General Information of the Protein
Protein ID
PT00018
Protein Name
Neuraminidase
Sequence
MNPNQKIITIGSICMVVGIISLILQIGNIISIWISHSIQTGNQNHTGICNQGSITYKVVAGQDSTSVILTGNSSLCPIRGWAIHSKDNGIRIGSKGDVFVIREPFISCSHLECRTFFLTQGALLNDKHSRGTFKDRSPYRALMSCPVGEAPSPYNSRFESVAWSASACHDGMGWLTIGISGPDDGAVAVLKYNGIITETIKSWRKNILRTQESECTCVNGSCFTIMTDGPSDGLASYKIFKIEKGKVTKSIELNAPNSHYEECSCYPDTGKVMCVCRDNWHGSNRPWVSFDQNLDYKIGYICSGVFGDNPRPKDGTGSCGPVSADGANGVKGFSYKYGNGVWIGRTKSDSSRHGFEMIWDPNGWTETDSRFSMRQDVVAMTDRSGYSGSFVQHPELTGLDCMRPCFWVELIRGLPEENAIWTSGSIISFCGVNSDTVDWSWPDGAELPFTIDK
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Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1), Influenza A virus (strain A/WS/1933 H1N1)
Protein Classification
Enzyme
>
Hydrolase
Function
Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.
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Uniprot ID
B4URF0
Subcellular Location
Membrane
Virion membrane
Host apical cell membrane
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000025 , HEK-293T
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0071620
 Compound Info 
CHEBI:66265
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C18H18O5
  3
1
IC50 = 2550 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
IC50 = 10000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
3
Ki = 34140 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0105780
 Compound Info 
CHEBI:70655
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C18H18O5
  3
1
IC50 = 3310 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
IC50 = 8150 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
3
Ki = 24900 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0228529
 Compound Info 
CHEBI:70658
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C18H18O4
  3
1
IC50 = 5030 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
IC50 = 35230 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
3
Ki = 41370 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0036725
 Compound Info 
Oseltamivir
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C16H28N2O4
  2
1
IC50 = 12290 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
IC50 = 51540 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0071621
 Compound Info 
CHEBI:70659
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C17H16O4
  3
1
IC50 = 26020 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
2
IC50 = 93770 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
3
Ki = 12000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Cell Line ID: CL000006 , HEK293
 Cell Line Info 
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0036726
 Compound Info 
(2R,3R,4S)-4-carbamimidamido-3-acetamido-2-[(1R,2R)-1,2,3-trihydroxypropyl]-3,4-dihydro-2H-pyran-6-carboxylic acid
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C12H20N4O7
  102
1 IC50 = 0.03 nM
2 IC50 = 0.04 nM
3 IC50 = 0.05 nM
4 IC50 = 0.2 nM
5 IC50 = 0.29 nM
6 IC50 = 0.45 nM
7 IC50 = 0.47 nM
8 IC50 = 0.49 nM
9 IC50 = 0.58 nM
10 IC50 = 0.66 nM
11 IC50 = 0.7 nM
12 IC50 = 0.751 nM
13 IC50 = 0.8 nM
14 IC50 = 0.88 nM
15 IC50 = 1 nM
16 IC50 = 1.1 nM
17 IC50 = 1.14 nM
18 IC50 = 1.17 nM
19 IC50 = 1.19 nM
20 IC50 = 1.38 nM
21 IC50 = 1.4 nM
