General Information of the Protein
Protein ID
PT01234
Protein Name
Macrophage migration inhibitory factor
Secondarily
Protein Name
Glycosylation-inhibiting factor
L-dopachrome isomerase
L-dopachrome tautomerase
Phenylpyruvate tautomerase
Gene Name
MIF
Secondarily
Gene Name
GLIF
MMIF
Sequence
MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA
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Organism
Homo sapiens, Human
Protein Classification
Enzyme
Function
Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (PubMed:15908412, PubMed:17443469, PubMed:23776208). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (PubMed:15908412, PubMed:17443469, PubMed:23776208). Counteracts the anti-inflammatory activity of glucocorticoids (PubMed:15908412, PubMed:17443469, PubMed:23776208). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed:11439086, PubMed:17526494). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed:11439086, PubMed:17526494).
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Uniprot ID
Primary ID:
P14174

Secondarily ID:
A5Z1R8
B2R4S3
Q2V4Y5
Q6FHV0
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Ensembl ID
ENSG00000240972
HGNC ID
HGNC:7097
Subcellular Location
Secreted
Cytoplasm
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000001 , Jurkat
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0041478
 Compound Info 
3-isothiocyanatoprop-1-ene
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C4H5NS
  1
1
IC50 = 250 nM
 Bioactivity Info 
   TI
   LI
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CP0463220
 Compound Info 
4-(isothiocyanatomethyl)benzenesulfonamide
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C8H8N2O2S2
  1
1
IC50 = 500 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0105333
 Compound Info 
(isothiocyanatomethyl)benzene
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C8H7NS
  1
1
IC50 = 540 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0396355
 Compound Info 
1-fluoro-4-(isothiocyanatomethyl)benzene
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C8H6FNS
  2
1
IC50 = 540 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
IC50 = 9600 nM
 Bioactivity Info 
   TI
   LI
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CP0456779
 Compound Info 
(R)-(1-Isothiocyanatoethyl)Benzene
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C9H9NS
  1
1
IC50 = 1100 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0499958
 Compound Info 
1-(1-isothiocyanatopropyl)-4-methoxybenzene
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C11H13NOS
  1
1
IC50 = 1200 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0512566
 Compound Info 
4-(2-isothiocyanatoethyl)phenol
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C9H9NOS
  1
1
IC50 = 1200 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0396356
 Compound Info 
(3-Isothiocyanatopropyl)Benzene
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C10H11NS
  1
1
IC50 = 1400 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0459785
 Compound Info 
1-fluoro-3-(isothiocyanatomethyl)benzene
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C8H6FNS
  2
1
IC50 = 1500 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
IC50 = 11000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0000476
 Compound Info 
(2-Isothiocyanato-ethyl)-benzene
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C9H9NS
  1
1
IC50 = 1600 nM
 Bioactivity Info 
   TI
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CP0459786
 Compound Info 
1-chloro-4-(2-isothiocyanatoethyl)benzene
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C9H8ClNS
  1
1
IC50 = 1600 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0487613
 Compound Info 
1-(2-isothiocyanatoethoxy)-2-methoxybenzene
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C10H11NO2S
  1
1
IC50 = 1800 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0452248
 Compound Info 
Phenylbutyl Isothiocyanate
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C11H13NS
  1
1
IC50 = 1900 nM
 Bioactivity Info 
   TI
   LI
   LO
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CP0055024
 Compound Info 
4-(Methylsulfinyl)Butyl Isothiocyanate
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C6H11NOS2
  1
1
IC50 = 2200 nM
 Bioactivity Info 
   TI
   LI
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CP0448381
 Compound Info 
1-PHENYLETHYL ISOTHIOCYANATE
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C9H9NS
