General Information of the Protein
Protein ID
PT03572
Protein Name
Methionine synthase
Secondarily
Protein Name
5-methyltetrahydrofolate--homocysteine methyltransferase
Cobalamin-dependent methionine synthase
Vitamin-B12 dependent methionine synthase
Gene Name
MTR
Sequence
MSPALQDLSQPEGLKKTLRDEINAILQKRIMVLDGGMGTMIQREKLNEEHFRGQEFKDHARPLKGNNDILSITQPDVIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAEEVTLQTGIKRFVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYQEQAKGLLDGGVDILLIETIFDTANAKAALFALQNLFEEKYAPRPIFISGTIVDKSGRTLSGQTGEGFVISVSHGEPLCIGLNCALGAAEMRPFIEIIGKCTTAYVLCYPNAGLPNTFGDYDETPSMMAKHLKDFAMDGLVNIVGGCCGSTPDHIREIAEAVKNCKPRVPPATAFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRKFAKLIMAGNYEEALCVAKVQVEMGAQVLDVNMDDGMLDGPSAMTRFCNLIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKYGAAMVVMAFDEEGQATETDTKIRVCTRAYHLLVKKLGFNPNDIIFDPNILTIGTGMEEHNLYAINFIHATKVIKETLPGARISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKSGMDMGIVNAGNLPVYDDIHKELLQLCEDLIWNKDPEATEKLLRYAQTQGTGGKKVIQTDEWRNGPVEERLEYALVKGIEKHIIEDTEEARLNQKKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETRVLNGTVEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIRIPLLIGGATTSKTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFEEIMEEYEDIRQDHYESLKERRYLPLSQARKSGFQMDWLSEPHPVKPTFIGTQVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKTVGGEARKVYDDAHNMLNTLISQKKLRARGVVGFWPAQSIQDDIHLYAEAAVPQAAEPIATFYGLRQQAEKDSASTEPYYCLSDFIAPLHSGIRDYLGLFAVACFGVEELSKAYEDDGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYCGSEQLDVADLRRLRYKGIRPAPGYPSQPDHTEKLTMWRLADIEQSTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQVEDYALRKNISVAEVEKWLGPILGYDTD
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Organism
Homo sapiens, Human
Protein Classification
Enzyme
>
Transferase
Function
Catalyzes the transfer of a methyl group from methylcob(III)alamin (MeCbl) to homocysteine, yielding enzyme-bound cob(I)alamin and methionine in the cytosol (PubMed:16769880, PubMed:27771510, PubMed:17288554). MeCbl is an active form of cobalamin (vitamin B12) used as a cofactor for methionine biosynthesis. Cob(I)alamin form is regenerated to MeCbl by a transfer of a methyl group from 5-methyltetrahydrofolate (PubMed:16769880, PubMed:27771510, PubMed:17288554). The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR (methionine synthase reductase) and MTR which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine (PubMed:16769880, PubMed:27771510).
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Uniprot ID
Primary ID:
Q99707

Secondarily ID:
A1L4N8
A9Z1W4
B7ZLW7
B9EGF7
Q99713
Q99723
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Ensembl ID
ENSG00000116984
HGNC ID
HGNC:7468
Subcellular Location
Cytoplasm
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000018 , HL-60
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0499743
 Compound Info 
(2S)-2-[[3-bromo-4-[[2,4-diamino-5-(2,3-dibromopropyl)-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-6-yl]methylamino]benzoyl]amino]pentanedioic acid
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C23H28Br3N7O5
  1
1
IC50 = 1430 nM
 Bioactivity Info 
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CP0335321
 Compound Info 
(2S)-2-[[4-[acetyl-[(2,4-diamino-5-formyl-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-6-yl)methyl]amino]benzoyl]amino]pentanedioic acid
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C23H27N7O7
  1
1
IC50 = 1730 nM
 Bioactivity Info 
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CP0533498
 Compound Info 
2-chloro-1-[2,4-diamino-6-[2-(4-ethylphenyl)ethyl]-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-5-yl]ethanone
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C19H24ClN5O
  3
1
IC50 = 4460 nM
 Bioactivity Info 
   TI
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2
IC50 = 7640 nM
 Bioactivity Info 
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3
IC50 = 12100 nM
 Bioactivity Info 
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CP0527717
 Compound Info 
2-chloro-1-[2,4-diamino-6-[2-(4-fluorophenyl)ethyl]-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-5-yl]ethanone
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C17H19ClFN5O
  1
1
IC50 = 4990 nM
 Bioactivity Info 
   TI
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CP0553019
 Compound Info 
2-chloro-1-[2,4-diamino-6-[2-(4-methylphenyl)ethyl]-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-5-yl]ethanone
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C18H22ClN5O
  1
1
IC50 = 5660 nM
 Bioactivity Info 
   TI
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CP0194830
 Compound Info 
(2S)-2-[[4-[(2,4-diamino-5-formyl-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid
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C22H27N7O6
  1
1
IC50 = 8110 nM
 Bioactivity Info 
