Protein Information

General Information of the Protein

Protein ID	PT01472
Protein Name	Nitric oxide synthase, inducible
Secondarily Protein Name	Hepatocyte NOS Inducible NO synthase NOS type II Peptidyl-cysteine S-nitrosylase NOS2
Gene Name	NOS2
Secondarily Gene Name	NOS2A
Sequence	MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVRLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLQKLAQVATEEPERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPSSLEMSAL Show/Hide
Organism	Homo sapiens, Human
Protein Classification	Enzyme
Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004, PubMed:7682706, PubMed:7504305). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed:19688109). Show/Hide
Uniprot ID	Primary ID: P35228 Secondarily ID: A1L3U5 B7ZLY2 O60757 O94994 Q16263 Q16692 Q4TTS5 Q9UD42 Show/Hide
Ensembl ID	ENSG00000007171
HGNC ID	HGNC:7873
Subcellular Location	Cytoplasm Cytosol

Map of Molecular Bioactivity Related to the Protein

Map of Molecular Bioactivity Related to the Protein Protein Cell Line Compound Bioactivity Value: <= 0.1 μM > 0.1 μM and <= 10 μM > 10 μM Imprecise Activity

Table of Molecular Bioactivities Related to the Protein

Cell Line ID: CL000068 , A-549

Cell Line Info

Compound ID	Compound Name	Compound Formula
CP0467903 Compound Info	6-Allyl-4-methyl-5,6-dihydro-1H-pyridin-(2Z)-ylideneamine Show/Hide	C9H14N2	1
1	IC50 = 50 nM Bioactivity Info	TI LI LO TS
CP0403656 Compound Info	4-Methyl-6-propyl-5,6-dihydro-1H-pyridin-(2Z)-ylideneamine Show/Hide	C9H16N2	1
1	IC50 = 60 nM Bioactivity Info	TI LI LO TS
CP0475659 Compound Info	(4aR,7aR)-4-Methyl-1,4a,5,6,7,7a-hexahydro-[1]pyrindin-(2E)-ylideneamine Show/Hide	C9H14N2	1
1	IC50 = 170 nM Bioactivity Info	TI LI LO TS
CP0220714 Compound Info	4,6-Dimethyl-5,6-dihydro-1H-pyridin-(2Z)-ylideneamine Show/Hide	C7H12N2	1
1	IC50 = 180 nM Bioactivity Info	TI LI LO TS
CP0438146 Compound Info	4-Methyl-3,6-dihydro-1H-pyridin-(2Z)-ylideneamine Show/Hide	C6H10N2	1
1	IC50 = 200 nM Bioactivity Info	TI LI LO TS
CP0401142 Compound Info	3,4-Dimethyl-5,6-dihydro-1H-pyridin-(2Z)-ylideneamine Show/Hide	C7H12N2	1
1	IC50 = 310 nM Bioactivity Info	TI LI LO TS
CP0461265 Compound Info	4,5,5-Trimethyl-5,6-dihydro-1H-pyridin-(2Z)-ylideneamine Show/Hide	C8H14N2	1
1	IC50 = 420 nM Bioactivity Info	TI LI LO TS
CP0222909 Compound Info	4,5-Dimethyl-5,6-dihydro-1H-pyridin-(2Z)-ylideneamine Show/Hide	C7H12N2	1
1	IC50 = 510 nM Bioactivity Info	TI LI LO TS
CP0900316 Compound Info	4-Methyl-3,6-dihydro-1H-pyridin-(2Z)-ylideneamine Show/Hide	C6H10N2	1
1	IC50 = 700 nM Bioactivity Info	TI LI LO TS
CP0255072 Compound Info	4-Ethyl-5,6-dihydro-1H-pyridin-(2Z)-ylideneamine Show/Hide	C7H12N2	1
1	IC50 = 1200 nM Bioactivity Info	TI LI LO TS
CP0408591 Compound Info	(4aR,8aR)-4-Methyl-4a,5,6,7,8,8a-hexahydro-1H-quinolin-(2E)-ylideneamine Show/Hide	C10H16N2	1
1	IC50 = 1400 nM Bioactivity Info	TI LI LO TS
CP0436578 Compound Info	5,5-Dimethyl-5,6-dihydro-1H-pyridin-(2Z)-ylideneamine Show/Hide	C7H12N2	1
1	IC50 = 2800 nM Bioactivity Info	TI LI LO TS