  1
1
IC50 = 2800 nM
 Bioactivity Info 
   TI
   LI
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CP0456778
 Compound Info 
1-(isothiocyanatomethyl)-4-methoxybenzene
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C9H9NOS
  1
1
IC50 = 3900 nM
 Bioactivity Info 
   TI
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CP0571415
 Compound Info 
4-(2-isothiocyanatoethyl)benzene-1,2-diol
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C9H9NO2S
  1
1
IC50 = 4000 nM
 Bioactivity Info 
   TI
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CP0429469
 Compound Info 
(6-Isothiocyanatohexyl)Benzene
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C13H17NS
  1
1
IC50 = 4200 nM
 Bioactivity Info 
   TI
   LI
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CP0556271
 Compound Info 
4-(1-isothiocyanatopropan-2-yl)phenol
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C10H11NOS
  1
1
IC50 = 4800 nM
 Bioactivity Info 
   TI
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CP0561039
 Compound Info 
5-(isothiocyanatomethyl)-1,3-benzodioxole
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C9H7NO2S
  1
1
IC50 = 5000 nM
 Bioactivity Info 
   TI
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CP0381962
 Compound Info 
6-isothiocyanatohexan-1-ol
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C7H13NOS
  1
1
IC50 = 5100 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0557211
 Compound Info 
1-(isothiocyanatomethyl)-3-(trifluoromethyl)benzene
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C9H6F3NS
  1
1
IC50 = 5900 nM
 Bioactivity Info 
   TI
   LI
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CP0396360
 Compound Info 
1-(2-isothiocyanatoethyl)-4-(trifluoromethyl)benzene
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C10H8F3NS
  1
1
IC50 = 7100 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0555779
 Compound Info 
3-(2-isothiocyanatoethyl)phenol
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C9H9NOS
  1
1
IC50 = 7800 nM
 Bioactivity Info 
   TI
   LI
   LO
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CP0396359
 Compound Info 
1-(2-isothiocyanatoethyl)-3-(trifluoromethyl)benzene
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C10H8F3NS
  1
1
IC50 = 10400 nM
 Bioactivity Info 
   TI
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CP0057544
 Compound Info 
CHEBI:85103
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C7H5NS
  1
1
IC50 = 11000 nM
 Bioactivity Info 
   TI
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CP0557209
 Compound Info 
methyl (5S)-3-(4-hydroxyphenyl)-4,5-dihydro-1,2-oxazole-5-carboxylate
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C11H11NO4
  1
1
IC50 = 11300 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0396358
 Compound Info 
1-isothiocyanatopropylbenzene
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C10H11NS
  1
1
IC50 = 21000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0396357
 Compound Info 
[isothiocyanato(phenyl)methyl]benzene
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C14H11NS
  1
1
IC50 > 100000 nM
 Bioactivity Info 
   TI
   LI
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CP0512564
 Compound Info 
CHEBI:60067
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C9H9NO
  1
1
IC50 > 100000 nM
 Bioactivity Info 
   TI
   LI
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   TS
CP0512565
 Compound Info 
4-[(4-hydroxyphenyl)-isothiocyanatomethyl]phenol
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C14H11NO2S
  1
1
IC50 > 100000 nM
 Bioactivity Info 
   TI
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CP0530833
 Compound Info 
BENZYL THIOCYANATE
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C8H7NS
  1
1
IC50 > 100000 nM
 Bioactivity Info 
   TI
   LI
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CP0557210
 Compound Info 
4-(1-isothiocyanatopropyl)phenol
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C10H11NOS
  1
1
IC50 > 100000 nM
 Bioactivity Info 
   TI
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Cell Line ID: CL000040 , THP-1
 Cell Line Info 
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0254279
 Compound Info 
2-(2-(3-(3-fluoro-4-hydroxyphenyl)-4,5-dihydroisoxazol-5-yl)ethyl)isoindoline-1,3-dione
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C19H15FN2O4
  2
1 IC50 = 800 nM
2 IC50 = 1500 nM
CP0136396
 Compound Info 
2-(3-(3-(3-fluoro-4-hydroxyphenyl)-4,5-dihydroisoxazol-5-yl)propyl)isoindoline-1,3-dione
