General Information of the Protein
Protein ID
PT01174
Protein Name
Polymerase acidic protein
Secondarily
Protein Name
RNA-directed RNA polymerase subunit P2
Gene Name
PA
Sequence
MEDFVRQCFNPMIVELAEKTMKEYGEDLKIETNKFAAICTHLEVCFMYSDFHFINEQGESIIVELGDPNALLKHRFEIIEGRDRTMAWTVVNSICNTTGAEKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSEKTHIHIFSFTGEEMATKADYTLDEESRARIKTRLFTIRQEMASRGLWDSFRQSERGEETIEERFEITGTMRKLADQSLPPNFSSLENFRAYVDGFEPNGYIEGKLSQMSKEVNARIEPFLKTTPRPLRLPNGPPCSQRSKFLLMDALKLSIEDPSHEGEGIPLYDAIKCMRTFFGWKEPNVVKPHEKGINPNYLLSWKQVLAELQDIENEEKIPKTKNMKKTSQLKWALGENMAPEKVDFDDCKDVGDLKQYDSDEPELRSLASWIQNEFNKACELTDSSWIELDEIGEDVAPIEHIASMRRNYFTSEVSHCRATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNLYGFIIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHKWEKYCVLEIGDMLIRSAIGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCLLQSLQQIESMIEAESSVKEKDMTKEFFENKSETWPIGESPKGVEESSIGKVCRTLLAKSVFNSLYASPQLEGFSAESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWVLLNASWFNSFLTHALS
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Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Protein Classification
Enzyme
>
Hydrolase
Function
Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.
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Uniprot ID
Primary ID:
P03433

Secondarily ID:
A4GXH3
Q20N31
Q8JUU6
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Subcellular Location
Host cytoplasm
Host nucleus
Map of Molecular Bioactivity Related to the Protein
Map of Molecular Bioactivity Related to the Protein

Protein
Cell Line
Compound

Bioactivity Value:

<= 0.1 μM
> 0.1 μM and <= 10 μM
> 10 μM
Imprecise Activity
Table of Molecular Bioactivities Related to the Protein
Cell Line ID: CL000031 , MDCK
 Cell Line Info 
Compound ID Compound Name Compound Formula
CP0687592
 Compound Info 
(Z)-4-[4-[(4-chlorophenyl)methyl]-1-(cyclohexylmethyl)piperidin-4-yl]-2-hydroxy-4-oxobut-2-enoic acid
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C23H30ClNO4
  1
1
EC50 = 800 nM
 Bioactivity Info 
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CP0109317
 Compound Info 
(Z)-4-[1-benzyl-4-[(4-chlorophenyl)methyl]piperidin-4-yl]-2-hydroxy-4-oxobut-2-enoic acid
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C23H24ClNO4
  1
1
EC50 = 860 nM
 Bioactivity Info 
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CP0115435
 Compound Info 
4-hydroxy-2-oxo-4-(4-phenoxyphenyl)but-3-enoic acid
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C16H12O5
  1
1
EC50 = 1450 nM
 Bioactivity Info 
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CP0410191
 Compound Info 
5-hydroxy-6-oxo-N-[(1R)-1-phenylethyl]-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H15N5O3
  1
1
EC50 = 1500 nM
 Bioactivity Info 
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CP0847636
 Compound Info 
4-hydroxy-2-oxo-4-(4-piperazin-1-ylphenyl)but-3-enoic acid
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C14H16N2O4
  1
1
EC50 = 1500 nM
 Bioactivity Info 
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CP0036531
 Compound Info 
4-hydroxy-2-oxo-4-phenylbut-3-enoic acid
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C10H8O4
  1
1
EC50 = 2070 nM
 Bioactivity Info 
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CP0089024
 Compound Info 
2-hydroxy-4H-isoquinoline-1,3-dione
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C9H7NO3
  1
1
EC50 = 3220 nM
 Bioactivity Info 
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CP0271229
 Compound Info 
5-hydroxy-6-oxo-N-(1-phenylethyl)-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H15N5O3
  1
1
EC50 = 3500 nM
 Bioactivity Info 
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CP0271261
 Compound Info 
5-hydroxy-N-[(1S)-2-hydroxy-1-phenylethyl]-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H15N5O4
  1
1
EC50 = 3500 nM
 Bioactivity Info 
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CP0713308
 Compound Info 
2-Hydroxy-4-(biphenyl-4-yl)-4-oxobut-2-enoic acid
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C16H12O4
  1
1
EC50 = 3560 nM
 Bioactivity Info 
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CP0338376
 Compound Info 
N-(cyclopropylmethyl)-5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C13H13N5O3
  1
1