22 IC50 = 1.5 nM
23 IC50 = 1.6 nM
24 IC50 = 1.63 nM
25 IC50 = 1.66 nM
26 IC50 = 1.68 nM
27 IC50 = 1.8 nM
28 IC50 = 1.83 nM
29 IC50 = 1.9 nM
30 IC50 = 2 nM
31 IC50 = 2.05 nM
32 IC50 = 2.17 nM
33 IC50 = 2.2 nM
34 IC50 = 2.33 nM
35 IC50 = 2.4 nM
36 IC50 = 2.42 nM
37 IC50 = 2.5 nM
38 IC50 = 2.55 nM
39 IC50 = 2.7 nM
40 IC50 = 2.99 nM
41 IC50 = 3.05 nM
42 IC50 = 3.31 nM
43 IC50 = 3.4 nM
44 IC50 = 3.42 nM
45 IC50 = 3.51 nM
46 IC50 = 3.62 nM
47 IC50 = 3.65 nM
48 IC50 = 3.66 nM
49 IC50 = 3.97 nM
50 IC50 = 4 nM
51 IC50 = 4.32 nM
52 IC50 = 4.82 nM
53 IC50 = 4.84 nM
54 IC50 = 5 nM
55 IC50 = 5.11 nM
56 IC50 = 5.44 nM
57 IC50 = 5.53 nM
58 IC50 = 5.58 nM
59 IC50 = 5.8 nM
60 IC50 = 5.86 nM
61 IC50 = 6 nM
62 IC50 = 6.04 nM
63 IC50 = 6.45 nM
64 IC50 = 6.82 nM
65 IC50 = 7.16 nM
66 IC50 = 7.33 nM
67 IC50 = 7.35 nM
68 IC50 = 7.39 nM
69 IC50 = 7.58 nM
70 IC50 = 7.71 nM
71 IC50 = 7.8 nM
72 IC50 = 7.82 nM
73 IC50 = 8.21 nM
74 IC50 = 8.29 nM
75 IC50 = 8.57 nM
76 IC50 = 8.74 nM
77 IC50 = 9.08 nM
78 IC50 = 9.15 nM
79 IC50 = 9.3 nM
80 IC50 = 9.81 nM
81 IC50 = 10 nM
82 IC50 = 10.7 nM
83 IC50 = 11 nM
84 IC50 = 11.2 nM
85 IC50 = 11.5 nM
86 IC50 = 11.51 nM
87 IC50 = 12 nM
88 IC50 = 13.01 nM
89 IC50 = 13.5 nM
90 IC50 = 13.8 nM
91 IC50 = 14.2 nM
92 IC50 = 17.61 nM
93 IC50 = 22.8 nM
94 IC50 = 33.55 nM
95 IC50 = 500 nM
96 IC50 = 678.4 nM
97 IC50 = 815.9 nM
98 IC50 = 1659 nM
99 Ki = 0.06 nM
100 Ki = 0.16 nM
101 Ki = 0.2 nM
102 Ki = 0.3 nM
CP0036725
 Compound Info 
Oseltamivir
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C16H28N2O4
  73
1 IC50 = 0.06 nM
2 IC50 = 0.16 nM
3 IC50 = 0.19 nM
4 IC50 = 0.2 nM
5 IC50 = 0.3 nM
6 IC50 = 0.5 nM
7 IC50 = 0.658 nM
8 IC50 = 0.706 nM
9 IC50 = 0.711 nM
10 IC50 = 0.785 nM
11 IC50 = 0.87 nM
12 IC50 = 0.885 nM
13 IC50 = 0.925 nM
14 IC50 = 0.981 nM
15 IC50 = 1 nM
16 IC50 = 1.08 nM
17 IC50 = 1.09 nM
18 IC50 = 1.12 nM
19 IC50 = 1.18 nM
20 IC50 = 1.2 nM
21 IC50 = 1.25 nM
22 IC50 = 1.26 nM
23 IC50 = 1.33 nM
24 IC50 = 1.34 nM
25 IC50 = 1.43 nM
26 IC50 = 1.5 nM
27 IC50 = 1.54 nM
28 IC50 = 1.56 nM
29 IC50 = 1.58 nM
30 IC50 = 1.59 nM
31 IC50 = 1.6 nM
32 IC50 = 1.7 nM
33 IC50 = 1.71 nM
34 IC50 = 1.78 nM
35 IC50 = 1.86 nM
36 IC50 = 1.9 nM
37 IC50 = 1.99 nM
38 IC50 = 2.12 nM
39 IC50 = 2.17 nM
40 IC50 = 2.28 nM
41 IC50 = 2.39 nM
42 IC50 = 2.5 nM
43 IC50 = 2.56 nM
44 IC50 = 2.8 nM
45 IC50 = 2.81 nM
46 IC50 = 2.94 nM
47 IC50 = 3.44 nM
48 IC50 = 3.65 nM
49 IC50 = 4.95 nM
50 IC50 = 8 nM
51 IC50 = 10 nM
52 IC50 = 14.8 nM
53 IC50 = 15.812554 nM
54 IC50 = 16 nM
55 IC50 = 20 nM
56 IC50 = 37.2 nM
57 IC50 = 50 nM
58 IC50 = 67.507442 nM
59 IC50 = 80 nM
60 IC50 = 127.204635 nM
61 IC50 = 136.9 nM
62 IC50 = 140 nM
63 IC50 = 150 nM
64 IC50 = 210 nM
65 IC50 = 755 nM
66 IC50 = 4190 nM
67 IC50 = 10340 nM
68 IC50 = 10400 nM
69 IC50 = 16420.72917 nM
70 IC50 = 79940 nM
71 IC50 = 95220 nM
72 Ki = 0.1 nM
73 Ki = 0.9 nM
CP0046477
 Compound Info 
(3R,4R,5S)-5-amino-4-acetamido-3-(pentan-3-yloxy)cyclohex-1-ene-1-carboxylic acid
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C14H24N2O4
  39
1 IC50 = 0.1 nM
2 IC50 = 0.12 nM
3 IC50 = 0.14 nM
4 IC50 = 0.15 nM
5 IC50 = 0.17 nM
6 IC50 = 0.18 nM
7 IC50 = 0.44 nM
8 IC50 = 1 nM
9 IC50 = 2.1 nM
10 IC50 = 2.8 nM