Cell Line ID: CL000438 , A-172

Cell Line Info

Cell Line ID: CL000214 , BV-2

Cell Line Info

Cell Line ID: CL000134 , DLD-1

Cell Line Info

Cell Line ID: CL000006 , HEK293

Cell Line Info

Cell Line ID: CL000013 , Sf9

Cell Line Info

Cell Line ID: CL000040 , THP-1

Cell Line Info

Biochemical Assays

Compound ID	Compound Name	Compound Formula
CP0161026 Compound Info	3-{[(Benzo[1,3]dioxol-5-ylmethyl)-carbamoyl]-methyl}-4-(2-imidazol-1-yl-pyrimidin-4-yl)-piperazine-1-carboxylic acid methyl ester Show/Hide	C23H25N7O5	2
1	IC50 = 0.6 nM
2	Ki = 2.2 nM
CP0655512 Compound Info	(R)-2-(3-amino-1-phenylpropoxy)-4-chlorobenzonitrile oxalate Show/Hide	C18H17ClN2O5	1
1	IC50 = 4 nM
CP0758452 Compound Info	(R)-2-(3-amino-1-phenylpropylthio)-6-methylnicotinonitrile fumarate Show/Hide	C20H21N3O4S	1
1	IC50 = 4 nM
CP0762448 Compound Info	(R)-2-(3-amino-1-phenylpropylthio)-4-chlorobenzonitrile fumarate Show/Hide	C20H19ClN2O4S	1
1	IC50 = 4 nM
CP0742608 Compound Info	(R)-2-(3-amino-1-(isoxazol-5-yl)propoxy)-6-(trifluoromethyl)nicotinonitrile oxalate Show/Hide	C15H13F3N4O6	1
1	IC50 = 5.4 nM
CP0742609 Compound Info	2-[[(1R)-3-Amino-1-phenylpropyl]oxy]-4-chloro-5-fluorobenzonitrile oxalate Show/Hide	C18H16ClFN2O5	1
1	IC50 = 6 nM
CP0338436 Compound Info	N-(1,3-benzodioxol-5-ylmethyl)-N'-(3-imidazol-1-yl-1,2,4-thiadiazol-5-yl)-N'-methylpropane-1,3-diamine Show/Hide	C17H20N6O2S	1
1	IC50 = 7 nM
CP0223802 Compound Info	N'-[[3-(aminomethyl)phenyl]methyl]ethanimidamide Show/Hide	C10H15N3	1
1	IC50 = 8.4 nM
CP0655513 Compound Info	(R)-4-chloro-2-(3-(methylamino)-1-phenylpropoxy)benzonitrile hydrochloride Show/Hide	C17H18Cl2N2O	1
1	IC50 = 9 nM
CP0089375 Compound Info	quinolinone, 12 Show/Hide	C21H15ClFN3O2S	1
1	IC50 = 11 nM
CP0742605 Compound Info	(R)-3-(2,5-dichlorophenoxy)-N-methyl-3-phenylpropan-1-amine fumarate Show/Hide	C20H21Cl2NO5	1
1	IC50 = 22 nM
CP0046958 Compound Info	1-(6-CYANO-3-PYRIDYLCARBONYL)-5',8'-DIFLUOROSPIRO[PIPERIDINE-4,2'(1'H)-QUINAZOLINE]-4'-AMINE Show/Hide	C19H16F2N6O	5
1	IC50 = 35 nM
2	IC50 = 37 nM
3	IC50 = 37.15 nM
4	IC50 = 41 nM
5	IC50 = 900 nM
CP0742607 Compound Info	rac-4-chloro-2-(1-(methylamino)pentan-3-yloxy)benzonitrile hydrochloride Show/Hide	C13H18Cl2N2O	1
1	IC50 = 51 nM
CP0042227 Compound Info	(S)-4-(2-Acetimidoylamino-ethylsulfanyl)-2-amino-butyric acid Show/Hide	C8H17N3O2S	3
1	IC50 = 70 nM
2	IC50 = 1400 nM
3	IC50 = 1412.54 nM
CP0742610 Compound Info	(R)-2-(3-(methylamino)-1-phenylpropoxy)-4-(trifluoromethyl)benzonitrile hydrochloride Show/Hide	C18H18ClF3N2O	1
1	IC50 = 71 nM
CP0044041 Compound Info	(L-N6-1-iminoethyl)lysine Show/Hide	C8H17N3O2	8
1	IC50 = 100 nM
2	IC50 = 130 nM
3	IC50 = 1400 nM
4	IC50 = 2100 nM
5	IC50 = 4600 nM
6	IC50 = 4677.35 nM
7	IC50 = 4900 nM
8	IC50 = 5000 nM
CP0769297 Compound Info	(R)-4-methoxy-2-(3-(methylamino)-1-phenylpropoxy)benzonitrile oxalate Show/Hide	C20H22N2O6	1
1	IC50 = 110 nM
CP0743881 Compound Info	S-[2-(1-Iminoethylamino)ethyl]-2-methyl-(D/L)-cysteine bistrifluoroacetate Show/Hide	C11H18F6N4O6S	1
1	IC50 = 700 nM
CP0746845 Compound Info	S-[2-[(1-Iminoethyl)aminolethyl]-2-methyl-L-cysteine dihydrochloride Show/Hide	C7H18Cl2N4O2S	1
1	IC50 = 700 nM
CP0053263 Compound Info	(2S)-2-azaniumyl-5-[(N'-methylcarbamimidoyl)amino]pentanoate Show/Hide	C7H16N4O2	7
1	IC50 = 1000 nM
2	IC50 = 3400 nM
3	IC50 = 6600 nM
4	IC50 = 10000 nM
5	IC50 = 14000 nM
6	IC50 = 27000 nM
7	Ki = 860 nM
CP0255072 Compound Info	4-Ethyl-5,6-dihydro-1H-pyridin-(2Z)-ylideneamine Show/Hide	C7H12N2	1
1	IC50 = 1200 nM
CP0055850 Compound Info	Aminoguanidine Show/Hide	CH6N4	1
1	IC50 = 3900 nM
CP0047550 Compound Info	7-nitro-1H-indazole Show/Hide	C7H5N3O2	1
1	IC50 = 5800 nM
CP0043950 Compound Info	(S)-methyl 2-amino-5-(amino(nitroamino)methyleneamino)pentanoate Show/Hide	C7H15N5O4	2
1	IC50 = 8000 nM
2	IC50 = 14000 nM
CP0888278 Compound Info	2-[[[2-[(1-Iminoethyl)amino]ethyllthiolmethyl]-O-methyl-D-serine dihydrochloride Show/Hide	C8H20Cl2N4O3S	1
1	IC50 = 8200 nM
CP0052287 Compound Info	(2S)-5-{[(Z)-amino(imino)methyl]amino}-2-(methylamino)pentanoic acid Show/Hide	C7H16N4O2	1
1	IC50 = 19000 nM
CP0036225 Compound Info	N-benzyl-N'-(3-imidazol-1-yl-1,2,4-thiadiazol-5-yl)-N'-methylpropane-1,3-diamine Show/Hide	C16H20N6S	1
1	IC50 > 100000 nM
CP0040036 Compound Info	(S)-5-Acetimidoylamino-2-amino-pent Show/Hide	C7H15N3O2	1
1	Ki = 340 nM

