylspiro[1,3-dihydroisoquinoline-4,1'-cyclopropane]-7-yl)amino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C26H21ClFN7O
  1
1 Ki < 1 nM
CP0360777
 Compound Info 
7-(2-chlorophenyl)-12-[(2,4,4-trimethyl-1,3-dihydroisoquinolin-7-yl)amino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C26H24ClN7O
  1
1 Ki < 1 nM
CP0379390
 Compound Info 
7-(2,6-dichlorophenyl)-12-[4-(4-methylpiperazin-1-yl)anilino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C25H22Cl2N8O
  1
1 Ki < 1 nM
CP0425982
 Compound Info 
7-(2-chloro-6-fluorophenyl)-12-[(2,4,4-trimethyl-1,3-dihydroisoquinolin-7-yl)amino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C26H23ClFN7O
  1
1 Ki < 1 nM
CP0142744
 Compound Info 
7-(2-chlorophenyl)-12-[(2-methylspiro[1,3-dihydroisoquinoline-4,1'-cyclopropane]-7-yl)amino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C26H22ClN7O
  1
1 Ki = 1.2 nM
CP0053076
 Compound Info 
7-(2-chlorophenyl)-12-[(2-methyl-3,4-dihydro-1H-isoquinolin-7-yl)amino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C24H20ClN7O
  1
1 Ki = 1.6 nM
CP0410611
 Compound Info 
7-(2-chloro-6-fluorophenyl)-12-[4-(4-methylpiperazin-1-yl)anilino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C25H22ClFN8O
  1
1 Ki = 2 nM
CP0536595
 Compound Info 
7-(2-chlorophenyl)-12-[4-(4-methylpiperazin-1-yl)anilino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C25H23ClN8O
  1
1 Ki = 2.3 nM
CP0114544
 Compound Info 
12-[(2-acetylspiro[1,3-dihydroisoquinoline-4,1'-cyclopropane]-7-yl)amino]-7-(2-chlorophenyl)-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C27H22ClN7O2
  1
1 Ki = 5.4 nM
CP0360776
 Compound Info 
7-(2-chlorophenyl)-12-(4-piperidin-1-ylanilino)-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C25H22ClN7O
  1
1 Ki = 5.5 nM
CP0271792
 Compound Info 
7-(2-chlorophenyl)-12-[3-(4-methylpiperazin-1-yl)anilino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C25H23ClN8O
  1
1 Ki = 6.2 nM
CP0142741
 Compound Info 
12-[4-(4-acetylpiperazin-1-yl)anilino]-7-(2-chlorophenyl)-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C26H23ClN8O2
  1
1 Ki = 6.6 nM
CP0142743
 Compound Info 
7-(2-chlorophenyl)-12-[(1-methyl-3,4-dihydro-2H-quinolin-6-yl)amino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C24H20ClN7O
  1
1 Ki = 7.4 nM
CP0550921
 Compound Info 
7-(2-chlorophenyl)-12-[4-(1-methylpiperidin-4-yl)anilino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C26H24ClN7O
  1
1 Ki = 8.3 nM
CP0142742
 Compound Info 
7-(2-chlorophenyl)-12-[(1-methyl-3,4-dihydro-2H-quinolin-7-yl)amino]-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C24H20ClN7O
  1
1 Ki = 8.6 nM
CP0120020
 Compound Info 
12-anilino-7-(2-chlorophenyl)-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C20H13ClN6O
  1
1 Ki = 21 nM
CP0554558
 Compound Info 
7-(2-chlorophenyl)-12-(5,6,7,8-tetrahydronaphthalen-2-ylamino)-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C24H19ClN6O
  1
1 Ki = 26 nM
CP0142740
 Compound Info 
7-(2-chlorophenyl)-12-(4-cyclohexylanilino)-2,5,7,11,13-pentazatricyclo[7.4.0.02,6]trideca-1(13),3,5,9,11-pentaen-8-one
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C26H23ClN6O
  1
1 Ki = 87 nM
Clinical Information about the Protein
Target 1 ( Wee1-like protein kinase (WEE1) )
Target Type Clinical trial Target
Disease 1 Target-related Disease  1
1 Ovarian cancer [ICD-11: 2C73]
Clinical Trial Drug(s) 1 Clinical Trial Drug  1
1 MK-1775
 Drug Info 
Phase 2
Ovarian cancer
Target 2 ( WEE1 messenger RNA (WEE1 mRNA) )
Target Type Literature-reported Target