General Information of the Protein
Protein ID
PT01347
Protein Name
LIM domain kinase 2
Gene Name
LIMK2
Sequence
MSALAGEDVWRCPGCGDHIAPSQIWYRTVNETWHGSCFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP
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Organism
Homo sapiens, Human
Protein Classification
Enzyme
>
Kinase
>
Protein Kinase
>
TKL protein kinase group
>
TKL protein kinase LISK family
>
TKL protein kinase LIMK subfamily
Function
Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed:10436159, PubMed:11018042). Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, PubMed:11018042). Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed:22328514). Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed:8537403). Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865).
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Uniprot ID
Primary ID:
P53671

Secondarily ID:
A8K6H5
Q7KZ80
Q7L3H5
Q96E10
Q99464
Q9UFU0
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Ensembl ID
ENSG00000182541
HGNC ID
HGNC:6614
Subcellular Location
Cytoplasm
Cytoskeleton
Spindle
Cytoplasm
Cytoskeleton
Microtubule organizing center
Centrosome
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000025 , HEK-293T
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0495028
 Compound Info 
N-[2-[benzyl-[4-(phenylsulfamoyl)benzoyl]amino]ethyl]-2-(2-methylpropanoylamino)-1,3-thiazole-5-carboxamide
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C30H31N5O5S2
  1
1
IC50 = 13 nM
 Bioactivity Info 
   TI
   LI
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CP0116716
 Compound Info 
N-[5-[2-(2,6-dichlorophenyl)-5-(difluoromethyl)pyrazol-3-yl]-1,3-thiazol-2-yl]-2-methylpropanamide
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C17H14Cl2F2N4OS
  1
1
IC50 = 17 nM
 Bioactivity Info 
   TI
   LI
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CP0543439
 Compound Info 
N-benzyl-N-[[1-[(2-chlorophenyl)methyl]triazol-4-yl]methyl]-4-(phenylsulfamoyl)benzamide
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C30H26ClN5O3S
  1
1
IC50 = 61 nM
 Bioactivity Info 
   TI
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CP0570991
 Compound Info 
N-benzyl-4-(phenylsulfamoyl)-N-prop-2-ynylbenzamide
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C23H20N2O3S
  1
1
IC50 = 65 nM
 Bioactivity Info 
   TI
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CP0495025
 Compound Info 
N-benzyl-N-but-3-ynyl-4-(phenylsulfamoyl)benzamide
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C24H22N2O3S
  1
1
IC50 = 67 nM
 Bioactivity Info 
   TI
   LI
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CP0495026
 Compound Info 
N-benzyl-N-[(1-benzyltriazol-4-yl)methyl]-4-(phenylsulfamoyl)benzamide
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C30H27N5O3S
  1
1
IC50 = 87 nM
 Bioactivity Info 
   TI
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CP0527895
 Compound Info 
N-benzyl-N-[[1-[(3,4-dichlorophenyl)methyl]triazol-4-yl]methyl]-4-(phenylsulfamoyl)benzamide
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C30H25Cl2N5O3S
  1
1
IC50 = 90 nM
 Bioactivity Info 
   TI
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CP0259778
 Compound Info 
N-benzyl-N-butyl-4-(phenylsulfamoyl)benzamide
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C24H26N2O3S
  1
1
IC50 = 91 nM
 Bioactivity Info 
   TI
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CP0561401
 Compound Info 
N-benzyl-N-(2-phenylethyl)-4-(phenylsulfamoyl)benzamide
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C28H26N2O3S
  1
1
IC50 = 92 nM