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C20H17FN2O4
  1
1 IC50 = 7000 nM
CP0045136
 Compound Info 
methyl 2-[3-(4-hydroxyphenyl)-4,5-dihydro-1,2-oxazol-5-yl]acetate
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C12H13NO4
  7
1 IC50 = 7000 nM
2 IC50 = 11000 nM
3 IC50 = 25000 nM
4 IC50 = 65300 nM
5 IC50 = 79000 nM
6 IC50 > 100000 nM
7 Ki = 43500 nM
CP0136395
 Compound Info 
N-cyclohexyl-3-[3-[3-(3-fluoro-4-methoxyphenyl)-4,5-dihydro-1,2-oxazol-5-yl]-1,2,4-oxadiazol-5-yl]propanamide
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C21H25FN4O4
  1
1 IC50 = 9500 nM
CP0152831
 Compound Info 
4-fluoro-N-(2-(3-(3-fluoro-4-hydroxyphenyl)-4,5-dihydroisoxazol-5-yl)ethyl)benzamide
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C18H16F2N2O3
  2
1 IC50 = 10000 nM
2 IC50 = 20000 nM
CP0070450
 Compound Info 
2-fluoro-4-(5((5-(trifluoromethyl)-1,2,4-oxadiazole-3-yl)methyl)isoxazole-3-yl)phenol
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C13H9F4N3O3
  2
1 IC50 = 15000 nM
2 IC50 = 60000 nM
CP0070451
 Compound Info 
2,2,2-trifluoro-N-(2-(3-(3-fluoro-4-hydroxyphenyl)-4,5-dihydroisoxazol-5-yl)ethyl)acetamide
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C13H12F4N2O3
  2
1 IC50 = 15000 nM
2 IC50 > 100000 nM
CP0254282
 Compound Info 
N-[2-[3-(3-fluoro-4-hydroxyphenyl)-4,5-dihydro-1,2-oxazol-5-yl]ethyl]-2,2-dimethylpropanamide
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C16H21FN2O3
  2
1 IC50 = 15000 nM
2 IC50 = 22000 nM
CP0070452
 Compound Info 
1-(2-(3-(3-fluoro-4-hydroxyphenyl)-4,5-dihydroisoxazol-5-yl)ethyl)pyrrolidine-2,5-dione
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C15H15FN2O4
  2
1 IC50 = 20000 nM
2 IC50 = 50000 nM
CP0147396
 Compound Info 
N-[2-[3-(3-fluoro-4-hydroxyphenyl)-4,5-dihydro-1,2-oxazol-5-yl]ethyl]-3-methylbutanamide
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C16H21FN2O3
  2
1 IC50 = 20000 nM
2 IC50 = 22000 nM
CP0254276
 Compound Info 
5-(2,3-dimethoxyphenyl)-3-(3-(3-fluoro-4-methoxyphenyl)-4,5-dihydroisoxazol-5-yl)-1,2,4-oxadiazol
   Show/Hide
C20H18FN3O5
  1
1 IC50 = 32000 nM
CP0147393
 Compound Info 
2-((3-(3-fluoro-4-hydroxyphenyl)-4,5-dihydroisoxazol-5-yl)methyl)isoindoline-1,3-dione
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C18H13FN2O4
  1
1 IC50 = 36000 nM
CP0087418
 Compound Info 
3-((3-(3-fluoro-4-methoxyphenyl)-4,5-dihydroisoxazol-5-yl)methyl)-5-(trifluoromethyl)-1,2,4-oxadiazole
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C14H11F4N3O3
  1
1 IC50 = 55000 nM
CP0305782
 Compound Info 
3-(3-(3-(3-fluoro-4-methoxyphenyl)-4,5-dihydroisoxazole-5-yl)-1,2,4-oxadaizole-5-yl)propanoic acid
   Show/Hide
C15H14FN3O5
  2
1 IC50 = 60000 nM
2 IC50 > 100000 nM
CP0299356
 Compound Info 
3-(3-(3-fluoro-4-methoxyphenyl)-4,5-dihydroisoxazol-5-yl)-5-(trifluoromethyl)-1,2,4-oxadiazole
   Show/Hide
C13H9F4N3O3
  1
1 IC50 = 95000 nM
CP0254281
 Compound Info 
3-(3-(3-(3-fluoro-4-hydroxyphenyl)-4,5-dihydroisoxazol-5-yl)-1,2,4-oxadiazol-5-yl)benzene-1-2-diol
   Show/Hide
C17H12FN3O5
  1
1 IC50 > 100000 nM
CP0299357
 Compound Info 
N-cyclohexyl-3-[3-[3-(3-fluoro-4-hydroxyphenyl)-4,5-dihydro-1,2-oxazol-5-yl]-1,2,4-oxadiazol-5-yl]propanamide
   Show/Hide
C20H23FN4O4
  1
1 IC50 > 100000 nM
CP0302685
 Compound Info 
2-fluoro-4-(5-(5-(trifluoromethyl)-1,2,4-oxadiazole-3-yl)-4,5-dihydroisoxazole-3-yl)phenol
   Show/Hide
C12H7F4N3O3
  1
1 IC50 > 100000 nM
CP0302740
 Compound Info 
methyl 3-[3-[3-(3-fluoro-4-hydroxyphenyl)-4,5-dihydro-1,2-oxazol-5-yl]-1,2,4-oxadiazol-5-yl]propanoate
   Show/Hide
C15H14FN3O5
  1
1 IC50 > 100000 nM
CP0357273
 Compound Info 
2-fluoro-4-(5-(5-methyl-1,2,4-oxadiazol-3-yl)-4,5-dihydroisoxazol-3-yl)phenol
   Show/Hide
C12H10FN3O3
  1
1 IC50 > 100000 nM
Clinical Information about the Protein
Target 1 ( Macrophage migration inhibitory factor (MIF) )
Target Type Clinical trial Target
Disease 2 Target-related Diseases  2
1 Thrombocytopenia [ICD-11: 3B64]
2 Autoimmune disease [ICD-11: 4A40-4A45]
Approved Drug(s) 1 Approved Drug  1
1 3,4-Dihydroxycinnamic Acid
 Drug Info 
Phase 4
Thrombocytopenia
Investigative Drug(s) 1 Investigative Drug  1
1 NAPQI
 Drug Info 
Investigative
Autoimmune disease
Target 2 ( MIF messenger RNA (MIF mRNA) )
Target Type Literature-reported Target
Disease 1 Target-related Disease  1
1 Solid tumour/cancer [ICD-11: 2A00-2F9Z]
Investigative Drug(s) 1 Investigative Drug  1
1 4-iodo-6-phenylpyrimidine
 Drug Info 
Investigative
Solid tumour/cancer