General Information of the Protein
Protein ID
PT01106
Protein Name
Bifunctional epoxide hydrolase 2
Secondarily
Protein Name
Epoxide hydratase
Soluble epoxide hydrolase
Gene Name
EPHX2
Sequence
MALRVAAFDLDGVLALPSIAGVLRHTEEALALPRDFLLGAFQMKFPEGPTEQLMKGKITFSQWVPLMDESCRKSSKACGASLPENFSISEIFSQAMAARSINRPMLQAAAALKKKGFTTCIVTNNWLDDSDKRDILAQMMCELSQHFDFLIESCQVGMIKPEPQIYKFVLDTLKAKPNEVVFLDDFGSNLKPARDMGMVTILVRDTASALRELEKVTGTQFPEAPLPVPCSPNDVSHGYVTVKPGIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPNPEVSPMEVIRSIPVFNYQLYFQEPGVAEAELEKNMSRTFKSFFRTSDDMGLLTVNKATEMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALGRKILVPALMVTAEKDIVLRPEMSKNMENWIPFLKRGHIEDCGHWTQIEKPAEVNQILIKWLKTEIQNPSVTSKI
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Organism
Rattus norvegicus, Rat
Protein Classification
Enzyme
Function
Bifunctional enzyme (PubMed:8626766, PubMed:12574508). The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides (PubMed:8626766). Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides (PubMed:8626766). Also determines steady-state levels of physiological mediators (By similarity). The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid (By similarity).
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Uniprot ID
P80299
Ensembl ID
ENSG00000120915
Subcellular Location
Cytoplasm
Peroxisome
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000013 , Sf9
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0443467
 Compound Info 
9-(2,6-difluorobenzoyl)-N-[4-(trifluoromethoxy)phenyl]-2,9-diazaspiro[5.5]undecane-2-carboxamide
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C24H24F5N3O3
  1
1
IC50 = 3.3 nM
 Bioactivity Info 
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CP0175259
 Compound Info 
8-(2-fluoro-5-hydroxybenzoyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C23H23F4N3O4
  1
1
IC50 = 4.1 nM
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CP0149641
 Compound Info 
8-(2,6-difluorobenzoyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C23H22F5N3O3
  1
1
IC50 = 4.5 nM
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CP0339347
 Compound Info 
N-[4-(trifluoromethoxy)phenyl]-8-[1-(trifluoromethyl)cyclopropanecarbonyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C21H23F6N3O3
  1
1
IC50 = 4.7 nM
 Bioactivity Info 
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CP0175589
 Compound Info 
8-(2-chlorobenzoyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C23H23ClF3N3O3
  1
1
IC50 = 5.2 nM
 Bioactivity Info 
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CP0147504
 Compound Info 
8-(5-methyl-1,2-oxazole-3-carbonyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C21H23F3N4O4
  1
1
IC50 = 5.3 nM
 Bioactivity Info 
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CP0147507
 Compound Info 
8-(2-fluorobenzoyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C23H23F4N3O3
  1
1
IC50 = 5.3 nM
 Bioactivity Info 
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CP0149634
 Compound Info 
8-(2,6-difluorobenzoyl)-N-[4-(trifluoromethyl)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C23H22F5N3O2
  1
1
IC50 = 6.1 nM
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CP0339320
 Compound Info 
8-(2-cyclopropylacetyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C21H26F3N3O3
  1
1
IC50 = 6.4 nM
 Bioactivity Info 
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CP0175585
 Compound Info 
8-(quinoline-5-carbonyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C26H25F3N4O3
  1
1
IC50 = 6.6 nM
 Bioactivity Info 
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CP0434199
 Compound Info 
9-(2-acetamidobenzoyl)-N-[4-(trifluoromethyl)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C25H27F3N4O4
  1
1
IC50 = 6.8 nM
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CP0430056
 Compound Info 
9-(5-methylpyrazine-2-carbonyl)-N-[4-(trifluoromethoxy)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C22H24F3N5O4
  1
1
IC50 = 8.8 nM
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CP0339348
 Compound Info 
8-(cyclohexanecarbonyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C23H30F3N3O3
  1
1
IC50 = 9 nM
 Bioactivity Info 
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CP0431714
 Compound Info 
9-(2-fluorobenzoyl)-N-[4-(trifluoromethoxy)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C23H23F4N3O4
  1
1
IC50 = 9.3 nM
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CP0339289
 Compound Info 
8-(2,2-dimethylpropanoyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C21H28F3N3O3
  1
1
IC50 = 9.4 nM
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CP0147503
 Compound Info 
8-(furan-2-carbonyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C21H22F3N3O4
  1
1
IC50 = 9.5 nM
 Bioactivity Info 
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CP0439867
 Compound Info 
9-(4-chloropyridine-2-carbonyl)-N-[4-(trifluoromethoxy)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C22H22ClF3N4O4
  1
1
IC50 = 11.6 nM
 Bioactivity Info 
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CP0339322
 Compound Info 
8-(5-cyclopropyl-1,2-oxazole-4-carbonyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C23H25F3N4O4
  1
1
IC50 = 11.7 nM
