General Information of the Protein
Protein ID
PT06173
Protein Name
U5 small nuclear ribonucleoprotein 200 kDa helicase
Secondarily
Protein Name
Activating signal cointegrator 1 complex subunit 3-like 1
BRR2 homolog
U5 snRNP-specific 200 kDa protein
Gene Name
SNRNP200
Secondarily
Gene Name
ASCC3L1
BRR2
HELIC2
KIAA0788
Sequence
MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTKPQMQEERRAKRRKRDEDRHDINKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFIQAALGDQPRDILCGAADEVLAVLKNEKLRDKERRKEIDLLLGQTDDTRYHVLVNLGKKITDYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTLSANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKMEADPELSKFLYQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKGGERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD
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Organism
Homo sapiens, Human
Protein Classification
Enzyme
>
Hydrolase
Function
Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome (PubMed:35241646). Plays a role in pre-mRNA splicing as a core component of precatalytic, catalytic and postcatalytic spliceosomal complexes (PubMed:28502770, PubMed:28781166, PubMed:29361316, PubMed:30315277, PubMed:29360106, PubMed:29301961, PubMed:30728453, PubMed:30705154). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome.
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Uniprot ID
Primary ID:
O75643

Secondarily ID:
O94884
Q6NZY0
Q6PX59
Q8NBE6
Q96IF2
Q9H7S0
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Ensembl ID
ENSG00000144028
HGNC ID
HGNC:30859
Subcellular Location
Nucleus
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000013 , Sf9
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0558808
 Compound Info 
methyl (3S,3'R,5'S)-1'-(1-benzylimidazole-4-carbonyl)-5-fluoro-5'-(2-methylpropyl)-2-oxospiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C28H29FN4O4
  1
1
IC50 = 44 nM
 Bioactivity Info 
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CP0412858
 Compound Info 
6-benzyl-3-[3-(1,3-thiazol-5-ylmethoxy)phenyl]-1,4-dihydropyrido[4,3-d]pyrimidine-2,7-dione
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C24H20N4O3S
  2
1
IC50 = 79 nM
 Bioactivity Info 
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2
IC50 = 1300 nM
 Bioactivity Info 
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CP0558807
 Compound Info 
methyl (3S,3'R,5'S)-5-fluoro-1'-(1-methylimidazole-4-carbonyl)-5'-(2-methylpropyl)-2-oxospiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C22H25FN4O4
  1
1
IC50 = 110 nM
 Bioactivity Info 
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CP0468071
 Compound Info 
6-benzyl-3-[3-(pyridin-3-ylmethoxy)phenyl]-1,4-dihydropyrido[4,3-d]pyrimidine-2,7-dione
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C26H22N4O3
  1
1
IC50 = 180 nM
 Bioactivity Info 
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CP0462041
 Compound Info 
(3S,3'R,5'S)-1'-(1-benzylimidazole-4-carbonyl)-5-fluoro-3'-methyl-5'-(2-methylpropyl)spiro[1H-indole-3,2'-pyrrolidine]-2-one
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C27H29FN4O2
  1
1
IC50 = 350 nM
 Bioactivity Info 
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CP0539047
 Compound Info 
(3S,3'R,5'S)-3'-methyl-5'-(2-methylpropyl)-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-2-one
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C22H25N3O2
  1
1
IC50 = 360 nM
 Bioactivity Info 
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CP0456407
 Compound Info 
methyl (3S,3'R,5'S)-5-fluoro-1'-(1H-imidazole-5-carbonyl)-5'-(2-methylpropyl)-2-oxospiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C21H23FN4O4
  1
1
IC50 = 390 nM
 Bioactivity Info 
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CP0447207
 Compound Info 
(3S,3'R,5'S)-5-fluoro-3'-methyl-1'-(1-methylimidazole-4-carbonyl)-5'-(2-methylpropyl)spiro[1H-indole-3,2'-pyrrolidine]-2-one
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C21H25FN4O2
  1
1
IC50 = 480 nM
 Bioactivity Info 
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CP0468070
 Compound Info 
6-benzyl-3-(3-phenylmethoxyphenyl)-1,4-dihydropyrido[4,3-d]pyrimidine-2,7-dione
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C27H23N3O3