EC50 = 3660 nM
 Bioactivity Info 
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CP0338858
 Compound Info 
N-[(4-fluorophenyl)methyl]-5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C16H12FN5O3
  1
1
EC50 = 3700 nM
 Bioactivity Info 
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CP0222790
 Compound Info 
5,6-dihydroxy-2-pyrazin-2-ylpyrimidine-4-carboxylic acid
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C9H6N4O4
  1
1
EC50 = 3800 nM
 Bioactivity Info 
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CP0529344
 Compound Info 
N-[(1R)-1-cyclohexylethyl]-5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H21N5O3
  1
1
EC50 = 4851 nM
 Bioactivity Info 
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CP0153974
 Compound Info 
N-benzyl-5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C16H13N5O3
  1
1
EC50 = 5410 nM
 Bioactivity Info 
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CP0160931
 Compound Info 
5-hydroxy-N-[(1R)-1-(4-methylphenyl)ethyl]-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C18H17N5O3
  1
1
EC50 = 5520 nM
 Bioactivity Info 
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CP0257766
 Compound Info 
5-hydroxy-6-oxo-2-pyridin-2-yl-1H-pyrimidine-4-carboxylic acid
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C10H7N3O4
  1
1
EC50 = 5920 nM
 Bioactivity Info 
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CP0711424
 Compound Info 
4-hydroxy-4-(4-methoxyphenyl)-2-oxobut-3-enoic acid
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C11H10O5
  1
1
EC50 = 6700 nM
 Bioactivity Info 
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CP0711425
 Compound Info 
4-hydroxy-2-oxo-4-(4-phenylmethoxyphenyl)but-3-enoic acid
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C17H14O5
  1
1
EC50 = 6800 nM
 Bioactivity Info 
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CP0711426
 Compound Info 
2-Hydroxy-4-oxo-4-o-tolylbut-2-enoic acid
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C11H10O4
  1
1
EC50 = 6800 nM
 Bioactivity Info 
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CP0338859
 Compound Info 
5-hydroxy-6-oxo-N-[(1S)-1-phenylethyl]-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H15N5O3
  1
1
EC50 = 7670 nM
 Bioactivity Info 
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CP0655462
 Compound Info 
4-hydroxy-2-oxo-4-(2,4,6-trimethylphenyl)but-3-enoic acid
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C13H14O4
  1
1
EC50 = 8170 nM
 Bioactivity Info 
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CP0338856
 Compound Info 
N-cyclohexyl-5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C15H17N5O3
  1
1
EC50 = 11480 nM
 Bioactivity Info 
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CP0092193
 Compound Info 
5-hydroxy-2-(1-methylimidazol-4-yl)-6-oxo-1H-pyrimidine-4-carboxylic acid
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C9H8N4O4
  1
1
EC50 = 18090 nM
 Bioactivity Info 
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CP0338857
 Compound Info 
5-hydroxy-N-[(4-methylphenyl)methyl]-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H15N5O3
  1
1
EC50 = 22600 nM
 Bioactivity Info 
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CP0336538
 Compound Info 
5-hydroxy-6-oxo-2-pyrazin-2-yl-N-(1,2,3,4-tetrahydronaphthalen-1-yl)-1H-pyrimidine-4-carboxamide
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C19H17N5O3
  1
1
EC50 = 24300 nM
 Bioactivity Info 
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CP0339214
 Compound Info 
methyl 5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxylate
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C10H8N4O4
  1
1
EC50 = 25460 nM
 Bioactivity Info 
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CP0436403
 Compound Info 
5-hydroxy-N-[(4-methoxyphenyl)methyl]-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H15N5O4
  1
1
EC50 = 35300 nM
 Bioactivity Info 
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CP0336536
 Compound Info 
2-(3-acetamidophenyl)-5-hydroxy-6-oxo-1H-pyrimidine-4-carboxylic acid
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C13H11N3O5
  1
1
EC50 = 58900 nM
 Bioactivity Info 
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CP0338032
 Compound Info 
