General Information of the Protein
Protein ID
PT03321
Protein Name
17-beta-hydroxysteroid dehydrogenase type 3
Secondarily
Protein Name
Estradiol 17-beta-dehydrogenase 2
Short chain dehydrogenase/reductase family 12C member 2
Testicular 17-beta-hydroxysteroid dehydrogenase
Testosterone 17-beta-dehydrogenase 3
Gene Name
HSD17B3
Secondarily
Gene Name
EDH17B3
SDR12C2
Sequence
MGDVLEQFFILTGLLVCLACLAKCVRFSRCVLLNYWKVLPKSFLRSMGQWAVITGAGDGIGKAYSFELAKRGLNVVLISRTLEKLEAIATEIERTTGRSVKIIQADFTKDDIYEHIKEKLAGLEIGILVNNVGMLPNLLPSHFLNAPDEIQSLIHCNITSVVKMTQLILKHMESRQKGLILNISSGIALFPWPLYSMYSASKAFVCAFSKALQEEYKAKEVIIQVLTPYAVSTAMTKYLNTNVITKTADEFVKESLNYVTIGGETCGCLAHEILAGFLSLIPAWAFYSGAFQRLLLTHYVAYLKLNTKVR
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Organism
Homo sapiens, Human
Protein Classification
Enzyme
>
Oxidoreductase
Function
Catalyzes the conversion of 17-oxosteroids to 17beta-hydroxysteroids (PubMed:8075637, PubMed:16216911, PubMed:27927697, PubMed:26545797). Favors the reduction of androstenedione to testosterone (PubMed:16216911, PubMed:27927697, PubMed:26545797). Testosterone is the key androgen driving male development and function (PubMed:8075637). Uses NADPH while the two other EDH17B enzymes use NADH (PubMed:26545797, PubMed:8075637, PubMed:16216911). Androgens such as epiandrosterone, dehydroepiandrosterone, androsterone and androstanedione are accepted as substrates and reduced at C-17 (PubMed:16216911). Can reduce 11-ketoandrostenedione as well as 11beta-hydroxyandrostenedione at C-17 to the respective testosterone forms (PubMed:16216911, PubMed:27927697).
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Uniprot ID
Primary ID:
P37058

Secondarily ID:
Q5U0Q6
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Ensembl ID
ENSG00000130948
HGNC ID
HGNC:5212
Subcellular Location
Endoplasmic reticulum
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000006 , HEK293
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0386569
 Compound Info 
(3R,8R,9S,10S,13S,14S)-3-benzyl-3-hydroxy-10,13-dimethyl-2,4,5,6,7,8,9,11,12,14,15,16-dodecahydro-1H-cyclopenta[a]phenanthren-17-one
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C26H36O2
  1
1
IC50 = 57 nM
 Bioactivity Info 
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CP0392643
 Compound Info 
(3R,8R,9S,10S,13S,14S)-3-(2-cyclohexylethyl)-3-hydroxy-10,13-dimethyl-2,4,5,6,7,8,9,11,12,14,15,16-dodecahydro-1H-cyclopenta[a]phenanthren-17-one
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C27H44O2
  1
1
IC50 = 60 nM
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CP0430648
 Compound Info 
(3R,8R,9S,10S,13S,14S)-3-hydroxy-10,13-dimethyl-3-propyl-2,4,5,6,7,8,9,11,12,14,15,16-dodecahydro-1H-cyclopenta[a]phenanthren-17-one
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C22H36O2
  1
1
IC50 = 67 nM
 Bioactivity Info 
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CP0378689
 Compound Info 
(3R,8R,9S,10S,13S,14S)-3-butan-2-yl-3-hydroxy-10,13-dimethyl-2,4,5,6,7,8,9,11,12,14,15,16-dodecahydro-1H-cyclopenta[a]phenanthren-17-one
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C23H38O2
  1
1
IC50 = 73 nM
 Bioactivity Info 
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CP0847904
 Compound Info 
(3R,10S,13S)-3-Methoxy-10,13-dimethyl-3-phenethyl-hexadecahydro-cyclopenta[a]phenanthren-17-one
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C28H40O2
  1
1
IC50 = 73 nM
 Bioactivity Info 
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CP0405802
 Compound Info 
(3R,8R,9S,10S,13S,14S)-3-hydroxy-10,13-dimethyl-3-phenyl-2,4,5,6,7,8,9,11,12,14,15,16-dodecahydro-1H-cyclopenta[a]phenanthren-17-one