 Bioactivity Info 
   TI
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CP0527893
 Compound Info 
N-benzyl-4-(phenylsulfamoyl)-N-[[1-(pyridin-4-ylmethyl)triazol-4-yl]methyl]benzamide
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C29H26N6O3S
  1
1
IC50 = 118 nM
 Bioactivity Info 
   TI
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CP0527892
 Compound Info 
N-benzyl-N-[2-(1-benzyltriazol-4-yl)ethyl]-4-(phenylsulfamoyl)benzamide
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C31H29N5O3S
  1
1
IC50 = 121 nM
 Bioactivity Info 
   TI
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CP0547624
 Compound Info 
N-benzyl-4-[(3-chlorophenyl)sulfamoyl]-N-prop-2-ynylbenzamide
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C23H19ClN2O3S
  1
1
IC50 = 161 nM
 Bioactivity Info 
   TI
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CP0495027
 Compound Info 
N-benzyl-N-[[1-[(2-methyl-1,3-thiazol-4-yl)methyl]triazol-4-yl]methyl]-4-(phenylsulfamoyl)benzamide
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C28H26N6O3S2
  1
1
IC50 = 323 nM
 Bioactivity Info 
   TI
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CP0547625
 Compound Info 
N-benzyl-4-[(3-chlorophenyl)sulfamoyl]-N-(2-cyanoethyl)benzamide
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C23H20ClN3O3S
  1
1
IC50 = 440 nM
 Bioactivity Info 
   TI
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CP0564487
 Compound Info 
N-benzyl-N-[[1-(imidazo[1,2-a]pyrimidin-2-ylmethyl)triazol-4-yl]methyl]-4-(phenylsulfamoyl)benzamide
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C30H26N8O3S
  1
1
IC50 = 573 nM
 Bioactivity Info 
   TI
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CP0543438
 Compound Info 
N-benzyl-N-[[1-[(2-oxo-1,3-oxazolidin-5-yl)methyl]triazol-4-yl]methyl]-4-(phenylsulfamoyl)benzamide
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C27H26N6O5S
  1
1
IC50 = 23900 nM
 Bioactivity Info 
   TI
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CP0543436
 Compound Info 
N-benzyl-4-(benzylsulfamoyl)benzamide
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C21H20N2O3S
  1
1
IC50 > 25000 nM
 Bioactivity Info 
   TI
   LI
   LO
   TS
Cell Line ID: CL000006 , HEK293
 Cell Line Info 
Cell Line ID: CL000017 , HeLa
 Cell Line Info 
Cell Line ID: CL000013 , Sf9
 Cell Line Info 
Biochemical Assays
Compound ID Compound Name Compound Formula
CP0266200
 Compound Info 
(S)-3-(2-methyl-4-(5-methyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)piperazine-1-carboxamido)phenyl dimethylcarbamate
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C22H27N7O3
  3
1 IC50 = 1.2 nM
2 IC50 = 1.82 nM
3 IC50 = 20.89 nM
CP0065872
 Compound Info 
[3-[[4-(aminomethyl)-1-(5-methyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)piperidine-4-carbonyl]amino]phenyl] N,N-dimethylcarbamate
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C23H29N7O3
  6
1 IC50 = 1.6 nM
2 IC50 = 4.3 nM
3 IC50 = 7.5 nM
4 IC50 = 10.72 nM
5 IC50 = 309.03 nM
6 Kd < 1000 nM
CP0495028
 Compound Info 
N-[2-[benzyl-[4-(phenylsulfamoyl)benzoyl]amino]ethyl]-2-(2-methylpropanoylamino)-1,3-thiazole-5-carboxamide
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C30H31N5O5S2
  2
1 IC50 = 5.495 nM
2 IC50 = 7.079 nM
CP0443258
 Compound Info 
(S)-N-(3-(isopropylcarbamoyl)phenyl)-2-methyl-4-(5-methyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)piperazine-1-carboxamide
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C23H29N7O2
  1
1 IC50 = 5.5 nM
CP0576920
 Compound Info 
2-benzyl-6-[(3S)-8-chloro-5-methyl-4-oxo-2,3-dihydro-1,5-benzoxazepin-3-yl]-7-oxo-4,5-dihydropyrazolo[3,4-c]pyridine-3-carboxamide