   TI
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CP0560734
 Compound Info 
2-chloro-1-[2,4-diamino-6-[2-(4-tert-butylphenyl)ethyl]-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-5-yl]ethanone
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C21H28ClN5O
  1
1
IC50 = 13500 nM
 Bioactivity Info 
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CP0570005
 Compound Info 
2-chloro-1-[2,4-diamino-6-[2-(4-chlorophenyl)ethyl]-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-5-yl]ethanone
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C17H19Cl2N5O
  1
1
IC50 = 14900 nM
 Bioactivity Info 
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CP0450144
 Compound Info 
(2S)-2-[[4-[(2,4-diamino-5-prop-2-enyl-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-6-yl)methyl-prop-2-enylamino]benzoyl]amino]pentanedioic acid
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C26H33N7O5
  1
1
IC50 = 15870 nM
 Bioactivity Info 
   TI
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CP0493586
 Compound Info 
(2S)-2-[[3-bromo-4-[(2,4-diamino-5-prop-2-enyl-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-6-yl)methylamino]benzoyl]amino]pentanedioic acid
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C23H28BrN7O5
  1
1
IC50 = 21000 nM
 Bioactivity Info 
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CP0534353
 Compound Info 
(2S)-2-[[4-[(2,4-diamino-5-prop-2-enyl-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-6-yl)methylamino]benzoyl]amino]pentanedioic acid
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C23H29N7O5
  1
1
IC50 = 27830 nM
 Bioactivity Info 
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CP0542490
 Compound Info 
2-chloro-1-[2,4-diamino-6-[2-(4-propan-2-ylphenyl)ethyl]-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-5-yl]ethanone
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C20H26ClN5O
  1
1
IC50 = 45900 nM
 Bioactivity Info 
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CP0572576
 Compound Info 
2-bromo-1-[2,4-diamino-6-[2-(4-methylphenyl)ethyl]-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-5-yl]ethanone
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C18H22BrN5O
  1
1
IC50 = 48000 nM
 Bioactivity Info 
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CP0560735
 Compound Info 
2-chloro-1-[2,4-diamino-6-[2-(2-methoxyphenyl)ethyl]-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-5-yl]ethanone
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C18H22ClN5O2
  1
1
IC50 = 51500 nM
 Bioactivity Info 
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CP0260606
 Compound Info 
(2S)-2-[[4-[[2,4-diamino-5-(4-methylphenyl)sulfonyl-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-6-yl]methyl-methylamino]benzoyl]amino]pentanedioic acid
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C28H33N7O7S
  1
1
IC50 = 55000 nM
 Bioactivity Info 
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CP0300787
 Compound Info 
(2S)-2-[[4-(11,13-diamino-2,4,10,12-tetrazatricyclo[7.4.0.02,6]trideca-1(9),10,12-trien-4-yl)benzoyl]amino]pentanedioic acid
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C21H25N7O5
  1
1
IC50 = 66380 nM
 Bioactivity Info 
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CP0525893
 Compound Info 
(2S)-2-[[4-[(2,4-diamino-5,6,7,8-tetrahydropyrido[3,2-d]pyrimidin-6-yl)methylamino]benzoyl]amino]pentanedioic acid
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C20H25N7O5
  1
1
IC50 = 87910 nM
 Bioactivity Info 
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CP0057302
 Compound Info 
(2S)-2-[[4-[(2,4-diaminopyrido[3,2-d]pyrimidin-6-yl)methylamino]benzoyl]amino]pentanedioic acid
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C20H21N7O5
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0194829
 Compound Info 
(2S)-2-[[4-[(2,4-diamino-5,6,7,8-tetrahydropyrido[3,2-d]pyrimidin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid
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C21H27N7O5
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0339485
 Compound Info 
(2S)-2-[[4-[(2,4-diamino-5-prop-2-enyl-7,8-dihydro-6H-pyrido[3,2-d]pyrimidin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid
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C24H31N7O5
  1
1
IC50 > 100000 nM
 Bioactivity Info 
   TI
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CP0339778
 Compound Info 
(2S)-2-[[4-[acetyl-[(2,4-diamino-5,6,7,8-tetrahydropyrido[3,2-d]pyrimidin-6-yl)methyl]amino]benzoyl]amino]pentanedioic acid
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C22H27N7O6
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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Clinical Information about the Protein
Target 1 ( Methionine synthase (MTR) )
Target Type Literature-reported Target