5-hydroxy-6-oxo-3,7,13-triazatricyclo[7.4.0.02,7]trideca-1(9),2,4,10,12-pentaene-4-carboxylic acid
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C11H7N3O4
  1
1
EC50 = 67400 nM
 Bioactivity Info 
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CP0336537
 Compound Info 
methyl 5-hydroxy-2-(1-methylimidazol-4-yl)-6-oxo-1H-pyrimidine-4-carboxylate
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C10H10N4O4
  1
1
EC50 = 71300 nM
 Bioactivity Info 
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CP0338854
 Compound Info 
N-butyl-5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C13H15N5O3
  1
1
EC50 = 88120 nM
 Bioactivity Info 
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CP0477033
 Compound Info 
1-(4-chlorophenyl)-2-[4-[1-(4-chlorophenyl)-4,5-dihydroimidazol-2-yl]phenyl]-4,5-dihydroimidazole
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C24H20Cl2N4
  1
1
IC50 = 5000 nM
 Bioactivity Info 
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CP0076946
 Compound Info 
(alpha-methyl-1-adamantyl)methylamine
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C12H21N
  1
1
IC50 = 11000 nM
 Bioactivity Info 
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CP0545298
 Compound Info 
1-(4-fluorophenyl)-2-[4-[1-(4-fluorophenyl)-4,5-dihydroimidazol-2-yl]phenyl]-4,5-dihydroimidazole
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C24H20F2N4
  1
1
IC50 = 12000 nM
 Bioactivity Info 
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CP0438845
 Compound Info 
1-(4-methoxyphenyl)-2-[4-[1-(4-methoxyphenyl)-4,5-dihydroimidazol-2-yl]phenyl]-4,5-dihydroimidazole
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C26H26N4O2
  1
1
IC50 = 16000 nM
 Bioactivity Info 
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CP0466718
 Compound Info 
2-[4-(4,5-dihydro-1H-imidazol-2-yl)phenyl]-1-phenyl-4,5-dihydroimidazole
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C18H18N4
  1
1
IC50 = 30000 nM
 Bioactivity Info 
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CP0423707
 Compound Info 
2-[2-[4-(1-pyridin-2-yl-4,5-dihydroimidazol-2-yl)phenyl]-4,5-dihydroimidazol-1-yl]pyridine
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C22H20N6
  1
1
IC50 = 35000 nM
 Bioactivity Info 
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CP0558031
 Compound Info 
2-[4-(4,5-dihydro-1H-imidazol-2-yl)phenyl]-1-(4-methoxyphenyl)-4,5-dihydroimidazole
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C19H20N4O
  1
1
IC50 = 44000 nM
 Bioactivity Info 
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CP0420850
 Compound Info 
2-[4-(4,5-dihydro-1H-imidazol-2-yl)phenyl]-1-(4-fluorophenyl)-4,5-dihydroimidazole
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C18H17FN4
  1
1
IC50 = 65000 nM
 Bioactivity Info 
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CP0423708
 Compound Info 
3-[2-[4-(1-pyridin-3-yl-4,5-dihydroimidazol-2-yl)phenyl]-4,5-dihydroimidazol-1-yl]pyridine
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C22H20N6
  1
1
IC50 = 68000 nM
 Bioactivity Info 
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CP0420851
 Compound Info 
1-phenyl-2-[4-(1-phenyl-4,5-dihydroimidazol-2-yl)phenyl]-4,5-dihydroimidazole
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C24H22N4
  1
1
IC50 = 77000 nM
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Biochemical Assays
Compound ID Compound Name Compound Formula
CP0687592
 Compound Info 
(Z)-4-[4-[(4-chlorophenyl)methyl]-1-(cyclohexylmethyl)piperidin-4-yl]-2-hydroxy-4-oxobut-2-enoic acid
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C23H30ClNO4
  4
1 IC50 = 320 nM
2 IC50 = 330 nM
3 IC50 = 710 nM
4 Ki = 84 nM
CP0109317
 Compound Info 
(Z)-4-[1-benzyl-4-[(4-chlorophenyl)methyl]piperidin-4-yl]-2-hydroxy-4-oxobut-2-enoic acid
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C23H24ClNO4
  4
1 IC50 = 350 nM
2 IC50 = 430 nM
3 IC50 = 2210 nM
4 Ki = 90 nM
CP0713308
 Compound Info 
2-Hydroxy-4-(biphenyl-4-yl)-4-oxobut-2-enoic acid
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C16H12O4
  2
1 IC50 = 3700 nM
2 Ki = 960 nM
CP0089024
 Compound Info 
2-hydroxy-4H-isoquinoline-1,3-dione
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C9H7NO3
  2
1 IC50 = 15000 nM
2 Ki = 850 nM
CP0036531