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C24H22ClN5O4
  2
1 IC50 = 6.31 nM
2 IC50 = 7.943 nM
CP0272822
 Compound Info 
N-benzyl-N-ethyl-4-(phenylsulfamoyl)benzamide
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C22H22N2O3S
  2
1 IC50 = 6.457 nM
2 IC50 = 12.59 nM
CP0550920
 Compound Info 
N-benzyl-N-(2-cyanoethyl)-4-(phenylsulfamoyl)benzamide
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C23H21N3O3S
  2
1 IC50 = 7.244 nM
2 IC50 = 8.913 nM
CP0078319
 Compound Info 
6-(2,4-dichlorophenyl)-8-ethyl-2-(3-fluoro-4-piperazin-1-ylanilino)pyrido[2,3-d]pyrimidin-7-one
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C25H23Cl2FN6O
  2
1 IC50 = 13.49 nM
2 IC50 = 512.86 nM
CP0259778
 Compound Info 
N-benzyl-N-butyl-4-(phenylsulfamoyl)benzamide
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C24H26N2O3S
  3
1 IC50 = 14.45 nM
2 IC50 = 15.49 nM
3 IC50 = 15.5 nM
CP0052763
 Compound Info 
1-(2-hydroxyethyl)-3-[4-(5-methyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)phenyl]-1-phenylurea
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C22H21N5O2
  3
1 IC50 = 24 nM
2 IC50 = 1905.46 nM
3 IC50 > 10000 nM
CP0272823
 Compound Info 
N-[5-[2-(2,6-dichlorophenyl)-5-(difluoromethyl)pyrazol-3-yl]-1,3-thiazol-2-yl]cyclopropanecarboxamide
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C17H12Cl2F2N4OS
  2
1 IC50 = 30.2 nM
2 IC50 = 275.42 nM
CP0116716
 Compound Info 
N-[5-[2-(2,6-dichlorophenyl)-5-(difluoromethyl)pyrazol-3-yl]-1,3-thiazol-2-yl]-2-methylpropanamide
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C17H14Cl2F2N4OS
  3
1 IC50 = 33.11 nM
2 IC50 = 85 nM
3 IC50 = 338.84 nM
CP0061772
 Compound Info 
1-ethyl-3-[5-[6-(4-methoxy-2,6-dimethylphenyl)-2-pyrazin-2-ylpyrimidin-4-yl]-1,3-thiazol-2-yl]urea
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C23H23N7O2S
  4
1 IC50 = 38 nM
2 IC50 = 134.9 nM
3 IC50 = 696 nM
4 IC50 = 707.95 nM
CP0343733
 Compound Info 
N-benzyl-N-methyl-4-[(3-methylphenyl)sulfamoyl]benzamide
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C22H22N2O3S
  2
1 IC50 = 199.53 nM
2 IC50 = 269.15 nM
CP0005166
 Compound Info 
BMS-354825
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C22H26ClN7O2S
  3
1 IC50 = 223.87 nM
2 IC50 = 1513.56 nM
3 Kd = 86 nM
CP0035351
 Compound Info 
(S)-5-fluoro-2-(1-(4-fluorophenyl)ethylamino)-6-(5-methyl-1H-pyrazol-3-ylamino)nicotinonitrile
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C18H16F2N6
  2
1 IC50 = 1949.84 nM
2 IC50 = 6606.93 nM
CP0840908
 Compound Info 
PF-477736
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C22H25N7O2
  2
1 IC50 = 3388.44 nM
2 IC50 > 10000 nM
CP0106652
 Compound Info 
GANDOTINIB
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C23H25ClFN7O
  2
1 IC50 = 6025.6 nM
2 IC50 > 10000 nM
CP0051782
 Compound Info 
1,4,5,6-Tetrahydropyrrolo[3,4-c]pyrazole 18
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C28H35N7O2
  2
1 IC50 = 7943.28 nM
2 IC50 > 10000 nM
CP0127639
 Compound Info 
Chir98014, 20
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C20H17Cl2N9O2
  1
1 IC50 > 10000 nM
CP0575646
 Compound Info 
3-methyl-N-[3-[[3-(trifluoromethyl)phenyl]carbamoyl]phenyl]-1,2-oxazole-5-carboxamide
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C19H14F3N3O3
  1
1 IC50 > 10000 nM
CP0543436
 Compound Info 
N-benzyl-4-(benzylsulfamoyl)benzamide
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C21H20N2O3S
  1
1 IC50 > 50000 nM
Clinical Information about the Protein
Target 1 ( LIM domain kinase-2 (LIMK-2) )
Target Type Literature-reported Target