Brief Information of the Compound and Protein
Compound Name
 Compound Info 
11-[(4-methylpiperazin-1-yl)acetyl]-5,11-dihydro-6H-pyrido[2,3-b][1,4]benzodiazepin-6-one
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 PubChem ID
CID: 4848
SID: 14852009
Canonical SMILES
CN1CCN(CC(=O)N2c3ccccc3C(=O)Nc3cccnc23)CC1
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Compound Formula C19H21N5O2
Compound Structure
Protein Name
 Protein Info 
Muscarinic acetylcholine receptor M1
Protein Classification
Membrane receptor
>
Family A G protein-coupled receptor
>
Small molecule receptor (family A GPCR)
>
Monoamine receptor
>
Acetylcholine receptor
Uniprot ID
P11229
Gene Name
CHRM1
Table of Molecular Bioactivities Related to the Compound and Protein
Cell-based Assay
Cell Line ID Cell Line Name Cell Line Organism
CL000011 CHO
 Cell Line Info 
Cricetulus griseus (Chinese hamster)  9
1
IC50 = 21 nM
   TI
   LI
   LO
   TS
2
IC50 = 27 nM
   TI
   LI
   LO
   TS
3
IC50 = 28.2 nM
   TI
   LI
   LO
   TS
4
IC50 = 119 nM
   TI
   LI
   LO
   TS
5
Ki = 15 nM
   TI
   LI
   LO
   TS
6
Ki = 16 nM
   TI
   LI
   LO
   TS
7
Ki = 20 nM
   TI
   LI
   LO
   TS
8
Ki = 21.38 nM
   TI
   LI
   LO
   TS
9
Ki = 24 nM
   TI
   LI
   LO
   TS
CL000026 CHO-K1
 Cell Line Info 
Cricetulus griseus (Chinese hamster)  1
1
Ki = 2.818 nM
   TI
   LI
   LO
   TS
CL000025 HEK-293T
 Cell Line Info 
Homo sapiens (Human)  3
1
IC50 = 1.479 nM
   TI
   LI
   LO
   TS
2
IC50 = 20.42 nM
   TI
   LI
   LO
   TS
3
IC50 = 48.98 nM
   TI
   LI
   LO
   TS
CL000006 HEK293
 Cell Line Info 
Homo sapiens (Human)  1
1
Ki = 23.5 nM
   TI
   LI
   LO
   TS
Biochemical Assays
1 IC50 = 12 nM
2 IC50 = 19 nM
3 IC50 = 22 nM
4 IC50 = 27 nM
5 IC50 = 29 nM
6 Kd = 20 nM
7 Ki = 9.1 nM
8 Ki = 10 nM
9 Ki = 15 nM
10 Ki = 23.5 nM
11 Ki = 25 nM