Clinical Information about the Protein

Target 1 ( Nitric-oxide synthase inducible (NOS2) )

Target Type	Clinical trial Target
Disease	6 Target-related Diseases				6
1	Solid tumour/cancer [ICD-11: 2A00-2F9Z]
2	Diabetic kidney disease [ICD-11: GB61.Z]
3	Asthma [ICD-11: CA23]
4	Diabetic retinopathy [ICD-11: 9B71.0]
5	Sepsis [ICD-11: 1G40-1G41]
6	Inflammation [ICD-11: 1A00-CA43.1]
Clinical Trial Drug(s)	4 Clinical Trial Drugs				4
1	Curcumin	Drug Info	Phase 3	Solid tumour/cancer
2	Pimagedine HCl	Drug Info	Phase 2/3	Diabetic kidney disease
3	GW274150	Drug Info	Phase 2	Asthma
4	Aminoguanidine	Drug Info	Phase 1	Diabetic retinopathy
Discontinued Drug(s)	1 Discontinued Drug				1
1	ONO-1714	Drug Info	Discontinued in Phase 1	Sepsis
Investigative Drug(s)	2 Investigative Drugs				2
1	BYK-191023	Drug Info	Investigative	Inflammation
2	SKLB-010	Drug Info	Investigative	Inflammation

Similar Protein(s) and Bioactivity Statistics

50% Identity

Protein ID	Protein Name	Protein Organism
PT01718	Nitric oxide synthase, inducible	Mus musculus, Mouse	Protein Info

Cite

About MolBiC

Correspondence

Prof. Feng ZHU

Prof. Leo, Lianyi Han