Brief Information of the Compound and Protein
Compound Name
 Compound Info 
(oxotremorine)1-(4-Pyrrolidin-1-yl-but-2-ynyl)-pyrrolidin-2-one
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 PubChem ID
CID: 4630
SID: 15121093
Canonical SMILES
O=C1CCCN1CC#CCN1CCCC1
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Compound Formula C12H18N2O
Compound Structure
Protein Name
 Protein Info 
Muscarinic acetylcholine receptor M4
Protein Classification
Membrane receptor
>
Family A G protein-coupled receptor
>
Small molecule receptor (family A GPCR)
>
Monoamine receptor
>
Acetylcholine receptor
Uniprot ID
P08173
Gene Name
CHRM4
Table of Molecular Bioactivities Related to the Compound and Protein
Cell-based Assay
Cell Line ID Cell Line Name Cell Line Organism
CL000011 CHO
 Cell Line Info 
Cricetulus griseus (Chinese hamster)  3
1
IC50 = 5000 nM
   TI
   LI
   LO
   TS
2
Ki = 1.5 nM
   TI
   LI
   LO
   TS
3
Ki = 160 nM
   TI
   LI
   LO
   TS
CL000006 HEK293
 Cell Line Info 
Homo sapiens (Human)  2
1
EC50 = 9 nM
   TI
   LI
   LO
   TS
2
EC50 = 14 nM
   TI
   LI
   LO
   TS
Biochemical Assays
1 EC50 = 33 nM