  1
1
IC50 = 790 nM
 Bioactivity Info 
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CP0558964
 Compound Info 
methyl (3S,3'R,5'S)-5-fluoro-5'-(2-methylpropyl)-2-oxo-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C23H24FN3O4
  1
1
IC50 = 910 nM
 Bioactivity Info 
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CP0558806
 Compound Info 
(3S,3'R,5'S)-3'-(fluoromethyl)-5'-(2-methylpropyl)-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-2-one
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C22H24FN3O2
  1
1
IC50 = 1300 nM
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CP0493366
 Compound Info 
6-benzyl-3-[3-(pyridin-2-ylmethoxy)phenyl]-1,4-dihydropyrido[4,3-d]pyrimidine-2,7-dione
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C26H22N4O3
  1
1
IC50 = 1400 nM
 Bioactivity Info 
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CP0565179
 Compound Info 
6-benzyl-3-[2-(3-fluoropyridin-2-yl)-3,4-dihydro-2H-chromen-7-yl]-1,4-dihydropyrido[4,3-d]pyrimidine-2,7-dione
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C28H23FN4O3
  1
1
IC50 = 1700 nM
 Bioactivity Info 
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CP0481061
 Compound Info 
methyl (3S,3'R,5'S)-5-fluoro-1'-(1-methylimidazole-2-carbonyl)-5'-(2-methylpropyl)-2-oxospiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C22H25FN4O4
  1
1
IC50 = 1700 nM
 Bioactivity Info 
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CP0558232
 Compound Info 
(3S,3'R,5'S)-3'-(chloromethyl)-5'-(2-methylpropyl)-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-2-one
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C22H24ClN3O2
  1
1
IC50 = 2800 nM
 Bioactivity Info 
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CP0476122
 Compound Info 
methyl (3S,3'R,5'S)-4-fluoro-5'-(2-methylpropyl)-2-oxo-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C23H24FN3O4
  1
1
IC50 = 3400 nM
 Bioactivity Info 
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CP0412859
 Compound Info 
3-[5-[(Z)-(5-imino-7-oxo-2-propyl-[1,3,4]thiadiazolo[3,2-a]pyrimidin-6-ylidene)methyl]furan-2-yl]benzoic acid
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C20H16N4O4S
  1
1
IC50 = 4200 nM
 Bioactivity Info 
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CP0403312
 Compound Info 
methyl (3S,3'R,5'S)-7-fluoro-5'-(2-methylpropyl)-2-oxo-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C23H24FN3O4
  1
1
IC50 = 4300 nM
 Bioactivity Info 
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CP0436680
 Compound Info 
methyl (3S,3'R,5'S)-5-fluoro-5'-(2-methylpropyl)-1'-(1-methylpyrazole-3-carbonyl)-2-oxospiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C22H25FN4O4
  1
1
IC50 = 4600 nM
 Bioactivity Info 
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CP0403361
 Compound Info 
6-benzyl-3-[2-(3-fluoropyridin-2-yl)-6-methyl-3,4-dihydro-2H-chromen-7-yl]-1,4-dihydropyrido[4,3-d]pyrimidine-2,7-dione
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C29H25FN4O3
  1
1
IC50 = 5300 nM
 Bioactivity Info 
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CP0469863
 Compound Info 
3-[5-[(Z)-(2-cyclohexyl-5-imino-7-oxo-[1,3,4]thiadiazolo[3,2-a]pyrimidin-6-ylidene)methyl]furan-2-yl]benzoic acid
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C23H20N4O4S
  1
1
IC50 = 5300 nM
 Bioactivity Info 
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CP0436679
 Compound Info 
methyl (3S,3'R,5'S)-5-chloro-5'-(2-methylpropyl)-2-oxo-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C23H24ClN3O4
  1
1
IC50 = 8000 nM
 Bioactivity Info 
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CP0403311
 Compound Info 
(3S,3'R,5'S)-3'-(hydroxymethyl)-5'-(2-methylpropyl)-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-2-one
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C22H25N3O3
  1
1
IC50 = 12000 nM
 Bioactivity Info 
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CP0403360
 Compound Info 
3-[2-(3-fluoropyridin-2-yl)-6-methyl-3,4-dihydro-2H-chromen-7-yl]-6-(2,2,2-trifluoroethyl)-1,4-dihydropyrido[4,3-d]pyrimidine-2,7-dione
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C24H20F4N4O3
  1