 Compound Info 
CHEMBL467389
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C10H8O4
  2
1 IC50 = 21300 nM
2 Ki = 480 nM
CP0115435
 Compound Info 
CHEMBL573638
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C16H12O5
  1
1 Ki = 280 nM
CP0410191
 Compound Info 
5-hydroxy-6-oxo-N-[(1R)-1-phenylethyl]-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H15N5O3
  1
1 Ki = 290 nM
CP0847636
 Compound Info 
CHEMBL2040552
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C14H16N2O4
  1
1 Ki = 300 nM
CP0271229
 Compound Info 
5-hydroxy-6-oxo-N-(1-phenylethyl)-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H15N5O3
  1
1 Ki = 720 nM
CP0338376
 Compound Info 
N-(cyclopropylmethyl)-5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C13H13N5O3
  1
1 Ki = 990 nM
CP0271261
 Compound Info 
5-hydroxy-N-[(1S)-2-hydroxy-1-phenylethyl]-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H15N5O4
  1
1 Ki = 1000 nM
CP0222790
 Compound Info 
5,6-dihydroxy-2-pyrazin-2-ylpyrimidine-4-carboxylic acid
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C9H6N4O4
  1
1 Ki = 1100 nM
CP0338858
 Compound Info 
N-[(4-fluorophenyl)methyl]-5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C16H12FN5O3
  1
1 Ki = 1200 nM
CP0529344
 Compound Info 
N-[(1R)-1-cyclohexylethyl]-5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H21N5O3
  1
1 Ki = 1392 nM
CP0160931
 Compound Info 
5-hydroxy-N-[(1R)-1-(4-methylphenyl)ethyl]-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C18H17N5O3
  1
1 Ki = 1610 nM
CP0153974
 Compound Info 
N-benzyl-5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C16H13N5O3
  1
1 Ki = 1650 nM
CP0257766
 Compound Info 
5-hydroxy-6-oxo-2-pyridin-2-yl-1H-pyrimidine-4-carboxylic acid
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C10H7N3O4
  1
1 Ki = 1840 nM
CP0711424
 Compound Info 
CHEMBL2040553
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C11H10O5
  1
1 Ki = 1970 nM
CP0711425
 Compound Info 
CHEMBL492768
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C17H14O5
  1
1 Ki = 1990 nM
CP0711426
 Compound Info 
2-Hydroxy-4-oxo-4-o-tolylbut-2-enoic acid
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C11H10O4
  1
1 Ki = 2000 nM
CP0338859
 Compound Info 
5-hydroxy-6-oxo-N-[(1S)-1-phenylethyl]-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H15N5O3
  1
1 Ki = 2280 nM
CP0655462
 Compound Info 
CHEMBL2040555
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C13H14O4
  1
1 Ki = 2440 nM
CP0338856
 Compound Info 
N-cyclohexyl-5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C15H17N5O3
  1
1 Ki = 3556 nM
CP0092193
 Compound Info 
5-hydroxy-2-(1-methylimidazol-4-yl)-6-oxo-1H-pyrimidine-4-carboxylic acid
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C9H8N4O4
  1
1 Ki = 5010 nM
CP0338857
 Compound Info 
5-hydroxy-N-[(4-methylphenyl)methyl]-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H15N5O3
  1
1 Ki = 7100 nM
CP0339214
 Compound Info 
methyl 5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxylate
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C10H8N4O4
  1
1 Ki = 8000 nM
CP0436403
 Compound Info 
5-hydroxy-N-[(4-methoxyphenyl)methyl]-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
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C17H15N5O4
  1
1 Ki = 11200 nM
CP0336536
 Compound Info 
2-(3-acetamidophenyl)-5-hydroxy-6-oxo-1H-pyrimidine-4-carboxylic acid
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C13H11N3O5
  1
1 Ki = 18700 nM
CP0336537
 Compound Info 
methyl 5-hydroxy-2-(1-methylimidazol-4-yl)-6-oxo-1H-pyrimidine-4-carboxylate
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C10H10N4O4
  1
1 Ki = 20300 nM
CP0338032
 Compound Info 
5-hydroxy-6-oxo-3,7,13-triazatricyclo[7.4.0.02,7]trideca-1(9),2,4,10,12-pentaene-4-carboxylic acid
   Show/Hide
C11H7N3O4
  1
1 Ki = 21600 nM
CP0336538
 Compound Info 
5-hydroxy-6-oxo-2-pyrazin-2-yl-N-(1,2,3,4-tetrahydronaphthalen-1-yl)-1H-pyrimidine-4-carboxamide
   Show/Hide
C19H17N5O3
  1
1 Ki = 24300 nM
CP0338854
 Compound Info 
N-butyl-5-hydroxy-6-oxo-2-pyrazin-2-yl-1H-pyrimidine-4-carboxamide
   Show/Hide
C13H15N5O3
  1
1 Ki = 28570 nM
Clinical Information about the Protein
Target 1 ( Influenza Polymerase acidic endonuclease (Influ PA) )
Target Type Successful Target