11 IC50 = 2.9 nM
12 IC50 = 3.1 nM
13 IC50 = 3.2 nM
14 IC50 = 3.3 nM
15 IC50 = 4.17 nM
16 IC50 = 4.45 nM
17 IC50 = 5.4 nM
18 IC50 = 7.14 nM
19 IC50 = 7.77 nM
20 IC50 = 8.42 nM
21 IC50 = 14.5 nM
22 IC50 = 15 nM
23 IC50 = 15.72 nM
24 IC50 = 21 nM
25 IC50 = 23 nM
26 IC50 = 33.56 nM
27 IC50 = 36.1 nM
28 IC50 = 53.2 nM
29 IC50 = 210 nM
30 IC50 = 217 nM
31 IC50 = 235 nM
32 IC50 = 250 nM
33 IC50 = 330 nM
34 IC50 = 370 nM
35 IC50 = 817.43 nM
36 IC50 = 1160 nM
37 IC50 = 3840 nM
38 IC50 = 5050 nM
39 IC50 > 100 nM
CP0078933
 Compound Info 
(-)-(1S,2S,3R,4R)-3-[(1S)-1-(Acetylamino)-2-ethylbutyl]-4-{[amino(imino)methyl]amino}-2-hydroxycyclopentanecarboxylic Acid
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C15H28N4O4
  3
1 IC50 = 0.1 nM
2 IC50 = 23.1 nM
3 IC50 = 10000 nM
CP0257627
 Compound Info 
(1S,2S,3S,4R)-3-[(1S)-1-acetamido-2-ethylbutyl]-4-(diaminomethylideneamino)-2-[3-[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxypropylcarbamoyloxy]cyclopentane-1-carboxylic acid
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C28H41N5O9
  1
1 IC50 = 10.5 nM
CP0178991
 Compound Info 
(1S,2S,3S,4R)-3-[(1S)-1-acetamido-2-ethylbutyl]-4-(diaminomethylideneamino)-2-[3-[[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]amino]propylcarbamoyloxy]cyclopentane-1-carboxylic acid
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C28H42N6O8
  1
1 IC50 = 10.9 nM
CP0105780
 Compound Info 
CHEBI:70655
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C18H18O5
  4
1 IC50 = 18570 nM
2 IC50 = 18600 nM
3 IC50 = 20400 nM
4 IC50 = 20450 nM
CP0128465
 Compound Info 
(E)-3-(4-Hydroxy-2-methoxy-phenyl)-1-(4-hydroxy-phenyl)-propenone
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C16H14O4
  3
1 IC50 = 21089.24079 nM
2 IC50 = 21459.22747 nM
3 IC50 = 30449.9038 nM
CP0228529
 Compound Info 
CHEBI:70658
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C18H18O4
  2
1 IC50 = 21740 nM
2 IC50 = 32480 nM
CP0071620
 Compound Info 
CHEBI:66265
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C18H18O5
  3
1 IC50 = 23100 nM
2 IC50 = 23120 nM
3 IC50 = 28120 nM
CP0040451
 Compound Info 
(E)-1-(2,4-Dihydroxy-phenyl)-3-(4-hydroxy-phenyl)-propenone
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C15H12O4
  3
1 IC50 = 32818.23148 nM
2 IC50 = 37813.15851 nM
3 IC50 = 41208.14798 nM
CP0041330
 Compound Info 
Kaempferol 3-O-&#946;-D-glucoside (6)
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C21H20O11
  2
1 IC50 = 38400 nM
2 Ki = 33000 nM
CP0070522
 Compound Info 
(E)-3-(4-hydroxy-2-methoxy-5-(2-methylbut-3-en-2-yl)phenyl)-1-(4-hydroxyphenyl)prop-2-en-1-one
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C21H22O4
  3
1 IC50 = 53132.38771 nM
2 IC50 = 53871.15839 nM
3 IC50 = 56412.52955 nM
CP0071621
 Compound Info 
CHEBI:70659
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C17H16O4
  2
1 IC50 = 66480 nM
2 IC50 = 85740 nM
Similar Protein(s) and Bioactivity Statistics
50% Identity
Protein ID Protein Name Protein Organism
PT01603 Neuraminidase Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
 Protein Info 
PT02681 Neuraminidase Influenza A virus (strain A/Wilson-Smith/1933 H1N1), Influenza A virus (strain A/WS/1933 H1N1)
 Protein Info