1
IC50 = 36000 nM
 Bioactivity Info 
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CP0407822
 Compound Info 
6-benzyl-3-(2-phenylmethoxyphenyl)-1,4-dihydropyrido[4,3-d]pyrimidine-2,7-dione
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C27H23N3O3
  1
1
IC50 = 58000 nM
 Bioactivity Info 
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CP0463438
 Compound Info 
(3R,3'S,5'R)-5-fluoro-3'-methyl-1'-(1-methylimidazole-4-carbonyl)-5'-(2-methylpropyl)spiro[1H-indole-3,2'-pyrrolidine]-2-one
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C21H25FN4O2
  1
1
IC50 = 84000 nM
 Bioactivity Info 
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CP0476123
 Compound Info 
methyl (3S,3'R,5'S)-6-fluoro-5'-(2-methylpropyl)-2-oxo-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C23H24FN3O4
  1
1
IC50 = 88000 nM
 Bioactivity Info 
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CP0468069
 Compound Info 
3-[2-(3-fluoropyridin-2-yl)-6-methyl-3,4-dihydro-2H-chromen-7-yl]-6-methyl-1,4-dihydropyrido[4,3-d]pyrimidine-2,7-dione
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C23H21FN4O3
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0487763
 Compound Info 
6-benzyl-3-[2-(3-fluoropyridin-2-yl)-6-methyl-3,4-dihydro-2H-chromen-7-yl]-1-methyl-4H-pyrido[4,3-d]pyrimidine-2,7-dione
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C30H27FN4O3
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0403310
 Compound Info 
methyl (3R,3'S,5'R)-5'-(2-methylpropyl)-2-oxo-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C23H25N3O4
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0476120
 Compound Info 
methyl (3S,3'R,5'S)-5'-(2-methylpropyl)-2-oxo-1'-(2-pyridin-2-ylacetyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C24H27N3O4
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0476121
 Compound Info 
methyl (3S,3'R,5'S)-5'-methyl-2-oxo-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C20H19N3O4
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0477034
 Compound Info 
(3R,3'S,5'R)-1'-(1-benzylimidazole-4-carbonyl)-5-fluoro-3'-methyl-5'-(2-methylpropyl)spiro[1H-indole-3,2'-pyrrolidine]-2-one
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C27H29FN4O2
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0481059
 Compound Info 
methyl (3S,3'R,5'S)-5-fluoro-5'-(2-methylpropyl)-2-oxo-1'-(pyridine-3-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C23H24FN3O4
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0481060
 Compound Info 
methyl (3S,3'R,5'S)-5-fluoro-5'-(2-methylpropyl)-2-oxo-1'-(pyridine-4-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C23H24FN3O4
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0488964
 Compound Info 
(3S,3'R,5'S)-5'-(2-methylpropyl)-2-oxo-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxamide
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C22H24N4O3
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0488965
 Compound Info 
methyl (3S,3'R,5'S)-1'-benzoyl-5-fluoro-5'-(2-methylpropyl)-2-oxospiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C24H25FN2O4
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0513727
 Compound Info 
methyl (3S,3'S,5'S)-5'-(2-methylpropyl)-2-oxo-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C23H25N3O4
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0513728
 Compound Info 
methyl (3S,3'R,5'R)-5'-benzyl-2-oxo-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylate
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C26H23N3O4
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0558805
 Compound Info 
(3S,3'R,5'S)-5'-(2-methylpropyl)-2-oxo-1'-(pyridine-2-carbonyl)spiro[1H-indole-3,2'-pyrrolidine]-3'-carboxylic acid;hydrochloride
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C22H24ClN3O4
  1
1
IC50 > 100000 nM
 Bioactivity Info 
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CP0574422
 Compound Info 
methyl (3S,3'R,5'S)-1-methyl-5'-(2-methylpropyl)-2-oxo-1'-(pyridine-2-carbonyl)spiro[indole-3,2'-pyrrolidine]-3'-carboxylate
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C24H27N3O4
  1
1
IC50 > 100000 nM
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