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C25H34O2
  1
1
IC50 = 81 nM
 Bioactivity Info 
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CP0406436
 Compound Info 
(3S,8R,9S,10S,13S,14S)-3-(cyclohexylmethyl)-3-hydroxy-10,13-dimethyl-2,4,5,6,7,8,9,11,12,14,15,16-dodecahydro-1H-cyclopenta[a]phenanthren-17-one
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C26H42O2
  1
1
IC50 = 87 nM
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CP0372629
 Compound Info 
(3R,8R,9S,10S,13S,14S)-3-cyclohexyl-3-hydroxy-10,13-dimethyl-2,4,5,6,7,8,9,11,12,14,15,16-dodecahydro-1H-cyclopenta[a]phenanthren-17-one
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C25H40O2
  1
1
IC50 = 97 nM
 Bioactivity Info 
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CP0404703
 Compound Info 
(3R,8R,9S,10S,13S,14S)-3-hydroxy-10,13-dimethyl-3-(2-phenylethyl)-2,4,5,6,7,8,9,11,12,14,15,16-dodecahydro-1H-cyclopenta[a]phenanthren-17-one
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C27H38O2
  1
1
IC50 = 99 nM
 Bioactivity Info 
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CP0293004
 Compound Info 
(3R,8R,9S,10S,13S,14S)-3-hexyl-3-hydroxy-10,13-dimethyl-2,4,5,6,7,8,9,11,12,14,15,16-dodecahydro-1H-cyclopenta[a]phenanthren-17-one
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C25H42O2
  1
1
IC50 = 100 nM
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CP0851303
 Compound Info 
(3R,10S,13S)-3-Butyl-3-hydroxy-10,13-dimethyl-hexadecahydro-cyclopenta[a]phenanthren-17-one
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C23H38O2
  1
1
IC50 = 116 nM
 Bioactivity Info 
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CP0853920
 Compound Info 
(3R,10S,13S)-3-tert-Butyl-3-hydroxy-10,13-dimethyl-hexadecahydro-cyclopenta[a]phenanthren-17-one
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C23H38O2
  1
1
IC50 = 142 nM
 Bioactivity Info 
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CP0378928
 Compound Info 
(3R,8R,9S,10S,13S,14S)-3-hydroxy-10,13-dimethyl-3-octyl-2,4,5,6,7,8,9,11,12,14,15,16-dodecahydro-1H-cyclopenta[a]phenanthren-17-one
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C27H46O2
  1
1
IC50 = 147 nM
 Bioactivity Info 
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CP0895652
 Compound Info 
(3R,10S,13S)-3-Benzyl-3-methoxy-10,13-dimethyl-hexadecahydro-cyclopenta[a]phenanthren-17-one
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C27H38O2
  1
1
IC50 = 154 nM
 Bioactivity Info 
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CP0063737
 Compound Info 
(3alpha,5beta)-3-hydroxyandrostan-17-one
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C19H30O2
  1
1
IC50 = 330 nM
 Bioactivity Info 
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CP0847902
 Compound Info 
(3R,10S,13S)-3-Benzyl-3-ethoxy-10,13-dimethyl-hexadecahydro-cyclopenta[a]phenanthren-17-one
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C28H40O2
  1
1
IC50 = 352 nM
 Bioactivity Info 
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CP0881037
 Compound Info 
(3R,10S,13S)-3-(2-Cyclohexyl-ethyl)-3-methoxy-10,13-dimethyl-hexadecahydro-cyclopenta[a]phenanthren-17-one
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C28H46O2
  1
1
IC50 = 354 nM
 Bioactivity Info 
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CP0038806
 Compound Info 
Androst-4-en-3,17-dione, 2
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C19H26O2
  1
1
IC50 = 758 nM
 Bioactivity Info 
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Cell Line ID: CL000017 , HeLa
 Cell Line Info 
Cell Line ID: CL000141 , LNCaP
 Cell Line Info