Bioactivity Information

Brief Information of the Compound and Protein

Compound Name

Compound Info

6alpha-ethyl-23(S)-methyl-cholic acid

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PubChem ID

CID: 45483949

Canonical SMILES	CC[C@H]1[C@@H](O)[C@H]2[C@@H]3CC[C@H]([C@H](C)C[C@H](C)C(O)=O)[C@@]3(C)[C@@H](O)C[C@@H]2[C@@]2(C)CC[C@@H](O)C[C@@H]12 Show/Hide
Compound Formula	C27H46O5

Compound Structure

Protein Name

Protein Info

G-protein coupled bile acid receptor 1

Protein Classification

Membrane receptor

Family A G protein-coupled receptor

Small molecule receptor (family A GPCR)

Lipid-like ligand receptor (family A GPCR)

Steroid-like ligand receptor

Uniprot ID

Q8TDU6

Gene Name

GPBAR1

Table of Molecular Bioactivities Related to the Compound and Protein

Cell-based Assay

Cell Line ID	Cell Line Name	Cell Line Organism
CL000011	CHO Cell Line Info	Cricetulus griseus (Chinese hamster)	1
1	EC50 = 3440 nM	TI LI LO TS
CL000386	Flp-In-CHO Cell Line Info	Cricetulus griseus (Chinese hamster)	2
1	EC50 = 166 nM	TI LI LO TS
2	EC50 = 5540 nM	TI LI LO TS
CL000025	HEK-293T Cell Line Info	Homo sapiens (Human)	1
1	EC50 = 820 nM	TI LI LO TS
CL000006	HEK293 Cell Line Info	Homo sapiens (Human)	4
1	EC50 = 95.8 nM	TI LI LO TS
2	EC50 = 293 nM	TI LI LO TS
3	EC50 = 1004 nM	TI LI LO TS
4	EC50 = 1230 nM	TI LI LO TS
CL000126	NCI-H716 Cell Line Info	Homo sapiens (Human)	3
1	EC50 = 820 nM	TI LI LO TS
2	EC50 = 900 nM	TI LI LO TS
3	EC50 = 13000 nM	TI LI LO TS

Biochemical Assays

1	EC50 = 180 nM