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CP0339293
 Compound Info 
8-(5-methylpyrazine-2-carbonyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C22H24F3N5O3
  1
1
IC50 = 11.9 nM
 Bioactivity Info 
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CP0421093
 Compound Info 
9-(2,2-dimethylpropanoyl)-N-[4-(trifluoromethyl)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C21H28F3N3O3
  1
1
IC50 = 12 nM
 Bioactivity Info 
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CP0074784
 Compound Info 
8-(2-methylcyclopropanecarbonyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C21H26F3N3O3
  1
1
IC50 = 12.9 nM
 Bioactivity Info 
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CP0431716
 Compound Info 
9-(2-chlorobenzoyl)-N-[4-(trifluoromethyl)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C23H23ClF3N3O3
  1
1
IC50 = 13 nM
 Bioactivity Info 
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CP0471434
 Compound Info 
9-(cyclohexanecarbonyl)-N-[4-(trifluoromethoxy)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C23H30F3N3O4
  1
1
IC50 = 14.5 nM
 Bioactivity Info 
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CP0430058
 Compound Info 
9-(4-cyanobenzoyl)-N-[4-(trifluoromethyl)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C24H23F3N4O3
  1
1
IC50 = 15 nM
 Bioactivity Info 
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CP0339288
 Compound Info 
8-(2,2-difluorocyclopropanecarbonyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C20H22F5N3O3
  1
1
IC50 = 15.8 nM
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CP0443468
 Compound Info 
9-(2,6-difluorobenzoyl)-N-[4-(trifluoromethoxy)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C23H22F5N3O4
  1
1
IC50 = 18.5 nM
 Bioactivity Info 
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CP0439869
 Compound Info 
9-(2,6-difluorobenzoyl)-N-[4-(trifluoromethyl)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C23H22F5N3O3
  1
1
IC50 = 19.1 nM
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CP0175591
 Compound Info 
8-(1H-benzimidazole-4-carbonyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C24H24F3N5O3
  1
1
IC50 = 22.2 nM
 Bioactivity Info 
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CP0467266
 Compound Info 
9-(5-cyclopropyl-1,2-oxazole-4-carbonyl)-N-[4-(trifluoromethoxy)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C23H25F3N4O5
  1
1
IC50 = 22.2 nM
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CP0224939
 Compound Info 
8-(4-hydroxycyclohexanecarbonyl)-N-[4-(trifluoromethoxy)phenyl]-2,8-diazaspiro[4.5]decane-2-carboxamide
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C23H30F3N3O4
  1
1
IC50 = 22.4 nM
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CP0421106
 Compound Info 
9-(4-chlorobenzoyl)-N-[4-(trifluoromethyl)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C23H23ClF3N3O3
  1
1
IC50 = 24.9 nM
 Bioactivity Info 
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CP0439868
 Compound Info 
9-(5-methyl-1,2-oxazole-3-carbonyl)-N-[4-(trifluoromethoxy)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C21H23F3N4O5
  1
1
IC50 = 25.7 nM
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CP0430055
 Compound Info 
9-(1-phenylcyclopropanecarbonyl)-N-[4-(trifluoromethoxy)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C26H28F3N3O4
  1
1
IC50 = 28 nM
 Bioactivity Info 
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CP0390071
 Compound Info 
9-(2-cyclopropylacetyl)-N-[4-(trifluoromethyl)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C21H26F3N3O3
  1
1
IC50 = 31.4 nM
 Bioactivity Info 
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CP0390073
 Compound Info 
9-(3-chlorobenzoyl)-N-[4-(trifluoromethyl)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C23H23ClF3N3O3
  1
1
IC50 = 36.9 nM
 Bioactivity Info 
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CP0421089
 Compound Info 
9-[2-(2-methoxyphenyl)acetyl]-N-[4-(trifluoromethoxy)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C25H28F3N3O5
  1
1
IC50 = 71.6 nM
 Bioactivity Info 
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CP0421091
 Compound Info 
9-(5-cyclopropyl-1H-pyrazole-4-carbonyl)-N-[4-(trifluoromethoxy)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C23H26F3N5O4
  1
1
IC50 = 96.9 nM
 Bioactivity Info 
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CP0430054
 Compound Info 
9-(2-pyridin-2-ylacetyl)-N-[4-(trifluoromethoxy)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C23H25F3N4O4
  1
1
IC50 > 20 nM
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CP0431713
 Compound Info 
9-(2-oxo-1,3-dihydroindole-5-carbonyl)-N-[4-(trifluoromethoxy)phenyl]-1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxamide
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C25H25F3N4O5
  1
1
IC50 > 20 nM
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Similar Protein(s) and Bioactivity Statistics
90% Identity
Protein ID Protein Name Protein Organism
PT01527 Bifunctional epoxide hydrolase 2 Mus musculus, Mouse
 Protein Info 
50% Identity
Protein ID Protein Name Protein Organism
PT01103 Bifunctional epoxide hydrolase 2 Homo sapiens, Human
 Protein Info