General Information of the Protein
Protein ID
PT01911
Protein Name
Nitric oxide synthase 1
Secondarily
Protein Name
Constitutive NOS
NC-NOS
NOS type I
Neuronal NOS
Nitric oxide synthase
brain
Peptidyl-cysteine S-nitrosylase NOS1
Gene Name
NOS1
Secondarily
Gene Name
Bnos
Sequence
MEENTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPSASKDQSLAVDRVTGLGNGPQHAQGHGQGAGSVSQANGVAIDPTMKSTKANLQDIGEHDELLKEIEPVLSILNSGSKATNRGGPAKAEMKDTGIQVDRDLDGKSHKAPPLGGDNDRVFNDLWGKDNVPVILNNPYSEKEQSPTSGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEHICMGSIMLPSQHTRKPEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKAPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKPNNSLISNDRSWKRNKFRLTYVAEAPDLTQGLSNVHKKRVSAARLLSRQNLQSPKFSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDADEVFSS
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Organism
Rattus norvegicus, Rat
Protein Classification
Enzyme
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.
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Uniprot ID
Primary ID:
P29476

Secondarily ID:
P70594
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Ensembl ID
ENSG00000089250
Subcellular Location
Cell membrane
Sarcolemma
Cell projection
Dendritic spine
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000025 , HEK-293T
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0067773
 Compound Info 
(2S)-2-amino-5-{[(E)-amino(nitroimino)methyl]amino}pentanoic acid
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C6H13N5O4
  1
1
IC50 = 5000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0454725
 Compound Info 
6-[[(3S,4S)-4-[2-[(2-fluorophenyl)methylamino]ethoxy]pyrrolidin-3-yl]methyl]-4-methylpyridin-2-amine
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C20H27FN4O
  1
1
IC50 = 11000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0107463
 Compound Info 
(+/-)-cis-6-((4-(2-(3-Fluorophenethylamino)-ethoxy)-pyrrolidin-3-yl)methyl)-4-methyl-pyridin-2-amine
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C21H29FN4O
  1
1
IC50 = 13000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0088832
 Compound Info 
(+/-)-cis-N1-((3S,4S)-4-((6-amino-4-methylpyridin-2-yl)methyl)pyrrolidin-3-yl)-N2-(3-fluorophenethyl)ethane-1,2-diamine
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C21H30FN5
  1
1
IC50 = 19000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0418867
 Compound Info 
6-[[(3S,4S)-4-[2-[(2-methoxyphenyl)methylamino]ethoxy]pyrrolidin-3-yl]methyl]-4-methylpyridin-2-amine
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C21H30N4O2
  1
1
IC50 = 22000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0919329
 Compound Info 
2-amino-4,6-dimethylpyridine hydrochloride
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C7H11ClN2
  1
1
IC50 = 25000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0847140
 Compound Info 
(+/-)-N1-(4-((6-amino-4-methylpyridin-2-yl)methyl)pyrrolidin-3-yl)-N2-(4-chlorobenzyl)ethane-1,2-diamine
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C20H28ClN5
  1
1
IC50 = 42000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0376908
 Compound Info 
2-[[2-[(3S,4S)-4-[(6-amino-4-methylpyridin-2-yl)methyl]pyrrolidin-3-yl]oxyethylamino]methyl]phenol
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C20H28N4O2
  1
1
IC50 = 43000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
CP0418868
 Compound Info 
6-[[(3S,4S)-4-[2-(furan-2-ylmethylamino)ethoxy]pyrrolidin-3-yl]methyl]-4-methylpyridin-2-amine
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C18H26N4O2
  1
1
IC50 = 100000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Cell Line ID: CL000013 , Sf9
 Cell Line Info 
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0067773
 Compound Info 
(2S)-2-amino-5-{[(E)-amino(nitroimino)methyl]amino}pentanoic acid
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C6H13N5O4
  3
1 IC50 = 600 nM
2 IC50 = 6000 nM
3 Ki = 4550 nM
CP0088832
 Compound Info 
(+/-)-cis-N1-((3S,4S)-4-((6-amino-4-methylpyridin-2-yl)methyl)pyrrolidin-3-yl)-N2-(3-fluorophenethyl)ethane-1,2-diamine
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C21H30FN5
  2
1 Ki = 5 nM
2 Ki = 4060 nM
CP0107463
 Compound Info 
(+/-)-cis-6-((4-(2-(3-Fluorophenethylamino)-ethoxy)-pyrrolidin-3-yl)methyl)-4-methyl-pyridin-2-amine
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C21H29FN4O
  2
1 Ki = 7 nM
2 Ki = 9500 nM